Construct: ORF ccsbBroadEn_13310

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006209.1_s300c1, BRDN0000387765
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP54 (159195)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 159195 USP54 ubiquitin specific peptidas... XM_005269582.2 45.8% 45.8% 1_2565del;4000G>C
2 human 159195 USP54 ubiquitin specific peptidas... NM_001350995.2 45.6% 45.6% 1_2445del;3880_4021delinsC
3 human 159195 USP54 ubiquitin specific peptidas... XM_024447852.1 45.6% 45.6% 1_2445del;3880_4021delinsC
4 human 159195 USP54 ubiquitin specific peptidas... XM_024447851.1 44.7% 44.7% 1_2682del;4117G>C
5 human 159195 USP54 ubiquitin specific peptidas... NM_001320437.2 44.6% 44.4% (many diffs)
6 human 159195 USP54 ubiquitin specific peptidas... XM_017015783.1 44.5% 44.4% 1_2565del;4000_4141delinsC
7 human 159195 USP54 ubiquitin specific peptidas... XM_017015784.1 44.5% 44.4% 1_2565del;4000_4141delinsC
8 human 159195 USP54 ubiquitin specific peptidas... XM_017015782.2 44% 44% 1_2616del;4051_4192delinsC
9 human 159195 USP54 ubiquitin specific peptidas... XM_011539368.2 43.9% 43.9% 1_2631del;4066_4207delinsC
10 human 159195 USP54 ubiquitin specific peptidas... XM_024447849.1 43.6% 43.6% 1_2802del;4237G>C
11 human 159195 USP54 ubiquitin specific peptidas... XM_024447848.1 43.4% 43.4% 1_2682del;4117_4258delinsC
12 human 159195 USP54 ubiquitin specific peptidas... XM_024447850.1 43.3% 43.3% 1_2802del;4222_4223insTGCGTACTTCTCAGC
13 human 159195 USP54 ubiquitin specific peptidas... NM_152586.3 43% 42.9% 1_2736del;4171_4312delinsC
14 human 159195 USP54 ubiquitin specific peptidas... XM_017015774.1 43% 42.9% 1_2736del;4171_4312delinsC
15 human 159195 USP54 ubiquitin specific peptidas... XM_017015775.1 43% 42.9% 1_2736del;4171_4312delinsC
16 human 159195 USP54 ubiquitin specific peptidas... XM_017015777.1 43% 42.9% 1_2736del;4171_4312delinsC
17 human 159195 USP54 ubiquitin specific peptidas... XM_024447847.1 43% 42.9% 1_2736del;4171_4312delinsC
18 human 159195 USP54 ubiquitin specific peptidas... XM_024447846.1 42.5% 42.3% (many diffs)
19 human 159195 USP54 ubiquitin specific peptidas... XM_024447832.1 42.4% 42.4% 1_2802del;4237_4378delinsC
20 human 159195 USP54 ubiquitin specific peptidas... XM_024447833.1 42.4% 42.4% 1_2802del;4237_4378delinsC
21 human 159195 USP54 ubiquitin specific peptidas... XM_024447834.1 42.4% 42.4% 1_2802del;4237_4378delinsC
22 human 159195 USP54 ubiquitin specific peptidas... XM_024447835.1 42.4% 42.4% 1_2802del;4237_4378delinsC
23 human 159195 USP54 ubiquitin specific peptidas... XM_024447836.1 42.4% 42.4% 1_2802del;4237_4378delinsC
24 human 159195 USP54 ubiquitin specific peptidas... XM_024447837.1 42.4% 42.4% 1_2802del;4237_4378delinsC
25 human 159195 USP54 ubiquitin specific peptidas... XM_024447838.1 42.4% 42.4% 1_2802del;4237_4378delinsC
