Construct: ORF ccsbBroadEn_14437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006315.1_s300c1, BRDN0000385869
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTKN2 (219790)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219790 | RTKN2 | rhotekin 2 | NM_001282941.2 | 99.5% | 98.7% | 468T>N;473A>N |
| 2 | human | 219790 | RTKN2 | rhotekin 2 | XM_017015844.2 | 32% | 31.6% | 468T>N;473A>N;489_1518delinsG |
| 3 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539460.2 | 30.7% | 30.4% | 468T>N;473A>N;489_1578delinsG |
| 4 | human | 219790 | RTKN2 | rhotekin 2 | XM_017015843.1 | 30.6% | 30.3% | 468T>N;473A>N;489_1584delinsG |
| 5 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539457.3 | 29.9% | 29.5% | 468T>N;473A>N;489_1623delinsG |
| 6 | human | 219790 | RTKN2 | rhotekin 2 | XR_945618.2 | 29.5% | (many diffs) | |
| 7 | human | 219790 | RTKN2 | rhotekin 2 | NM_145307.4 | 26.6% | 26.2% | 468T>N;473A>N;489_1827delinsG |
| 8 | human | 219790 | RTKN2 | rhotekin 2 | XM_011539456.2 | 25.1% | 24.8% | 468T>N;473A>N;489_1932delinsG |
| 9 | human | 219790 | RTKN2 | rhotekin 2 | XR_001747053.1 | 7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA GGGGCCGAGC CTGAGGGGTC CTGCGCTCCG CCTGGCGGGG CTTCCCACCC 121 AGCAGGACTG CAACATTCAA GAAAAAATAG ACTTAGAAAT TCGAATGCGA GAAGGAATAT 181 GGAAACTCCT TTCTCTGAGC ACTCAGAAAG ATCAAGTTTT ACATGCAGTT AAGAATCTCA 241 TGGTGTGCAA TGCTCGACTA ATGGCCTATA CATCGGAGCT ACAGAAATTA GAAGAACAGA 301 TTGCAAATCA GACTGGAAGA TGTGATGTGA AATTTGAAAG TAAAGAACGA ACAGCATGTA 361 AAGGAAAGAT TGCCATATCA GATATTCGAA TACCACTAAT GTGGAAAGAC TCTGATCACT 421 TCAGCAATAA AGAACGATCA CGACGCTATG CCATTTTTTG TTTATTCAAA ATGGGAGCTA 481 ATGTGTTTGA TACTGATGTG GTGAATGTGG ATAAAACAAT CACAGATATA TGNTTTGNAA 541 ATGTAACCAT ATTGTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 601 tatcaatttg ttgcaacgaa c