Construct: ORF ccsbBroadEn_14695

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018953.1_s300c1, BRDN0000458249
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP3K5 (4217)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4217 MAP3K5 mitogen-activated protein k... NM_005923.4 93.4% 3% (many diffs)
2 human 4217 MAP3K5 mitogen-activated protein k... XM_017010873.1 86.8% 2.8% (many diffs)
3 human 4217 MAP3K5 mitogen-activated protein k... XM_017010872.1 86.8% 2.8% (many diffs)
4 human 4217 MAP3K5 mitogen-activated protein k... XM_017010871.1 85.6% 2.7% (many diffs)
5 human 4217 MAP3K5 mitogen-activated protein k... XM_017010870.1 85.5% 2.7% (many diffs)
6 human 4217 MAP3K5 mitogen-activated protein k... XM_017010875.1 84.5% 2.8% (many diffs)
7 human 4217 MAP3K5 mitogen-activated protein k... XM_017010874.1 83.2% 2.8% (many diffs)
8 human 4217 MAP3K5 mitogen-activated protein k... XM_017010877.1 82.8% 1.1% (many diffs)
9 human 4217 MAP3K5 mitogen-activated protein k... XM_017010876.1 81.4% 1.1% (many diffs)
10 human 4217 MAP3K5 mitogen-activated protein k... XM_011535839.3 74% 3.2% (many diffs)
11 mouse 26408 Map3k5 mitogen-activated protein k... NM_008580.4 81.7% 2.5% (many diffs)
12 mouse 26408 Map3k5 mitogen-activated protein k... XM_006512739.3 61.2% 3.2% (many diffs)
13 mouse 26408 Map3k5 mitogen-activated protein k... XR_001779529.1 44.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
97
ORF end:
295
ORF length:
198
Sequence:
1AACGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT
61TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGA GCACGGAGGC GGACGAGGGC
121ATCACTTTCT CTGTGCCACC CTTCGCCCCC TCGGGCTTCT GCACCATCCC CGAGGGCGGC
181ATCTGCAGGA GGGGAGGAGC GGCGGCGGTG TGTTTTTTTC ACTATTCAAA CAGGCCATCG
241TCGCCTCCCC GGCACCGTTC ACTCTCAACC AGTCGGTCCG TTCATACTCT TCAGTAAATG
301TGCCATAAGA ATTTAAATGA AATACATGCA ATTCAGGAAT CTTCTTGGGT AGGGAATTTA
361GCTTCACGTG GATAATATCT TGGTCTTTTT CGTAACCCAC TAGATGAATG AGTGCTGGTA
421ATTCTTTCTC TGCCCCAACT AATTCTTCAG TACTCTCGAG TCCTCTAGTC ACATCATTCT
481AAACTGCACA TTCAGTGACA TGTGAGCACT CCGGCAATCA TGAGATACAC TACAACTGAG
541CGTGCTCATG CCTactggtg gtggccgaga gcgaggccct gcagagcttg cgggaggcgt
601gcgagacagt gggcgccacc ctggaaaccc tgcattttgg gaaactcgac tttggagaaa
661ccaccgtgct ggaccgcttt tacaatgcag atattgcggt ggtggagatg agcgatgcct
721tccggcagcc gtccttgttt taccaccttg gggtgagaga aagtttcagc atggccaaca
781acatcatcct ctactgtgat actaactcgg actctctgca gtcactgaag gaaatcattt
841gccagaagaa tactatgtgc actgggaact acacctttgt tccttacatg ataactccac
901ataacaaagt ctactgctgt gacagcagct tcatgaaggg gttgacagag ctcatgcaac
961cgaacttcga gctgcttctt ggacccatct gcttacctct tgtggatcgt tttattcaac
1021ttttgaaggt ggcacaagca agttctagcc agtacttccg ggaatctata ctcaatgaca
1081tcaggaaagc tcgtaattta tacactggta aagaattggc agctgagttg gcaagaattc
1141ggcagcgagt agataatatc gaagtcttga cagcagatat tgtcataaat ctgttacttt
1201cctacagaga tatccaggac tatgattcta ttgtgaagct ggtagagact ttagaaaaac
1261tgccaacctt tgatttggcc tcccatcacc atgtgaagtt tcattatgca tttgcactga
1321ataggagaaa tctccctggt gacagagcaa aagctcttga tattatgatt cccatggtgc
1381aaagcgaagg acaagttgct tcagatatgt attgcctagt tggtcgaatc tacaaagata
1441tgtttttgga ctctaatttc acggacactg aaagcagaga ccatggagct tcttggttca
1501aaaaggcatt tgaatctgag ccaacactac agtcaggaat taattatgcg gtcctcctcc
1561tggcagctgg acaccagttt gaatcttcct ttgagctccg gaaagttggg gtgaagctaa