26 human 159195 USP54 ubiquitin specific peptidas... XM_024447839.1 42.4% 42.4% 1_2802del;4237_4378delinsC
27 human 159195 USP54 ubiquitin specific peptidas... XM_024447840.1 42.4% 42.4% 1_2802del;4237_4378delinsC
28 human 159195 USP54 ubiquitin specific peptidas... XM_024447841.1 42.4% 42.4% 1_2802del;4237_4378delinsC
29 human 159195 USP54 ubiquitin specific peptidas... XM_024447842.1 42.4% 42.4% 1_2802del;4237_4378delinsC
30 human 159195 USP54 ubiquitin specific peptidas... XM_024447843.1 42.4% 42.4% 1_2802del;4237_4378delinsC
31 human 159195 USP54 ubiquitin specific peptidas... XM_024447844.1 42.4% 42.4% 1_2802del;4237_4378delinsC
32 human 159195 USP54 ubiquitin specific peptidas... XM_024447845.1 42.4% 42.4% 1_2802del;4237_4378delinsC
33 human 159195 USP54 ubiquitin specific peptidas... NR_135250.1 39% (many diffs)
34 human 159195 USP54 ubiquitin specific peptidas... XR_001747048.1 37.8% 1_2331del;3766_3907delinsC;4648_5741del
35 human 159195 USP54 ubiquitin specific peptidas... XR_001747042.1 36.7% 1_2502del;3937_4078delinsC;4819_5912del
36 human 159195 USP54 ubiquitin specific peptidas... NR_146998.2 36.2% 1_2728del;4163G>C;4904_5997del
37 human 159195 USP54 ubiquitin specific peptidas... NR_146997.2 35.4% 1_2728del;4163_4304delinsC;5045_6138del
38 human 159195 USP54 ubiquitin specific peptidas... NR_135249.2 33.9% 1_3132del;4567G>C;5308_6401del
39 human 159195 USP54 ubiquitin specific peptidas... XR_001747041.2 31.8% 1_3407del;4842_4983delinsC;5724_6817del
40 human 159195 USP54 ubiquitin specific peptidas... XR_001747037.2 28.9% 1_4093del;5528_5669delinsC;6410_7503del
41 human 159195 USP54 ubiquitin specific peptidas... XR_001747046.1 27.8% 1_4385del;5820_5961delinsC;6702_7795del
42 human 159195 USP54 ubiquitin specific peptidas... XR_001747043.1 27.7% 1_4556del;5991G>C;6732_7825del
43 human 159195 USP54 ubiquitin specific peptidas... XR_001747035.1 27% 1_4622del;6057_6198delinsC;6939_8032del
44 human 159195 USP54 ubiquitin specific peptidas... XR_001747038.1 25.6% 1_5055del;6490_6631delinsC;7372_8465del
45 human 159195 USP54 ubiquitin specific peptidas... XR_001747047.1 24.3% (many diffs)
46 human 159195 USP54 ubiquitin specific peptidas... XR_001747039.1 24.2% 1_5686del;7121G>C;7862_8955del
47 human 159195 USP54 ubiquitin specific peptidas... XR_002956962.1 24.1% 1_5581del;7016_7157delinsC;7898_8991del
48 human 159195 USP54 ubiquitin specific peptidas... XR_001747044.1 24.1% 1_5686del;7106_7107insTGCGTACTTCTCAGC;7847_8940del
49 human 159195 USP54 ubiquitin specific peptidas... XR_001747036.1 24% 1_5752del;7187G>C;7928_9021del
50 human 159195 USP54 ubiquitin specific peptidas... XR_002956959.1 23.7% 1_5752del;7187_7328delinsC;8069_9162del
51 human 159195 USP54 ubiquitin specific peptidas... XR_002956960.1 20.6% 1_5752del;7172_7173insTGCGTACTTCTCAGC;7386_7387ins526
