Construct: ORF ccsbBroadEn_14305

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003035.1_s300c1, BRDN0000457315
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POLR1B (84172)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84172 POLR1B RNA polymerase I subunit B NM_001282774.2 99.8% 99.6% (many diffs)
2 human 84172 POLR1B RNA polymerase I subunit B NM_019014.6 83.7% 82.9% (many diffs)
3 human 84172 POLR1B RNA polymerase I subunit B NM_001282772.2 81% 80.2% (many diffs)
4 human 84172 POLR1B RNA polymerase I subunit B NM_001371969.1 80.4% 79.6% (many diffs)
5 human 84172 POLR1B RNA polymerase I subunit B NM_001137604.3 78.8% 77.9% (many diffs)
6 human 84172 POLR1B RNA polymerase I subunit B NM_001282777.2 71.5% 70.7% (many diffs)
7 human 84172 POLR1B RNA polymerase I subunit B NM_001282779.2 71.5% 70.7% (many diffs)
8 human 84172 POLR1B RNA polymerase I subunit B NM_001371970.1 71.5% 70.7% (many diffs)
9 human 84172 POLR1B RNA polymerase I subunit B NM_001282776.2 65.1% 64.4% (many diffs)
10 human 84172 POLR1B RNA polymerase I subunit B NM_001371971.1 65.1% 64.4% (many diffs)
11 mouse 20017 Polr1b polymerase (RNA) I polypept... XM_006499025.3 74.4% 77.7% (many diffs)
12 mouse 20017 Polr1b polymerase (RNA) I polypept... NM_009086.2 71.3% 76.2% (many diffs)
13 mouse 20017 Polr1b polymerase (RNA) I polypept... XM_006499026.2 51.1% 55.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2925
ORF length:
2856
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGATCCTGGC ATCCGGTGGC GGAACCTGCC CAGCGGGCCT AGCCTAAAGC
121ACTTGACTGA CCCCTCTTAT GGAATCCCGC GGGAACAGCA AAAGGCAGCG TTGCAGGAGC
181TGACGCGGGC GCACGTGGAG TCCTTCAACT ACGCTGTGCA CGAGGGTCTC GGCCTCGCGG
241TGCAGGCTAT ACCTCCCTTT GAATTTGCTT TCAAAGATGA GCGTATCTCT TTTACTATTC
301TGGATGCTGT TATCAGTCCA CCTACAGTTC CAAAAGGGAC CATCTGCAAA GAGGCCAATG
361TTTATCCAGC AGAATGCCGG GGCCGAAGGA GTACCTACCG TGGGAAGTTG ACAGCTGATA
421TCAACTGGGC AGTGAATGGA ATCTCAAAAG GAATCATTAA GCAGTTTCTT GGCTATGTTC
481CCATCATGGT GAAATCCAAG CTTTGCAACT TACGTAACCT TCCCCCACAA GCCCTCATTG
541AGCACCATGA GGAGGCAGAG GAAATGGGGG GCTATTTTAT AATCAATGGC ATTGAAAAAG
601TCATCCGAAT GTTGATTATG CCTCGGAGAA ATTTTCCCAT TGCAATGATA AGACCAAAAT
661GGAAAACCAG AGGGCCTGGT TATACTCAGT ATGGAGTTTC AATGCACTGT GTGAGGGAAG
721AACATTCCGC TGTCAATATG AACCTCCACT ACTTGGAAAA TGGCACAGTT ATGTTGAACT
781TTATTTACCG AAAAGAACTG TTCTTTCTTC CTTTGGGATT TGCACTTAAG GCACTTGTCA
841GCTTTTCTGA TTATCAGATC TTTCAGGAGC TCATCAAAGG AAAAGAGGAT GATTCTTTCC
