Construct: ORF ccsbBroadEn_14662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018881.1_s300c1, BRDN0000458177
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GTF2H1 (2965)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2965 | GTF2H1 | general transcription facto... | NM_001142307.1 | 98.5% | 43.5% | (many diffs) |
| 2 | human | 2965 | GTF2H1 | general transcription facto... | NM_005316.4 | 98.5% | 43.5% | (many diffs) |
| 3 | human | 2965 | GTF2H1 | general transcription facto... | XM_006718208.3 | 98.5% | 43.5% | (many diffs) |
| 4 | human | 2965 | GTF2H1 | general transcription facto... | XM_024448457.1 | 98.5% | 43.5% | (many diffs) |
| 5 | human | 2965 | GTF2H1 | general transcription facto... | XM_024448458.1 | 98.5% | 43.5% | (many diffs) |
| 6 | mouse | 14884 | Gtf2h1 | general transcription facto... | NM_008186.4 | 90.5% | 43% | (many diffs) |
| 7 | mouse | 14884 | Gtf2h1 | general transcription facto... | NM_001291075.1 | 89.6% | 42.5% | (many diffs) |
| 8 | mouse | 14884 | Gtf2h1 | general transcription facto... | XM_006540636.3 | 89.6% | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 914
- ORF length:
- 816
- Sequence:
-
1 CATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GCAACCTCAT CTGAAGAAGT 121 TTTGCTGATT GTAAAGAAAG TGCGTCAAAA GAAGCAGGAT GGAGCTCTGT ACCTCATGGC 181 AGAAAGAATT GCTTGGGCAC CTGAAGGCAA AGATAGATTT ACAATCAGCC ATATGTATGC 241 AGATATTAAA TGCCAGAAAA TTAGTCCAGA AGGAAAAGCT AAAATTCAGC TTCAGCTGGT 301 CCTACATGCA GGGGACACAA CTAACTTCCA TTTTTCCAAT GAAAGCACAG CAGTGAAAGA 361 GCGAGATGCA GTAAAAGACC TTCTTCAGCA GCTGCTGCCC AAATTCAAGA GGAAAGCAAA 421 TAAAGAACTG GAAGAGAAGA ACAGAATGCT GCAAGAAGAT CCTGTTTTGT TTCAGCTTTA 481 TAAAGACCTT GTTGTGAGTC AAGTGATCAG TGCTGAGGAA TTCTGGGCCA ATCGTTTAAA 541 TGTGAATGCA ACAGATAGTT CTTCCACATC CAATCATAAG CAGGATGTTG GCATTTCTGC 601 TGCATTTCTG GCTGATGTCC GGCCCCAAAC TGATGGCTGT AACGGTCTAA GATATAATTT 661 AACTTCTGAT ATCATTGAGT CCATATTTAG GACCTATCCA GCAGTAAAAA TGAAATATGC 721 AGAAAATGTT CCCCACAACA TGACAGAGAA AGAATTCTGG ACACGTTTTT TCCAGTCCCA 781 TTATTTTCAC AGGGATCGGC TGATACAGGT CAAAGGATCT CTTTGCAGAA TGTGCATAGA 841 TGAAAAGCTA AAACATGGTT TCATTAGAGT GAAAACCACT ACTAGATTAA CAGCTTGGAA 901 GATAACCATT AGATGAgggc tatggcattT CCTCTGTGCC ATCTGCTTCC AATTCTTAAT 961 CCCATAAAGA GAAATAGTAA TGCTGCCATC ATCAAGAGAT TTAACCATCA CAGTGCCATG 1021 GTCCTGGCAG CTGGACTCAG AAAACAAGAA GCACAAAATG AACAAACTAG TGAGCCCAGC 1081 AACATGGATG GAAATTCCGG AGATGCAGAC TGCTTTCAGC CAGCAGTCAA AAGGGCGAAA 1141 TTACAAGAGT CCATTGAATA TGAAGACTTG GGGAAAAATA ATTCTGTAAA AACGATTGCA 1201 CTAAACCTCA AGAAGTCAGA TAGGTATTAT CATGGTCCAA CTCCAATCCA GTCACTACAG 1261 TATGCAACAA GTCAGGACAT TATTAATTCT TTTCAAAGTA TTAGACAAGA AATGGAAGCT 1321 TATACACCCA AGTTAACTCA GGTTCTCTCA AGTAGTGCTG CCAGTAGTAC CATCACAGCA 1381 CTGTCACCTG GAGGGGCACT TATGCAGGGA GGAACACAGC AAGCCATAAA CCAGATGGTG 1441 CCAAATGATA TTCAATCTGA ATTGAAACAC TTATATGTAG CTGTTGGAGA ACTTCTACGA 1501 CATTTCTGGT CCTGCTTTCC TGTTAATACG CCATTCCTAG AAGAAAAGGT AGTGAAAATG 1561 AAAAGTAATT TGGAACGATT CCAAGTTACG AAGCTCTGTC CATTCCAAGA AAAGATTCGG 1621 AGACAGTATT TAAGCACAAA TTTGGTAAGT CACATAGAAG AGATGCTCCA GACAGCCTAC 1681 AACAAGCTCC ACACATGGCA GTCACGGCGT CTGATGAAGA AAACGTTGCC AACTTTCTTG 1741 TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT 1801 ATCAGTCAAA ATAAAATCAT TATTGCCATC CCAGCCTGAT AT