52 human 159195 USP54 ubiquitin specific peptidas... XR_002956961.1 18.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2244
ORF length:
2175
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGATACAGAG TTTGGGGCCA GTTCTTTCTT CCATTCACCT GCTTCCTGCC
121ATGAGTCACA CTCATCACTA TCTCCAGAGT CATCTGCCCC ACAGCACAGC TCCCCCAGTA
181GATCTGCCTT GAAGCTTCTG ACTTCGGTTG AAGTAGACAA CATTGAACCC TCTGCATTCC
241ACAGGCAAGG TTTACCTAAA GCACCAGGGT GGACTGAGAA GAATTCTCAT CATAGTTGGG
301AGCCATTGGA TGCCCCAGAG GGTAAGCTGC AAGGCTCTAG GTGTGACAAC AGCAGTTGCA
361GCAAGCTCCC TCCACAAGAA GGAAGAGGCA TTGCTCAAGA ACAGCTGTTC CAAGAAAAGA
421AGGATCCTGC TAACCCCTCC CCGGTGATGC CTGGAATAGC CACCTCTGAG AGGGGTGATG
481AACACAGCCT AGGCTGTAGT CCTTCAAATT CATCAGCTCA GCCCAGCCTT CCCCTGTATA
541GAACCTGCCA CCCCATAATG CCTGTTGCTT CTTCATTTGT GCTTCACTGT CCTGATCCTG
601TGCAGAAAAC TAACCAATGC CTCCAAGGCC AAAGCCTCAA AACTTCATTG ACTTTAAAAG
661TGGACAGAGG CAGTGAGGAG ACCTATAGGC CAGAGTTTCC CAGCACAAAG GGGCTTGTCC
721GTTCTCTGGC TGAGCAGTTC CAGAGGATGC AGGGTGTCTC CATGAGGGAT AGTACAGGTT
781TCAAGGATAG AAGTTTGTCA GGTAGTCTAA GGAAGAACTC TTCCCCTTCT GATTCTAAGC
841CTCCTTTCTC ACAGGGTCAA GAGAAAGGCC ACTGGCCATG GGCAAAGCAA CAATCCTCTC
901TGGAGGGTGG GGATAGACCA CTTTCCTGGG AAGAGTCCAC TGAACATTCT TCTCTTGCCT
961TAAACTCTGG GCTGCCTAAT GGTGAAACTT CTAGCGGAGG ACAGCCCAGG TTGGCAGAGC
1021CAGACATATA CCAAGAGAAG CTGTCCCAAG TGAGAGATGT TAGGTCTAAG GATCTGGGCA
1081GCAGTACTGA CTTGGGGACT TCCTTGCCTT TGGATTCCTG GGTGAATATC ACAAGGTTCT
1141GTGATTCTCA GCTTAAGCAT GGGGCACCTA GGCCAGGAAT GAAGTCCTCC CCTCATGATT
1201CCCATACGTG TGTAACCTAT CCAGAGAGAA ATCACATCCT TTTGCATCCA CATTGGAACC
1261AAGACACAGA GCAGGAGACC TCAGAATTGG AGTCTCTGTA TCAGGCCAGT CTTCAGGCTT
1321CTCAAGCTGG CTGTTCTGGA TGGGGGCAGC AGGATACCGC CTGGCACCCA CTTAGCCAAA
1381CAGGCTCTGC AGATGGCATG GGGAGGAGGT TGCACTCAGC CCATGATCCT GGTCTCTCAA
1441AGACTTCAAC AGCAGAAATG GAGCATGGTC TCCATGAAGC CAGAACAGTG CGTACTTCTC
1501AGCATTCATC AAACGTGAGG AAGCCTTTGG AAACCGGGCA CCGTTGTTCC AGCTCCTCTT
1561CCCTCCCTGT CATCCATGAC CCTTCTGTGT TTCTCCTCGG TCCCCAACTC TACCTTCCCC
1621AACCACAGTT CCTGTCCCCA GATGTCCTGA TGCCCACCAT GGCAGGGGAG CCCAATAGAC
1681TCCCAGGAAC TTCAAGGAGT GTCCAGCAGT TTCTGGCTAT GTGTGACAGG GGTGAAACTT
1741CCCAAGGGGC CAAGTACACA GGAAGGACTT TGAACTACCA GAGCCTCCCC CATCGCTCCA
1801GAACAGACAA CTCCTGGGCA CCCTGGTCAG AGACCAACCA GCATATTGGG ACCAGATTCC
1861TGACTACTCC AGGGTGCAAT CCTCAACTAA CCTACACTGC CACACTACCA GAAAGAAGCA
1921AGGGCCTTCA GGTTCCTCAC ACTCAGTCCT GGAGTGATCT TTTCCATTCA CCCTCCCACC
1981CTCCCATTGT TCATCCTGTG TACCCACCAT CTAGCAGTCT TCATGTACCC CTGAGGTCAG
2041CTTGGAATTC AGATCCTGTT CCAGGGTCCC GAACCCCTGG TCCTCGAAGA GTAGATATGC
2101CCCCAGATGA TGACTGGAGG CAAAGCAGTT ATGCCTCCCA CTCTGGACAC AGGAGAACAG
2161TGGGAGAGGG GTTTCTGTTT GTTCTATCAG ATGCTCCCAG AAGAGAGCAG ATCAGGGCTA
2221GAGTCCTGCA GCACAGTCAA TGGTTGCCAA CTTTCTTGTA CAAAGTtggc attataagaa
2281agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)