Construct: ORF ccsbBroadEn_14085
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001206.1_s300c1, BRDN0000386014
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TARDBP (23435)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23435 | TARDBP | TAR DNA binding protein | NM_007375.3 | 62.7% | 62.5% | 14T>N;781_1242del |
| 2 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000863.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 3 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000864.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 4 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000865.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 5 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000866.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 6 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000867.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 7 | human | 23435 | TARDBP | TAR DNA binding protein | XM_017000868.2 | 62.7% | 62.5% | 14T>N;781_1242del |
| 8 | human | 643387 | LOC643387 | TAR DNA binding protein pse... | NR_026923.1 | 21.2% | (many diffs) | |
| 9 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_001003899.2 | 79.2% | 84% | (many diffs) |
| 10 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_001003898.3 | 78.4% | 83.2% | (many diffs) |
| 11 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_001008546.2 | 77.3% | 82.1% | (many diffs) |
| 12 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_001008545.2 | 76.8% | 81.5% | (many diffs) |
| 13 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_145556.4 | 56.4% | 59.9% | (many diffs) |
| 14 | mouse | 230908 | Tardbp | TAR DNA binding protein | NM_001305425.1 | 37.7% | 39.8% | (many diffs) |
| 15 | mouse | 230908 | Tardbp | TAR DNA binding protein | XR_390743.3 | 27.2% | (many diffs) | |
| 16 | mouse | 230908 | Tardbp | TAR DNA binding protein | XR_390742.3 | 19.7% | (many diffs) | |
| 17 | mouse | 230908 | Tardbp | TAR DNA binding protein | NR_131120.1 | 12.1% | (many diffs) | |
| 18 | mouse | 230908 | Tardbp | TAR DNA binding protein | NR_027864.1 | 12.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 846
- ORF length:
- 780
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC TGAATATANT CGGGTAACCG AAGATGAGAA CGATGAGCCC ATTGAAATAC 121 CATCGGAAGA CGATGGGACG GTGCTGCTCT CCACGGTTAC AGCCCAGTTT CCAGGGGCGT 181 GTGGGCTTCG CTACAGGAAT CCAGTGTCTC AGTGTATGAG AGGTGTCCGG CTGGTAGAAG 241 GAATTCTGCA TGCCCCAGAT GCTGGCTGGG GAAATCTGGT GTATGTTGTC AACTATCCAA 301 AAGATAACAA AAGAAAAATG GATGAGACAG ATGCTTCATC AGCAGTGAAA GTGAAAAGAG 361 CAGTCCAGAA AACATCCGAT TTAATAGTGT TGGGTCTCCC ATGGAAAACA ACCGAACAGG 421 ACCTGAAAGA GTATTTTAGT ACCTTTGGAG AAGTTCTTAT GGTGCAGGTC AAGAAAGATC 481 TTAAGACTGG TCATTCAAAG GGGTTTGGCT TTGTTCGTTT TACGGAATAT GAAACACAAG 541 TGAAAGTAAT GTCACAGCGA CATATGATAG ATGGACGATG GTGTGACTGC AAACTTCCTA 601 ATTCTAAGCA AAGCCAAGAT GAGCCTTTGA GAAGCAGAAA AGTGTTTGTG GGGCGCTGTA 661 CAGAGGACAT GACTGAGGAT GAGCTGCGGG AGTTCTTCTC TCAGTACGGG GATGTGATGG 721 ATGTCTTCAT CCCCAAGCCA TTCAGGGCCT TTGCCTTTGT TACATTTGCA GATGATCAGA 781 TTGCGCAGTC TCTTTGTGGA GAGGACTTGA TCATTAAAGG AATCAGCGTT CATATATCCA 841 ATGCCTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 901 gttgcaacga ac