Construct: ORF ccsbBroadEn_14657

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015185.1_s300c1, BRDN0000386943
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNAL (2774)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2774 GNAL G protein subunit alpha L NM_182978.4 99.9% 100% 243G>T
2 human 2774 GNAL G protein subunit alpha L NM_001142339.2 78.6% 75.5% (many diffs)
3 human 2774 GNAL G protein subunit alpha L NM_001261443.1 78.6% 75.5% (many diffs)
4 human 2774 GNAL G protein subunit alpha L NM_001369387.1 78.6% 75.5% (many diffs)
5 human 2774 GNAL G protein subunit alpha L XM_006722324.3 52.3% 52.4% 243G>T;720_721ins654
6 human 2774 GNAL G protein subunit alpha L NM_001261444.2 37.9% 37.9% 0_1ins852
7 mouse 14680 Gnal guanine nucleotide binding ... NM_177137.5 85.3% 91% (many diffs)
8 mouse 14680 Gnal guanine nucleotide binding ... NM_010307.3 73.1% 75.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1440
ORF length:
1374
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG TCTGTGCTAC AGTCTGCGGC CGCTGCTTTT CGGGGGCCCA GGGGACGACC
121CCTGCGCGGC CTCGGagccg ccggtggagg acgcgcagcc cgccccggcc ccggccctgG
181CCCCAGTCCG GGCGGCCGCA AGGGACACGG CCCGGACCCT GCTCCCTCGG GGCGGCGAAG
241GGAGCCCGGC ATGCGCTCGG CCCAAAGCAG ACAAGCCGAA GGAGAAGCGG CAGCGCACCG
301AGCAGCTTAG TGCCGAGGAG CGCGAGGCGG CCAAGGAGCG CGAGGCGGTC AAGGAGGCGA
361GGAAAGTGAG CCGGGGCATC GACCGCATGC TGCGCGACCA GAAGCGCGAC CTGCAGCAGA
421CGCACCGGCT CCTGCTGCTC GGGGCTGGTG AGTCTGGGAA AAGCACTATC GTCAAACAGA
481TGAGGATCCT GCACGTCAAT GGGTTTAATC CCGAGGAAAA GAAACAGAAA ATTCTGGACA
541TCCGGAAAAA TGTTAAAGAT GCTATCGTGA CAATTGTTTC AGCAATGAGT ACTATAATAC
601CTCCAGTTCC GCTGGCCAAC CCTGAAAACC AATTTCGATC AGACTACATC AAGAGCATAG
661CCCCTATCAC TGACTTTGAA TATTCCCAGG AATTCTTTGA CCATGTGAAA AAACTTTGGG
721ACGATGAAGG CGTGAAGGCA TGCTTTGAGA GATCCAACGA ATACCAGCTG ATTGACTGTG
781CACAATACTT CCTGGAAAGA ATCGACAGCG TCAGCTTGGT TGACTACACA CCCACAGACC
841AGGACCTCCT CAGATGCAGA GTTCTGACAT CTGGGATTTT TGAGACACGA TTCCAAGTGG
901ACAAAGTAAA CTTCCACATG TTTGATGTTG GTGGCCAGAG GGATGAGAGG AGAAAATGGA
961TCCAGTGCTT TAACGATGTC ACAGCTATCA TTTACGTCGC AGCCTGCAGT AGCTACAACA
1021TGGTGATTCG AGAAGATAAC AACACCAACA GGCTGAGAGA GTCCCTGGAT CTTTTTGAAA
1081GCATCTGGAA CAACAGGTGG TTACGGACCA TTTCTATCAT CTTGTTCTTG AACAAACAAG
1141ATATGCTGGC AGAAAAAGTC TTGGCAGGGA AATCAAAAAT TGAAGACTAT TTCCCAGAAT
1201ATGCAAATTA TACTGTTCCT GAAGACGCAA CACCAGATGC AGGAGAAGAT CCCAAAGTTA
1261CAAGAGCCAA GTTCTTTATC CGGGACCTGT TTTTGAGGAT CAGCACGGCC ACCGGTGACG
1321GCAAACATTA CTGCTACCCG CACTTCACCT GCGCCGTGGA CACAGAGAAC ATCCGCAGGG
1381TGTTCAACGA CTGCCGCGAC ATCATCCAGC GGATGCACCT CAAGCAGTAT GAGCTCTTGT
1441GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1501acgaac

Download FASTA (ORF) (Full)