Construct: ORF ccsbBroadEn_14706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004355.1_s300c1, BRDN0000457292
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYO1E (4643)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4643 | MYO1E | myosin IE | NM_004998.4 | 99.6% | 20% | (many diffs) |
| 2 | mouse | 71602 | Myo1e | myosin IE | NM_181072.3 | 88.5% | 19.3% | (many diffs) |
| 3 | mouse | 71602 | Myo1e | myosin IE | XM_011242814.1 | 87.5% | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGGAAGCAAA GGTGTCTACC AGTACCACTG GCAAAGCCAC AATGTCAAGC 121 ACAGTGGTGT GGACGACATG GTGCTACTGT CCAAGATCAC AGAGAACTCC ATCGTGGAGA 181 ATCTGAAGAA GAGATACATG GATGACTACA TTTTTACATA TATAGGATCT GTATTAATCT 241 CAGTCAACCC TTTCAAGCAG ATGCCATATT TTGGGGAAAA GGAAATTGAA ATGTACCAAG 301 GAGCGGCACA GTATGAAAAC CCACCACATA TCTATGCCCT TGCAGATAAT ATGTACAGAA 361 ACATGATCAT TGACAGAGAG AACCAGTGCG TCATTATCAG TGGTGAAAGT GGTGCTGGAA 421 AAACAGTGGC TGCCAAATAT ATCATGAGCT ACATCTCCAG AGTGTCTGGA GGAGGGACCA 481 AAGTCCAGCA CGTGAAGGAC ATTATCCTGC AGTCCAACCC GCTGCTGGAG GCCTTCGGGA 541 ACGCCAAGAC CGTCCGGAAC AACAACTCCA GCCGATTTGG AAAATACTTT GAAATCCAGT 601 TCAGTCCAGG TGGGGAACCA GATGGTGGAA AGATCTCCAA CTTCCTTCTG GAAAAATCTA 661 GGGTGGTGAT GAGGAACCCA GGAGAGCGGA GTTTTCACAT ATTTTACCAG CTCATCGAGG 721 GCGCCTCTGC AGAGCCAGCA AACACAGCCT TGGCATCACC AGCATGGACT ATTATTACTA 781 CCTGAGCCTC TCGGGCTCAT ACAAGGTTGA TGACATTGAC GACAGGCGGG AGTTTCAGGA 841 AACTCTGCAC GCCATGAATG TGATTGGGAT CTTTGCAGAA GAGCAAACGC TGGTGTTGCA 901 GATAGTGGCG GGTATTCTCC ACCTGGGAAA CATCAGCTTC AAAGAAGTTG GCAACTACGC 961 GGCTGTGGAG AGTGAAGAGT TTTTAGCTTT TCCTGCATAT CTGCTAGGGA TAAACCAGGA 1021 CCGGTTGAAA GAAAAGCTAA CAAGCCGGCA GATGGATAGC AAGTGGGGAG GCAAATCCGA 1081 ATCCATCCAC GTGACCCTCA ACGTAGAGCA GGCCTGTTAC ACCCGGGATG CGCTCGCCAA 1141 GGCCCTGCAC GCCCGGGTCT TTGATTTCTT GGTAGATTCC ATCAATAAAG CCATGGAGAA 1201 AGACCATGAA GAATACAACA TTGGCGTCCT AGACATCTAT GGCTTTGAAA TATTCCAGAA 1261 AAATGGCTTT GAACAGTTTT GTATCAATTT tgttaatgaa aaactgcagc agatttttat 1321 tgaactgaca ttaaaggcag aacaggaaga atatgttcaa gagggaataa gatggacacc 1381 cattgagtac tttaataata aaatcgtatg tgacctcata gagaacaaag tgaaccctcc 1441 tggcatcatg agcatcctgg aTGACGTGTG CGCCACGATG CATGCGGTGG GTGAGGGGGC 1501 AGGATCAGAC GCTGCTCCAG AAACTTCAGA TGCAGATTGG GAGTCATGAG CACTTCAACA 1561 