Transcript: Human NM_005316.4

Homo sapiens general transcription factor IIH subunit 1 (GTF2H1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
GTF2H1 (2965)
Length:
3012
CDS:
190..1836

Additional Resources:

NCBI RefSeq record:
NM_005316.4
NBCI Gene record:
GTF2H1 (2965)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146791 ATATTTAGGACCTATCCAGC pXPR_003 AGG 602 37% 5 1.0299 GTF2H1 GTF2H1 76598
2 BRDN0001147634 TAGTTGTGTCCCCTGCATGT pXPR_003 AGG 209 13% 3 0.9775 GTF2H1 GTF2H1 76601
3 BRDN0001146264 GAGTCAAGTGATCAGTGCTG pXPR_003 AGG 415 25% 4 0.5734 GTF2H1 GTF2H1 76600
4 BRDN0001146530 GGAAGATAAACCATTAGATG pXPR_003 AGG 832 51% 7 0.4478 GTF2H1 GTF2H1 76599
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005316.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000085942 GCCAATCGTTTAAATGTGAAT pLKO.1 619 CDS 100% 4.950 3.960 N Gtf2h1 n/a
2 TRCN0000288317 GCCAATCGTTTAAATGTGAAT pLKO_005 619 CDS 100% 4.950 3.960 N Gtf2h1 n/a
3 TRCN0000295596 ATGAGCTAAGTTGCAAATATA pLKO_005 2056 3UTR 100% 15.000 10.500 N Gtf2h1 n/a
4 TRCN0000315385 ATTCTGGGCCAATCGTTTAAA pLKO_005 612 CDS 100% 15.000 10.500 N GTF2H1 n/a
5 TRCN0000315380 GATATCATTGAGTCCATATTT pLKO_005 760 CDS 100% 15.000 10.500 N GTF2H1 n/a
6 TRCN0000315381 ATGACCTCACAGGAGTGATAA pLKO_005 1923 3UTR 100% 13.200 9.240 N GTF2H1 n/a
7 TRCN0000006065 GCTGCCATCATCAAGAGATTT pLKO.1 1090 CDS 100% 13.200 9.240 N GTF2H1 n/a
8 TRCN0000315377 GCTGCCATCATCAAGAGATTT pLKO_005 1090 CDS 100% 13.200 9.240 N GTF2H1 n/a
9 TRCN0000006066 CCAACTCCAATCCAGTCACTA pLKO.1 1345 CDS 100% 4.950 3.465 N GTF2H1 n/a
10 TRCN0000006067 CCTACAACAAGCTCCACACAT pLKO.1 1784 CDS 100% 4.950 3.465 N GTF2H1 n/a
11 TRCN0000315378 CCTACAACAAGCTCCACACAT pLKO_005 1784 CDS 100% 4.950 3.465 N GTF2H1 n/a
12 TRCN0000006064 CCTGAATAATAAAGAGGGAAT pLKO.1 2531 3UTR 100% 4.050 2.835 N GTF2H1 n/a
13 TRCN0000006068 GTCCATTGAATATGAAGACTT pLKO.1 1257 CDS 100% 4.950 2.970 N GTF2H1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005316.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14662 pDONR223 99.2% 98.5% 43.5% None (many diffs) n/a
2 ccsbBroad304_14662 pLX_304 0% 98.5% 43.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000469991 GAACACTGGTACGACCTCTTACCG pLX_317 22.2% 98.5% 43.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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