Construct: ORF ccsbBroadEn_14695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018953.1_s300c1, BRDN0000458249
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K5 (4217)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4217 | MAP3K5 | mitogen-activated protein k... | NM_005923.4 | 93.4% | 3% | (many diffs) |
| 2 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010873.1 | 86.8% | 2.8% | (many diffs) |
| 3 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010872.1 | 86.8% | 2.8% | (many diffs) |
| 4 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010871.1 | 85.6% | 2.7% | (many diffs) |
| 5 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010870.1 | 85.5% | 2.7% | (many diffs) |
| 6 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010875.1 | 84.5% | 2.8% | (many diffs) |
| 7 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010874.1 | 83.2% | 2.8% | (many diffs) |
| 8 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010877.1 | 82.8% | 1.1% | (many diffs) |
| 9 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010876.1 | 81.4% | 1.1% | (many diffs) |
| 10 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_011535839.3 | 74% | 3.2% | (many diffs) |
| 11 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | NM_008580.4 | 81.7% | 2.5% | (many diffs) |
| 12 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XM_006512739.3 | 61.2% | 3.2% | (many diffs) |
| 13 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XR_001779529.1 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 295
- ORF length:
- 198
- Sequence:
-
1 AACGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGA GCACGGAGGC GGACGAGGGC 121 ATCACTTTCT CTGTGCCACC CTTCGCCCCC TCGGGCTTCT GCACCATCCC CGAGGGCGGC 181 ATCTGCAGGA GGGGAGGAGC GGCGGCGGTG TGTTTTTTTC ACTATTCAAA CAGGCCATCG 241 TCGCCTCCCC GGCACCGTTC ACTCTCAACC AGTCGGTCCG TTCATACTCT TCAGTAAATG 301 TGCCATAAGA ATTTAAATGA AATACATGCA ATTCAGGAAT CTTCTTGGGT AGGGAATTTA 361 GCTTCACGTG GATAATATCT TGGTCTTTTT CGTAACCCAC TAGATGAATG AGTGCTGGTA 421 ATTCTTTCTC TGCCCCAACT AATTCTTCAG TACTCTCGAG TCCTCTAGTC ACATCATTCT 481 AAACTGCACA TTCAGTGACA TGTGAGCACT CCGGCAATCA TGAGATACAC TACAACTGAG 541 CGTGCTCATG CCTactggtg gtggccgaga gcgaggccct gcagagcttg cgggaggcgt 601 gcgagacagt gggcgccacc ctggaaaccc tgcattttgg gaaactcgac tttggagaaa 661 ccaccgtgct ggaccgcttt tacaatgcag atattgcggt ggtggagatg agcgatgcct 721 tccggcagcc gtccttgttt taccaccttg gggtgagaga aagtttcagc atggccaaca 781 acatcatcct ctactgtgat actaactcgg actctctgca gtcactgaag gaaatcattt 841 gccagaagaa tactatgtgc actgggaact acacctttgt tccttacatg ataactccac 901 ataacaaagt ctactgctgt gacagcagct tcatgaaggg gttgacagag ctcatgcaac 961 cgaacttcga gctgcttctt ggacccatct gcttacctct tgtggatcgt tttattcaac 1021 ttttgaaggt ggcacaagca agttctagcc agtacttccg ggaatctata ctcaatgaca 1081 tcaggaaagc tcgtaattta tacactggta aagaattggc agctgagttg gcaagaattc 1141 ggcagcgagt agataatatc gaagtcttga cagcagatat tgtcataaat ctgttacttt 1201 cctacagaga tatccaggac tatgattcta ttgtgaagct ggtagagact ttagaaaaac 1261 tgccaacctt tgatttggcc tcccatcacc atgtgaagtt tcattatgca tttgcactga 1321 ataggagaaa tctccctggt gacagagcaa aagctcttga tattatgatt cccatggtgc 1381 aaagcgaagg acaagttgct tcagatatgt attgcctagt tggtcgaatc tacaaagata 1441 tgtttttgga ctctaatttc acggacactg aaagcagaga ccatggagct tcttggttca 1501 aaaaggcatt tgaatctgag ccaacactac agtcaggaat taattatgcg gtcctcctcc 1561 tggcagctgg