Construct: ORF ccsbBroadEn_14583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018864.1_s300c1, BRDN0000458160
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CHKA (1119)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1119 | CHKA | choline kinase alpha | XM_017017148.2 | 77.3% | 6.9% | (many diffs) |
| 2 | human | 1119 | CHKA | choline kinase alpha | NM_001277.3 | 76.9% | 20.9% | (many diffs) |
| 3 | human | 1119 | CHKA | choline kinase alpha | NM_212469.1 | 73% | 17% | (many diffs) |
| 4 | human | 1119 | CHKA | choline kinase alpha | XM_017017147.1 | 68.7% | 18.5% | (many diffs) |
| 5 | human | 1119 | CHKA | choline kinase alpha | XR_428904.3 | 53.2% | (many diffs) | |
| 6 | human | 1119 | CHKA | choline kinase alpha | XR_949772.2 | 34.2% | (many diffs) | |
| 7 | human | 1119 | CHKA | choline kinase alpha | XR_949773.2 | 32.1% | (many diffs) | |
| 8 | human | 1119 | CHKA | choline kinase alpha | XR_428905.3 | 11.7% | (many diffs) | |
| 9 | mouse | 12660 | Chka | choline kinase alpha | NM_001271496.1 | 68% | 20.9% | (many diffs) |
| 10 | mouse | 12660 | Chka | choline kinase alpha | XM_006531648.2 | 67% | 6.6% | (many diffs) |
| 11 | mouse | 12660 | Chka | choline kinase alpha | XM_006531649.2 | 67% | 6.6% | (many diffs) |
| 12 | mouse | 12660 | Chka | choline kinase alpha | XM_006531650.2 | 67% | 6.6% | (many diffs) |
| 13 | mouse | 12660 | Chka | choline kinase alpha | XM_006531651.3 | 67% | 6.6% | (many diffs) |
| 14 | mouse | 12660 | Chka | choline kinase alpha | XM_011248599.2 | 67% | 6.6% | (many diffs) |
| 15 | mouse | 12660 | Chka | choline kinase alpha | XM_011248600.2 | 67% | 6.6% | (many diffs) |
| 16 | mouse | 12660 | Chka | choline kinase alpha | XM_017318048.1 | 67% | 6.6% | (many diffs) |
| 17 | mouse | 12660 | Chka | choline kinase alpha | XM_017318049.1 | 67% | 6.6% | (many diffs) |
| 18 | mouse | 12660 | Chka | choline kinase alpha | XM_017318050.1 | 67% | 6.6% | (many diffs) |
| 19 | mouse | 12660 | Chka | choline kinase alpha | XM_006531646.1 | 66.5% | 20.4% | (many diffs) |
| 20 | mouse | 12660 | Chka | choline kinase alpha | NM_013490.4 | 64.1% | 17.1% | (many diffs) |
| 21 | mouse | 12660 | Chka | choline kinase alpha | XR_388248.2 | 40.4% | (many diffs) | |
| 22 | mouse | 12660 | Chka | choline kinase alpha | NR_073190.1 | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 444
- ORF length:
- 348
- Sequence:
-
1 GGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 61 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGAA AACCAAATTC TGCACCGGGG 121 GCGAGGACGA GTTCCACATC AGTGTCATCA GAGGCGGCCT TAGCAACATG CTGTTCCAGT 181 GCTCCCTACC TGACACCACA GCCACCCTTG GTGATGAGCC TCGGAAAGTG CTCCTGCGGC 241 TGTATGGAGC GATTTTGCAG ATGAGGTCCT GTAATAAAGA GGGATCCGAA CAAGCTCAGA 301 AAGAAAATGA ATTTCAAGGG GCTGAGGCCA TGGTTCTGGA GAGCGTTATG TTTGCCATTC 361 TCGCAGAGAG GTCACTTGGN CCAAAACTNT ATGGGCATCT TTCCCCAAGG GCCGACTGGA 421 GCAGTTCATC CCGAGCCGGC GATTAGATAC TGANNAATTA AGTTTGCCAG ATATTTCTGC 481 AGAAATCGCC GAGAAAATGG CTACATTTCA TGGTATGAAA ATGCCATTCA ATAAGGNACC 541 AAAATGGCTT TTTGGCACAA TGGAAAAGTA TCTAAAGGAA GTGCTGAGAA TTAAATTTAC 601 TGAGGAATCC AGAATTAAAA AGCTCCACAA ATTGCTCAGT TACAATCTGC CCTTGGAACT 661 GGAAAACCTG AGATCATTGC TTGAATCTAC TCCATCTCCA GTTGTATTTT GTCATAATGA 721 CTGTCAAGAA GGTAATATCT TGTTGCTGGA ANGCCGAGAG AATTCTGAAA AACAGAAACT 781 GATGCTCATT GATTTCGAAT ACAGCAGTTA CANTTACAGG GGATTCGACA TTGGAAATCA 841 CTTCTGTGAG TGGATGTATG ATTATAGCTA TGAAAAATAC CCTTTTTTCA GAGCAAACAT 901 CCNGAAGTAT CCCACCAAGA AACAACAGCT CCATTTTATT TCCAGTTACT TGCCTGCATT 961 CCAAAATGAC TTTGAAAACC TCAGTACTGA AGAAAAATCC ATTATAAAAG AAGAAATGTT 1021 GCTTGAAGTT AATAGGTTTG CCCTTGCATC TCATTTCCTC TGGGGACTGT GGTCCATTGT 1081 ACAAGCCAAG ATTTCATCTA TTGAATTTGG GTACATGGAC TACGCCCAAG CAAGGTTTGA 1141 TGCCTATTTC CACCAGAAGA GGAAGCTTGG GGTGTTGCCA ACTTTCTTGT ACAAAGTTGG 1201 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 1261 TAAAATCATT ATTGCCATCC CAGCTGATTA T