Transcript: Human NM_002709.3

Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PPP1CB (5500)
Length:
4916
CDS:
296..1279

Additional Resources:

NCBI RefSeq record:
NM_002709.3
NBCI Gene record:
PPP1CB (5500)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002709.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000080621 CGACAGTTGGTAACCTTATTT pLKO.1 1073 CDS 100% 15.000 21.000 N Ppp1cb n/a
2 TRCN0000323468 CGACAGTTGGTAACCTTATTT pLKO_005 1073 CDS 100% 15.000 21.000 N Ppp1cb n/a
3 TRCN0000338328 CGACAGTTGGTAACCTTATTT pLKO_005 1073 CDS 100% 15.000 21.000 N PPP1CB n/a
4 TRCN0000338388 TTTCTGAATCGTCATGATTTA pLKO_005 995 CDS 100% 13.200 18.480 N PPP1CB n/a
5 TRCN0000338320 TAACTTAAGGAGACGGGTAAA pLKO_005 1394 3UTR 100% 10.800 15.120 N PPP1CB n/a
6 TRCN0000379693 TCAAGTCTCGGGAGATCTTTC pLKO_005 411 CDS 100% 10.800 15.120 N PPP1CB n/a
7 TRCN0000002457 CGGAGAATTATGAGACCTACT pLKO.1 851 CDS 100% 4.050 5.670 N PPP1CB n/a
8 TRCN0000080620 CCCAAATTACTGTGGCGAGTT pLKO.1 1099 CDS 100% 4.050 3.240 N Ppp1cb n/a
9 TRCN0000382202 TGTTTGCTATTGGCTTATAAA pLKO_005 605 CDS 100% 15.000 10.500 N PPP1CB n/a
10 TRCN0000338386 GATGAATGCAAACGAAGATTT pLKO_005 704 CDS 100% 13.200 9.240 N PPP1CB n/a
11 TRCN0000379793 GCTATGGTCTGATCCAGATAA pLKO_005 904 CDS 100% 13.200 9.240 N PPP1CB n/a
12 TRCN0000002458 ACAGCTAATCCGCCGAAGAAA pLKO.1 1253 CDS 100% 5.625 3.938 N PPP1CB n/a
13 TRCN0000002460 CCACAGTACATGAGAATCCTT pLKO.1 2248 3UTR 100% 3.000 2.100 N PPP1CB n/a
14 TRCN0000002461 AGTTTGATAATGCTGGTGGAA pLKO.1 1116 CDS 100% 2.640 1.848 N PPP1CB n/a
15 TRCN0000002459 GAGGAAACCATGAGTGTGCTA pLKO.1 657 CDS 100% 2.640 1.848 N PPP1CB n/a
16 TRCN0000338319 GAGGAAACCATGAGTGTGCTA pLKO_005 657 CDS 100% 2.640 1.848 N PPP1CB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002709.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13923 pDONR223 100% 99.7% 99% None 201A>G;970delC n/a
2 ccsbBroad304_13923 pLX_304 0% 99.7% 99% V5 (not translated due to frame shift) 201A>G;970delC n/a
3 TRCN0000469385 GCCCTAACTTTAGAAGTTCCTTTC pLX_317 31.3% 99.7% 99% V5 (not translated due to frame shift) 201A>G;970delC n/a
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