acaccagttt gaatcttcct ttgagctccg gaaagttggg gtgaagctaa 1621 gtagtcttct tggtaaaaag ggaaacttgg aaaaactcca gagctactgg gaagttggat 1681 tttttctggg ggccagcgtc ctagccaatg accacatgag agtcattcaa gcatctgaaa 1741 agctttttaa actgaagaca ccagcatggt acctcaagtc tattgtagag acaattttaa 1801 tatataagca ttttgtgaaa ctgaccacag aacagcctgt ggccaagcaa gaacttgtgg 1861 acttttggat ggatttcctg gtcgaggcca caaagacaga tgttactgtg gttaggtttc 1921 cagtattaat attagaacca accaaaatct atcaaccttc ttatttgtct atcaacaatg 1981 aagttgagga aaagacaatc tctatttggc acgtgcttcc tgatgacaag aaaggtatac 2041 atgagtggaa ttttagtgcc tcttctgtca ggggagtgag tatttctaaa tttgaagaaa 2101 gatgctgctt tctttatgtg cttcacaatt ctgatgattt ccaaatctat ttctgtacag 2161 aacttcattg taaaaagttt tttgagatgg tgaacaccat taccgaagag aaggggagaa 2221 gcacagagga aggagactgt gaaagtgact tgctggagta tgactatgaa tatgatgaaa 2281 atggtgacag agtcgtttta ggaaaaggca cttatgggat agtctacgca ggtcgggact 2341 tgagcaacca agtcagaatt gctattaagg aaatcccaga gagagacagc agatactctc 2401 agcccctgca tgaagaaata gcattgcata aacacctgaa gcacaaaaat attgtccagt 2461 atctgggctc tttcagtgag aatggtttca ttaaaatctt catggagcag gtccctggag 2521 gaagtctttc tgctctcctt cgttccaaat ggggtccatt aaaagacaat gagcaaacaa 2581 ttggctttta tacaaagcaa atactggaag gattaaaata tctccatgac aatcagatag 2641 ttcaccggga cataaagggt gacaatgtgt tgattaatac ctacagtggt gttctcaaga 2701 tctctgactt cggaacatca aagaggcttg ctggcataaa cccctgtact gaaactttta 2761 ctggtaccct ccagtatatg gcaccagaaa taatagataa aggaccaaga ggctacggaa 2821 aagcagcaga catctggtct ctgggctgta caatcattga aatggccaca ggaaaacccc 2881 cattttatga actgggagaa ccacaagcag ctatgttcaa ggtgggaatg tttaaagtcc 2941 accctgagat cccagagtcc atgtctgcag aggccaaggc attcatactg aaatgttttg 3001 aaccagatcc tgacaagaga gcctgtgcta acgacttgct tgttgatgag tttttaaaag 3061 tttcaagcaa aaagaaaaag acacaaccta agctttcagc tctttcagct ggatcaaatg 3121 aatatctcag gagtatatcc ttgccggtac ctgtgctggt ggaggacacc agcagcagca 3181 gtgagtacgg ctcagtttca cccgacacgg agttgaaagt ggaccccttc tctttcaaaa 3241 caagagccaa gtcctgcgga gaaagagatg tcaagggaat tcggacactc tttttgggca 3301 ttccagatga gaattttgaa gatcacagtg ctcctccttc ccctgaagaa aaagattctg 3361 gattcttcat gctgaggaag gacagtgaga ggcgagctac ccttcacagg atcctgacgg 3421 aagaccaaga caaaattgtg agaaacctaa tggaatcttt agctcagggg gctgaagaac 3481 cgaaactaaa atgggaacac atcacaaccc tcattgcaag cctcagagaa tttgtgagat 3541 ccactgaccg aaaaatcata gccaccacac tgtcaaagct gaaactggag ctggacttcg 3601 acagccatgg cattagccaa gtccaggtgg tactctttgg ttttcaagat gctgtcaata 3661 aagttcttcg gaatcataac atcaagccgc actggatgtt tgccttagac agtatcattc 3721 ggaaggcggt acagacagcc attaccatcc tggttccaga actaaggcca catttcagcc 3781 ttgcatctga gagtgatact gctgatcaag aagacttgga tgtagaagat gaccatgagg 3841 aacagccttc aaatcaaact gtccgaagac ctcaggctgt cattgaagat gctgtggcta 3901 cctcaggcgt gagcacgctc agttctactg tgtctcatga ttcccagagt gctcaccggt 3961 cactgaatgt acagcttgga aggatgaaaa tagaaaccaa tagattactg gaagaattgg 4021 ttcggaaaga gaaagaatta caagcactcc ttcatcgagc tattgaagaa aaagaccaag 4081 aaattaaaca cctgaagctt aagtcccaac ccatagaaat tcctgaattg cctgtatttc 4141 atctaaattc ttctggcaca aatactgaag attctgaact taccgactgg ctgagagtga 4201 atggagctga tgaagacact ataagccggt ttttggctga agattataca ctattggatg 4261 ttctctacta tgttacacgt gatgacttaa aatgcttgag actaagggga gggatgctgt 4321 gcacactgtg gaaggctatc attgactttc gaaacaaaca gactttgcca actttcttgt 4381 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c