901TTAGGAACTC TGTTTCTCAG ATGTTAAGGA TTGTAATGGA AGAGGGTTGT TTGACACAAA
961AACAGGTCCT TAACTACCTA GGTGAATGCT TCAGAGTAAA ACTCAATGTT CCTGACTGGT
1021ACCCAAATGA GCAAGCTGCG GAGTTCCTGT TTAACCAGTG CATCTGTATC CACTTGAAAT
1081CCAATACTGA AAAGTTTTAT ATGCTTTGTC TCATGACGCG AAAGCTCTTT GCTTTAGCCA
1141AAGGAGGGTG CATGGAGGAC AATCCTGATA GTTTGGTGAA CCAGGAAGTC CTCACACCGG
1201GTCAGCTCTT CCTTATGTTC CTGAAGGGGT CACTCCCATT GATGGAGCTC CCCACCGATC
1261ATACAGTGTT GAGAGAGAAA AGAATTCCTC CCTGGATGGA AGTGGTCCTT ATACCCATGA
1321CAGGAAAACC AAGTCTGTAC CCAGGATTGT TCCTTTTTAC CACTCCTTGT AGACTGGTAC
1381GGCCTGTGCA GAACTTAGCA TTGGGCAAAG AAGAGCTAAT TGGAACTATG GAACAGATCT
1441TCATGAATGT CGCTATCTTT GAGGATGAAG TTTTTGCTGG AGTTACCACA CACCAGGAAC
1501TCTTTCCACA CAGCCTGCTG AGTGTGATTG CCAACTTCAT CCCTTTCTCT GATCACAACC
1561AGAGTCCACG GAACATGTAC CAATGCCAGA TGGGTAAGCA AACTATGGGC TTTCCACTTC
1621TCACTTATCA AGACCGATCG GATAACAAAC TGTATCGTCT TCAGACTCCT CAGAGTCCCT
1681TGGTGAGACC CTCCATGTAT GATTATTATG ACATGGATAA CTATCCAATT GGGACCAATG
1741CCATCGTTGC TGTGATTTCT TACACTGGCT ATGATATGGA AGATGCCATG ATTGTGAATA
1801AGGCCTCTTG GGAACGAGGC TTTGCCCATG GAAGTGTCTA CAAGTCTGAG TTCATAGACC
1861TCTCTGAAAA AATTAAACAA GGAGATAGTA GCCTGGTGTT TGGCATCAAA CCTGGTGACC
1921CACGCGTTCT GCAGAAGTTA GATGACGATG GATTGCCGTT TATAGGAGCA AAACTGCAGT
1981ACGGAGATCC GTATTACAGC TACCTCAACC TCAACACCGG GGAAAGTTTT GTGATGTACT
2041ATAAGAGTAA AGAAAATTGT GTTGTGGATA ACATCAAAGT GTGCAGTAAT GACACTGGGA
2101GTGGAAAATT CAAGTGTGTT TGCATCACTA TGAGAGTGCC TCGGAACCCA ACTATCGGAG
2161ATAAATTTGC CAGTCGCCAT GGGCAGAAGG GCATTTTAAG CAGATTGTGG CCGGCTGAGG
2221ACATGCCTTT TACTGAGAGT GGGATGGTCC CAGACATTCT GTTCAATCCC CATGGTTTTC
2281CATCCCGCAT GACCATTGGG ATGTTAATTG AGAGTATGGC CGGGAAGTCT GCAGCTTTGC
2341ATGGTCTCTG CCATGATGCT ACACCCTTCA TCTTCTCAGA GGAGAACTCG GCCTTAGAAT
2401ACTTTGGTGA GATGTTAAAG GCTGCTGGCT ACAATTTCTA TGGCACCGAG AGGTTATATA
2461GTGGCATCAG TGGGCTAGAA CTGGAAGCAG ACATCTTCAT AGGAGTGGTT TATTATCAGC
2521GCTTACGCCA TATGGTCTCA GACAAATTTC AAGTAAGGAC AACTGGAGCC CGAGACAGAG
2581TCACCAACCA GCCTATTGGG GGAAGAAATG TCCAGGGTGG AATCCGTTTT GGGGAGATGG
2641AACGGGATGC GCTTTTAGCT CATGGTACAT CTTTTCTCCT TCATGACCGC CTCTTCAACT
2701GCTCAGATCG GTCGGTAGCC CATGTGTGTG TGAAGTGTGG CAGTTTACTC TCTCCACTGT
2761TGGAGAAGCC ACCCCCTTCT TGGTCTGCCA TGCGCAACAG AAAATACAAC TGTACTCTGT
2821GTAGTCGCAG TGACACTATC GATACTGTTT CTGTGCCTTA TGTTTTTCGG TATTTTGTAG
2881CTGAACTGGC AGCTATGAAC ATCAAAGTGA AACTGGATGT TGTTTTGCCA ACTTTCTTGT
2941ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)