Transcript: Human NM_000945.4

Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
PPP3R1 (5534)
Length:
3024
CDS:
406..918

Additional Resources:

NCBI RefSeq record:
NM_000945.4
NBCI Gene record:
PPP3R1 (5534)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000945.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000338284 CCTTTAGTACAGCGAGTAATA pLKO_005 562 CDS 100% 13.200 18.480 N PPP3R1 n/a
2 TRCN0000012485 CGTATCTATGACATGGATAAA pLKO.1 694 CDS 100% 13.200 10.560 N Ppp3r1 n/a
3 TRCN0000280151 CGTATCTATGACATGGATAAA pLKO_005 694 CDS 100% 13.200 10.560 N Ppp3r1 n/a
4 TRCN0000002759 CGTCATGTTGTGGGATCTTTA pLKO.1 2957 3UTR 100% 13.200 10.560 N PPP3R1 n/a
5 TRCN0000350970 CGTCATGTTGTGGGATCTTTA pLKO_005 2957 3UTR 100% 13.200 10.560 N PPP3R1 n/a
6 TRCN0000380415 GAGATTTAAGAAGCTTGATTT pLKO_005 480 CDS 100% 13.200 9.240 N PPP3R1 n/a
7 TRCN0000002758 GATACACAGTTACAGCAAATT pLKO.1 781 CDS 100% 13.200 9.240 N PPP3R1 n/a
8 TRCN0000338336 GATACACAGTTACAGCAAATT pLKO_005 781 CDS 100% 13.200 9.240 N PPP3R1 n/a
9 TRCN0000338337 TGGTAGATGTGTGACTCTTAT pLKO_005 905 CDS 100% 13.200 9.240 N PPP3R1 n/a
10 TRCN0000002756 GAGGTTTGCTTTCCGTATCTA pLKO.1 681 CDS 100% 5.625 3.938 N PPP3R1 n/a
11 TRCN0000338335 GAGGTTTGCTTTCCGTATCTA pLKO_005 681 CDS 100% 5.625 3.938 N PPP3R1 n/a
12 TRCN0000002757 CAGATAAGGATGGAGATGGAA pLKO.1 824 CDS 100% 3.000 2.100 N PPP3R1 n/a
13 TRCN0000002755 CAAAGGAGATAAGGAGCAGAA pLKO.1 657 CDS 100% 4.050 2.430 N PPP3R1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000945.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13927 pDONR223 100% 99.6% 2.3% None 6_7delAAinsC n/a
2 ccsbBroad304_13927 pLX_304 0% 99.6% 2.3% V5 (not translated due to prior stop codon) 6_7delAAinsC n/a
3 TRCN0000471046 TGCGGCTCATGCCCGGTGATCATA pLX_317 70.1% 99.6% 2.3% V5 (not translated due to prior stop codon) 6_7delAAinsC n/a
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