Construct: ORF ccsbBroadEn_14218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011581.1_s300c1, BRDN0000395374
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR45B (56270)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56270 | WDR45B | WD repeat domain 45B | XM_005256377.5 | 89% | 85.3% | (many diffs) |
| 2 | human | 56270 | WDR45B | WD repeat domain 45B | NM_019613.4 | 82.8% | 82.5% | (many diffs) |
| 3 | human | 56270 | WDR45B | WD repeat domain 45B | XM_017024862.2 | 78.9% | 78.6% | 0_1ins180;553A>G |
| 4 | human | 56270 | WDR45B | WD repeat domain 45B | XM_017024863.1 | 78.9% | 78.6% | 0_1ins180;553A>G |
| 5 | human | 56270 | WDR45B | WD repeat domain 45B | XM_024450836.1 | 78.9% | 78.6% | 0_1ins180;553A>G |
| 6 | human | 56270 | WDR45B | WD repeat domain 45B | XR_001752565.2 | 33% | (many diffs) | |
| 7 | human | 56270 | WDR45B | WD repeat domain 45B | XR_243524.2 | 31.7% | (many diffs) | |
| 8 | mouse | 66840 | Wdr45b | WD repeat domain 45B | NM_025793.3 | 74.8% | 81.6% | (many diffs) |
| 9 | mouse | 66840 | Wdr45b | WD repeat domain 45B | XM_006533964.3 | 43.2% | 43.7% | (many diffs) |
| 10 | mouse | 66840 | Wdr45b | WD repeat domain 45B | XR_001780040.1 | 32% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 924
- ORF length:
- 858
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 GTGGCATGTT ATTTCGCTGC AAGTATTTAG CTTTAGTTGG TGGTGGAAAA AAGCCGAAAT 121 ACCCTCCCAA CAAAGTAATG ATCTGGGATG ACCTGAAGAA GAAGACTGTT ATTGAAATAG 181 AATTTTCTAC AGAAGTCAAG GCAGTCAAAC TGCGGCGAGA TAGAATTGTG GTGGTTTTGG 241 ACTCCATGAT TAAGGTGTTC ACATTCACAC ACAATCCCCA TCAGTTGCAC GTCTTCGAAA 301 CCTGCTATAA CCCCAAAGGC CTCTGTGTCC TTTGTCCCAA TAGTAACAAC TCCCTCCTGG 361 CCTTTCCGGG CACGCACACG GGCCATGTGC AGCTTGTGGA CCTGGCCAGC ACGGAGAAGC 421 CACCCGTGGA CATTCCTGCA CACGAGGGTG TCCTGAGCTG CATTGCACTC AACCTGCAGG 481 GAACAAGAAT TGCAACTGCA TCCGAGAAAG GGACGCTTAT AAGAATATTT GATACTTCAT 541 CAGGGCATTT AATCCAGGAA CTGCGAAGAG GATCTCAAGC AGCCAATATT TACTGCATCA 601 ACTTCAATCA GGATGCGTCC CTCATCTGCG TATCCAGCGA CCACGGCACA GTGCATATTT 661 TTGCAGCTGA AGATCCAAAA AGGAATAAAC AGTCCAGTTT GGCCTCAGCC AGTTTCCTTC 721 CAAAATACTT CAGTTCCAAG TGGAGTTTCT CCAAGTTTCA GGTTCCCTCA GGCTCTCCGT 781 GCATTTGTGC CTTTGGAGCA GAGCCAAACG CCGTCATTGC AATTTGTGCA GACGGCAGCT 841 ACTACAAATT CCTGTTCAAC CCCAAGGGGG AGTGCATCCG AGATGTCTAC GCGCAGTTTC 901 TAGAGATGAC CGATGACAAG CTGTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa 961 agcattgctt atcaatttgt tgcaacgaac