Transcript: Mouse XM_006503780.1

PREDICTED: Mus musculus protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ppp1cb (19046)
Length:
1179
CDS:
509..1117

Additional Resources:

NCBI RefSeq record:
XM_006503780.1
NBCI Gene record:
Ppp1cb (19046)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006503780.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305503 GTGTATCAAGTCTCGTGAAAT pLKO_005 619 CDS 100% 13.200 18.480 N Ppp1cb n/a
2 TRCN0000080619 GCTGCTATTGTTGATGAGAAA pLKO.1 989 CDS 100% 4.950 3.465 N Ppp1cb n/a
3 TRCN0000305447 GATGAGTGTAAACGAAGATTT pLKO_005 917 CDS 100% 13.200 7.920 N Ppp1cb n/a
4 TRCN0000002459 GAGGAAACCATGAGTGTGCTA pLKO.1 870 CDS 100% 2.640 1.584 N PPP1CB n/a
5 TRCN0000338319 GAGGAAACCATGAGTGTGCTA pLKO_005 870 CDS 100% 2.640 1.584 N PPP1CB n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006503780.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13923 pDONR223 100% 57% 60.2% None (many diffs) n/a
2 ccsbBroad304_13923 pLX_304 0% 57% 60.2% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000469385 GCCCTAACTTTAGAAGTTCCTTTC pLX_317 31.3% 57% 60.2% V5 (not translated due to frame shift) (many diffs) n/a
Download CSV