1621gtagtcttct tggtaaaaag ggaaacttgg aaaaactcca gagctactgg gaagttggat
1681tttttctggg ggccagcgtc ctagccaatg accacatgag agtcattcaa gcatctgaaa
1741agctttttaa actgaagaca ccagcatggt acctcaagtc tattgtagag acaattttaa
1801tatataagca ttttgtgaaa ctgaccacag aacagcctgt ggccaagcaa gaacttgtgg
1861acttttggat ggatttcctg gtcgaggcca caaagacaga tgttactgtg gttaggtttc
1921cagtattaat attagaacca accaaaatct atcaaccttc ttatttgtct atcaacaatg
1981aagttgagga aaagacaatc tctatttggc acgtgcttcc tgatgacaag aaaggtatac
2041atgagtggaa ttttagtgcc tcttctgtca ggggagtgag tatttctaaa tttgaagaaa
2101gatgctgctt tctttatgtg cttcacaatt ctgatgattt ccaaatctat ttctgtacag
2161aacttcattg taaaaagttt tttgagatgg tgaacaccat taccgaagag aaggggagaa
2221gcacagagga aggagactgt gaaagtgact tgctggagta tgactatgaa tatgatgaaa
2281atggtgacag agtcgtttta ggaaaaggca cttatgggat agtctacgca ggtcgggact
2341tgagcaacca agtcagaatt gctattaagg aaatcccaga gagagacagc agatactctc
2401agcccctgca tgaagaaata gcattgcata aacacctgaa gcacaaaaat attgtccagt
2461atctgggctc tttcagtgag aatggtttca ttaaaatctt catggagcag gtccctggag
2521gaagtctttc tgctctcctt cgttccaaat ggggtccatt aaaagacaat gagcaaacaa
2581ttggctttta tacaaagcaa atactggaag gattaaaata tctccatgac aatcagatag
2641ttcaccggga cataaagggt gacaatgtgt tgattaatac ctacagtggt gttctcaaga
2701tctctgactt cggaacatca aagaggcttg ctggcataaa cccctgtact gaaactttta
2761ctggtaccct ccagtatatg gcaccagaaa taatagataa aggaccaaga ggctacggaa
2821aagcagcaga catctggtct ctgggctgta caatcattga aatggccaca ggaaaacccc
2881cattttatga actgggagaa ccacaagcag ctatgttcaa ggtgggaatg tttaaagtcc
2941accctgagat cccagagtcc atgtctgcag aggccaaggc attcatactg aaatgttttg
3001aaccagatcc tgacaagaga gcctgtgcta acgacttgct tgttgatgag tttttaaaag
3061tttcaagcaa aaagaaaaag acacaaccta agctttcagc tctttcagct ggatcaaatg
3121aatatctcag gagtatatcc ttgccggtac ctgtgctggt ggaggacacc agcagcagca
3181gtgagtacgg ctcagtttca cccgacacgg agttgaaagt ggaccccttc tctttcaaaa
3241caagagccaa gtcctgcgga gaaagagatg tcaagggaat tcggacactc tttttgggca
3301ttccagatga gaattttgaa gatcacagtg ctcctccttc ccctgaagaa aaagattctg
3361gattcttcat gctgaggaag gacagtgaga ggcgagctac ccttcacagg atcctgacgg
3421aagaccaaga caaaattgtg agaaacctaa tggaatcttt agctcagggg gctgaagaac
3481cgaaactaaa atgggaacac atcacaaccc tcattgcaag cctcagagaa tttgtgagat
3541ccactgaccg aaaaatcata gccaccacac tgtcaaagct gaaactggag ctggacttcg
3601acagccatgg cattagccaa gtccaggtgg tactctttgg ttttcaagat gctgtcaata
3661aagttcttcg gaatcataac atcaagccgc actggatgtt tgccttagac agtatcattc
3721ggaaggcggt acagacagcc attaccatcc tggttccaga actaaggcca catttcagcc
3781ttgcatctga gagtgatact gctgatcaag aagacttgga tgtagaagat gaccatgagg
3841aacagccttc aaatcaaact gtccgaagac ctcaggctgt cattgaagat gctgtggcta
3901cctcaggcgt gagcacgctc agttctactg tgtctcatga ttcccagagt gctcaccggt
3961cactgaatgt acagcttgga aggatgaaaa tagaaaccaa tagattactg gaagaattgg
4021ttcggaaaga gaaagaatta caagcactcc ttcatcgagc tattgaagaa aaagaccaag
4081aaattaaaca cctgaagctt aagtcccaac ccatagaaat tcctgaattg cctgtatttc
4141atctaaattc ttctggcaca aatactgaag attctgaact taccgactgg ctgagagtga
4201atggagctga tgaagacact ataagccggt ttttggctga agattataca ctattggatg
4261ttctctacta tgttacacgt gatgacttaa aatgcttgag actaagggga gggatgctgt
4321gcacactgtg gaaggctatc attgactttc gaaacaaaca gactttgcca actttcttgt
4381acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

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