Transcript: Human NM_001261444.2

Homo sapiens G protein subunit alpha L (GNAL), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
GNAL (2774)
Length:
5238
CDS:
164..688

Additional Resources:

NCBI RefSeq record:
NM_001261444.2
NBCI Gene record:
GNAL (2774)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001261444.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424620 CACCGGTGACGGCAAACATTA pLKO_005 556 CDS 100% 13.200 18.480 N GNAL n/a
2 TRCN0000115015 GACACGATTCCAAGTGGACAA pLKO.1 130 5UTR 100% 4.050 5.670 N Gnal n/a
3 TRCN0000036482 GCTTTAACGATGTCACAGCTA pLKO.1 213 CDS 100% 2.640 3.696 N GNAL n/a
4 TRCN0000429859 GTAGCTACAACATGGTGATTC pLKO_005 255 CDS 100% 10.800 7.560 N GNAL n/a
5 TRCN0000036483 CCTCAAGCAGTATGAGCTCTT pLKO.1 664 CDS 100% 4.050 2.835 N GNAL n/a
6 TRCN0000036480 GCAAATTATACTGTTCCTGAA pLKO.1 449 CDS 100% 4.050 2.835 N GNAL n/a
7 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1978 3UTR 100% 4.950 2.475 Y ERAP2 n/a
8 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 3464 3UTR 100% 4.950 2.475 Y LOC387873 n/a
9 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 2033 3UTR 100% 4.050 2.025 Y INTS7 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1979 3UTR 100% 13.200 6.600 Y LIAS n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3501 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3501 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001261444.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487707 TGCGATTCTGGCCGACCTTATCAC pLX_317 25.3% 45.3% 45.6% V5 (not translated due to prior stop codon) 0_1ins621;522_523insTGAAAGCT n/a
2 ccsbBroadEn_14657 pDONR223 96.8% 37.9% 37.9% None 0_1ins852 n/a
3 ccsbBroad304_14657 pLX_304 42% 37.9% 37.9% V5 0_1ins852 n/a
4 TRCN0000474275 TCACTACCAGATTTCAAAAGGGCA pLX_317 38.4% 37.9% 37.9% V5 0_1ins852 n/a
5 TRCN0000487797 CAACAAACAGTAACTTGAGACCAT pLX_317 21.1% 37.7% 37.9% V5 (not translated due to prior stop codon) 0_1ins852;522_523insTGAAAGCT n/a
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