Construct: ORF ccsbBroadEn_13927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012591.1_s300c1, BRDN0000392141
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP3R1 (5534)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5534 | PPP3R1 | protein phosphatase 3 regul... | NM_000945.4 | 99.6% | 2.3% | 6_7delAAinsC |
| 2 | mouse | 19058 | Ppp3r1 | protein phosphatase 3, regu... | NM_024459.2 | 94.1% | 2.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 132
- ORF length:
- 66
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG CATGAGGCAA GTTATCCTTT GGAAATGTGC TCACACTTTG ATGCGGATGA 121 AATTAAAAGG CTAGGAAAGA GATTTAAGAA GCTTGATTTG GACAATTCTG GTTCTTTGAG 181 TGTGGAAGAG TTCATGTCTC TGCCTGAGTT ACAACAGAAT CCTTTAGTAC AGCGAGTAAT 241 AGATATATTC GACACAGATG GGAATGGAGA AGTAGACTTT AAAGAATTCA TTGAGGGCGT 301 CTCTCAGTTC AGTGTCAAAG GAGATAAGGA GCAGAAATTG AGGTTTGCTT TCCGTATCTA 361 TGACATGGAT AAAGATGGCT ATATTTCCAA TGGGGAACTC TTCCAGGTAT TGAAGATGAT 421 GGTGGGGAAC AATCTGAAAG ATACACAGTT ACAGCAAATT GTAGACAAAA CCATAATAAA 481 TGCAGATAAG GATGGAGATG GAAGAATATC CTTTGAAGAA TTCTGTGCTG TTGTAGGTGG 541 CCTAGATATC CACAAAAAGA TGGTGGTAGA TGTGTGCCCA ACTTTCTTGT ACAAAGTtgg 601 cattataaga aagcattgct tatcaatttg ttgcaacgaa c