Construct: ORF ccsbBroadEn_13923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007533.1_s300c1, BRDN0000392532
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1CB (5500)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5500 | PPP1CB | protein phosphatase 1 catal... | NM_002709.3 | 99.7% | 99% | 201A>G;970delC |
| 2 | human | 5500 | PPP1CB | protein phosphatase 1 catal... | NM_206876.1 | 99.7% | 99% | 201A>G;970delC |
| 3 | mouse | 19046 | Ppp1cb | protein phosphatase 1, cata... | NM_172707.3 | 94.3% | 99% | (many diffs) |
| 4 | mouse | 19046 | Ppp1cb | protein phosphatase 1, cata... | XM_006503780.1 | 57% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GGACGGGGAG CTGAACGTGG ACAGCCTCAT CACCCGGCTG CTGGAGGTAC 121 GAGGATGTCG TCCAGGAAAG ATTGTGCAGA TGACTGAAGC AGAAGTTCGA GGCTTATGTA 181 TCAAGTCTCG GGAGATCTTT CTCAGCCAGC CTATTCTTTT GGAATTGGAA GCACCGCTGA 241 AAATTTGTGG AGATATTCAT GGACAGTATA CAGATTTACT GAGATTATTT GAATATGGAG 301 GTTTCCCACC AGAAGCCAAC TATCTTTTCT TAGGAGATTA TGTGGACAGA GGAAAGCAGT 361 CTTTGGAAAC CATTTGTTTG CTATTGGCTT ATAAAATCAA ATATCCAGAG AACTTCTTTC 421 TCTTAAGAGG AAACCATGAG TGTGCTAGCA TCAATCGCAT TTATGGATTC TATGATGAAT 481 GCAAACGAAG ATTTAATATT AAATTGTGGA AGACCTTCAC TGATTGTTTT AACTGTCTGC 541 CTATAGCAGC CATTGTGGAT GAGAAGATCT TCTGTTGTCA TGGAGGATTG TCACCAGACC 601 TGCAATCTAT GGAGCAGATT CGGAGAATTA TGAGACCTAC TGATGTCCCT GATACAGGTT 661 TGCTCTGTGA TTTGCTATGG TCTGATCCAG ATAAGGATGT GCAAGGCTGG GGAGAAAATG 721 ATCGTGGTGT TTCCTTTACT TTTGGAGCTG ATGTAGTCAG TAAATTTCTG AATCGTCATG 781 ATTTAGATTT GATTTGTCGA GCTCATCAGG TGGTGGAAGA TGGATATGAA TTTTTTGCTA 841 AACGACAGTT GGTAACCTTA TTTTCAGCCC CAAATTACTG TGGCGAGTTT GATAATGCTG 901 GTGGAATGAT GAGTGTGGAT GAAACTTTGA TGTGTTCATT TCAGATATTG AAACCATCTG 961 AAAAGAAAGC TAAATACCAG TATGGTGGAC TGAATTCTGG ACGTCCTGTC ACTCCACCTC 1021 GAACAGCTAA TCCGCGAAGA AAAGGTGCCC AACTTTCTTG TACAAAGTtg gcattataag 1081 aaagcattgc ttatcaattt gttgcaacga ac