GTTGGAACCA AGGCTTCATC ATTCATCATT ATGCTGGGAA GGTATCCTAT GACATGGATG 1621 GCTTTTGTGA AAGGAACCGG GATGTGCTTT TTATGGATCT CATCGAGCTT ATGCAGAGCA 1681 GCGAGCTGCC TTTCATAAAG TCTTTATTTC CGGAAAATCT GCAGGCTGAC AAGAAAGGGC 1741 GCCCAACTAC TGCCGGAAGC AAAATCAAAG AAACAAGCCA ATGACCTTGT GAGCACCCTG 1801 ATTGAAATGT ACGCCCCACT ACATTCGCTG CAATCAAGCC AAACGAAACC AAGAAGCCCA 1861 GAGACTGGGA GGAAAGCAGG GTAAAGCATC AAGTCGAATA TTTGGGTCTG AAAGAGAACA 1921 TTCGAGTGAG AAGAGCTGGC TATGCCTATC GGCGCATCTT CCAAAAATTC CTACAGAGGT 1981 ATGCCATTCT GACCAAAGCC ACCTGGCCTT CTTGGCAGGG AGAGGAGAAG CAAGGCGTCC 2041 TGCACCTGCT GCAGTCGGTC AACATGGACA GCGACCAGTT CCAGCTGGGG AGGAGTAAag 2101 tgttcatcaa agcccccgag tctctatttc ttttagaaga gatgagagag agaaagtatg 2161 atgggtatgc tcgagtgata caGAAATCAT GGAGGAAATT CGTGGCCCGG AAGAAATACG 2221 TTCAAATGAG AGAAGAAGCC TCAGACCTCT TATTGAACAA GAAGGAGAGA AGGAGAAACA 2281 GTATTAACAG GAACTTTATA GGGGATTATA TTGGGATGGA AGAGCACCCA GAACTCCAGC 2341 AGTTCGTGGG CAAGAGGGAG AAGATTGATT TCGCAGACAC AGTCACCAAG TATGACAGGA 2401 GGTTCAAGGG TGTAAAGCGA GAACCTGACT CCTTACCCCA AAGTGCTTGT ACTTAATCGG 2461 ACGAGAAAAA GCTCAAACAG GGCCCAGACA AGGGCCTGGT GAAAGAAGTC CTGAAGCGGA 2521 AAATCGAGAT AGAACGGATC TTGTCATGTG CTCCCTCAGT ACTATGCAGG ATGACATTTT 2581 TATTCTCCAT GAGCAAGAGT ATGACAGTTT GCTTGAATCT GTCTTCAAAA CTGAATTCCT 2641 AAGCCTCTTA GCAAAGCGTT ACGAGGAGAA GACCCAGAAG Caactacctc tgaaattcag 2701 caatacgctt gaactgaagt tgaaaaagga aaactggggc ccctggagtg cagggggctc 2761 ccggcaagtg cagttccacc aagggtttgg ggACCTGGCT GTCCTCAAGC CCAGTAACAA 2821 AGTGCTGCAG GTCAGCATCG GACCTGGACT GCCCAAGAAC TCCCGTCCTA CCAGAAGGAA 2881 CACTACCCAA AATACAGGTT ATTCCAGTGG GACTCAAAAT GCCAACTACC CAGTGAGAGC 2941 TGCCCCTCCT CCCCCAGGAT ACCATCAGAA CGGAGTCATC AGAAACCAGT ATGTGCCATA 3001 TCCCCATCGC TCCTGGAAGC CAGAGGTCCA ATCAGAAAAG CCTGTACACC TCCATGGCCC 3061 GCCCGCCCTT GCCTCGGCAG CAGTCTACCA GTTCAGACCG AGTGTCACAG ACGCCAGAGA 3121 GCCTGGATTT CCTCAAGGTC CCGGACCAGG GAGCTGCAGG GGTCAGGAGA CAAACAACCA 3181 GTCGGCCTcC CCCAGCAGGG GGCAGACCCA AGCCCCAGCC CAAGCCCAAG CCTCAGGTGC 3241 CACAGTGCAA GGCTTTGTAT GCCTATGACG CTCAGGACAC AGACGAACTC AGCTTTAATG 3301 CCAATGACAT TATTGATATT ATCAAAGAAG ATCCTTCTGG CTGGTGGACG GGTCGACTAC 3361 GAGGCAAGCA GGGCCTGTTC CCCAACAACT ATGTGACCAA GATCTTGCCA ACTTTCTTGT 3421 ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c