Construct: ORF ccsbBroadEn_13639

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007143.1_s300c1, BRDN0000396361
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRIM74 (378108)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 378108 TRIM74 tripartite motif containing 74 XM_011516189.2 99.5% 98.7% 37T>C;382G>A;477A>C
2 human 375593 TRIM73 tripartite motif containing 73 NM_198924.3 99.2% 98.8% (many diffs)
3 human 378108 TRIM74 tripartite motif containing 74 NM_001317815.1 99.2% 98.4% (many diffs)
4 human 378108 TRIM74 tripartite motif containing 74 NM_198853.3 99.2% 98.4% (many diffs)
5 human 378108 TRIM74 tripartite motif containing 74 XM_011516187.3 99.2% 98.4% (many diffs)
6 human 135892 TRIM50 tripartite motif containing 50 XM_011515787.1 78.7% 76.3% (many diffs)
7 human 378108 TRIM74 tripartite motif containing 74 XM_011516190.2 68.5% 68% (many diffs)
8 human 135892 TRIM50 tripartite motif containing 50 NM_001281450.1 49.2% 47.7% (many diffs)
9 human 135892 TRIM50 tripartite motif containing 50 XM_011515789.2 49.2% 47.7% (many diffs)
10 human 135892 TRIM50 tripartite motif containing 50 NM_001281451.1 49.1% 47.6% (many diffs)
11 human 135892 TRIM50 tripartite motif containing 50 NM_178125.3 49.1% 47.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
813
ORF length:
747
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TTGGCAGGTG AGCCTGCTGG AGCTGGAGGA CCGGCTTCAG TGTCCCATCT
121GCCTGGAGGT CTTCAAGGAG TCCCTAATGC TACAGTGCGG CCACTCCTAC TGCAAGGGCT
181GCCTGGTTTC CCTGTCCTAC CACCTGGACA CCAAGGTGCG CTGCCCCATG TGCTGGCAGG
241TGGTGGACGG CAGCAGCTCC TTGCCCAACG TCTCCCTGGC CTGGGTGATC GAAGCCCTGA
301GGCTCCCTGG GGACCCGGAG CCCAAGGTCT GCGTGCACCA CCGGAACCCG CTCAGCCTTT
361TCTGCGAGAA GGACCAGGAG CTCATCTGTG GCCTCTGCGG TCTGCTGGGC TCCCACCAAC
421ACCACCCGGT CACGCCCGTC TCCACCATCT GCAGCCGCAT GAAGGAGGAG CTCGCAGCCC
481TCTTCTCTGA GCTGAAGCAG GAGCAGAAGA AGGTGGATGA GCTCATCGCC AAACTGGTGA
541ACAACCGGAC CCGAATCGTC AATGAGTCGG ATGTCTTCAG CTGGGTGATC CGCCGCGAGT
601TCCAGGAGCT GCGCCACCCG GTGGACGAGG AGAAGGCCCG CTGCCTGGAG GGGATAGGGG
661GTCACACCCG TGGCCTGGTG GCCTCCCTGG ACATGCAGCT GGAGCAGGCC CAGGGAACCC
721GGGAGCGGCT GGCCCAAGCC GAGTGTGTGC TGGAACAGTT CGGAAATGAG GACCACCATG
781AGTTCATCTG GTTCCACTCC ATGGCCTCCA GGTACCCAAC TTTCTTGTAC AAAGTtggca
841ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)