Construct: ORF ccsbBroadEn_14713

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004088.1_s300c1, BRDN0000391275
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NFIX (4784)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4784 NFIX nuclear factor I X NM_001365985.2 99.6% 99.5% 779T>C;783G>A;1297_1299delTCGinsGGT
2 human 4784 NFIX nuclear factor I X NM_002501.4 98.7% 98.1% (many diffs)
3 human 4784 NFIX nuclear factor I X NM_001271044.3 97.8% 97.7% (many diffs)
4 human 4784 NFIX nuclear factor I X NM_001365982.2 89.5% 88.8% (many diffs)
5 human 4784 NFIX nuclear factor I X NM_001365983.2 89.1% 89% (many diffs)
6 human 4784 NFIX nuclear factor I X NM_001365984.2 87.4% 82.5% (many diffs)
7 human 4784 NFIX nuclear factor I X NM_001365902.2 86.8% 81.4% (many diffs)
8 human 4784 NFIX nuclear factor I X XM_005259920.3 85.9% 80.9% (many diffs)
9 human 4784 NFIX nuclear factor I X NM_001271043.2 85.5% 79.5% (many diffs)
10 human 4784 NFIX nuclear factor I X XM_011528040.1 84.2% 78.5% (many diffs)
11 human 4784 NFIX nuclear factor I X XM_006722760.2 79% 77.8% (many diffs)
12 human 4784 NFIX nuclear factor I X XM_005259917.4 70.4% 65.2% (many diffs)
13 mouse 18032 Nfix nuclear factor I/X NM_001081982.2 93.9% 97.9% (many diffs)
14 mouse 18032 Nfix nuclear factor I/X NM_010906.3 84.9% 88.6% (many diffs)
15 mouse 18032 Nfix nuclear factor I/X NM_001297601.1 83.8% 88.1% (many diffs)
16 mouse 18032 Nfix nuclear factor I/X XM_006530761.3 82.5% 81.2% (many diffs)
17 mouse 18032 Nfix nuclear factor I/X XM_006530762.3 82.2% 80.7% (many diffs)
18 mouse 18032 Nfix nuclear factor I/X NM_001081981.2 81.7% 80.7% (many diffs)
19 mouse 18032 Nfix nuclear factor I/X XM_006530763.3 81.5% 79.7% (many diffs)
20 mouse 18032 Nfix nuclear factor I/X XM_006530759.3 70.7% 73.9% (many diffs)
21 mouse 18032 Nfix nuclear factor I/X XM_006530755.3 64.1% 63.3% (many diffs)
22 mouse 18032 Nfix nuclear factor I/X XM_006530760.3 64% 66.8% (many diffs)
23 mouse 18032 Nfix nuclear factor I/X XM_006530756.3 64% 63.3% (many diffs)
24 mouse 18032 Nfix nuclear factor I/X XM_011248326.2 63.8% 66.8% (many diffs)
25 mouse 18032 Nfix nuclear factor I/X XM_006530757.3 57.9% 57.1% (many diffs)
26 mouse 18032 Nfix nuclear factor I/X XM_006530758.3 57.8% 57.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1389
ORF length:
1320
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCTCCCGGCT TGCCGCCTGC AGGATGAGTT CCACCCGTTC ATCGAGGCAC
121TGCTGCCTCA CGTCCGCGCT TTCTCCTACA CCTGGTTCAA CCTGCAGGCG CGGAAGCGCA
181AGTACTTCAA GAAGCATGAA AAGCGGATGT CGAAGGACGA GGAGCGGGCG GTGAAGGACG
241AGCTGCTGGG CGAGAAGCCC GAGATCAAGC AGAAGTGGGC ATCCCGGCTG CTGGCCAAGC
301TGCGCAAGGA CATCCGGCCC GAGTTCCGCG AGGACTTCGT GCTGACCATC ACGGGCAAGA
361AGCCCCCCTG CTGCGTGCTC TCCAACCCCG ACCAGAAGGG CAAGATCCGG CGGATTGACT
421GCCTGCGCCA GGCTGACAAG GTGTGGCGGC TGGACCTGGT CATGGTGATT TTGTTTAAGG
481GGATCCCCCT GGAAAGTACT GATGGGGAGC GGCTCTACAA GTCGCCTCAG TGCTCGAACC
541CCGGCCTGTG CGTCCAGCCA CATCACATTG GAGTCACAAT CAAAGAACTG GATCTTTATC
601TGGCTTACTT TGTCCACACT CCGGAATCCG GACAATCAGA TAGTTCAAAC CAGCAAGGAG
661ATGCGGACAT CAAACCACTG CCCAACGGGC ACTTAAGTTT CCAGGACTGT TTTGTGACTT
721CCGGGGTCTG GAATGTGACG GAGCTGGTGA GAGTATCACA GACTCCTGTT GCAACAGCAT
781CAGGGCCCAA CTTCTCCCTG GCGGACCTGG AGAGTCCCAG CTACTACAAC ATCAACCAGG
841TGACCCCGGG ACGGCggtcc atcacctccc ctccttccac cagcaccacc aagcgcccca
901agtccatcga tgacagtgag atggagagcc ctgttgatga cgtgttctat cccgggacag
961gccgttcccc agcagctggc agcagccagt ccagcgggtg gcccaacgat gtggatgcag
1021gcccggcttc tctaaagaag tcaggaaagc tggacttctg cagtgccctc tcctctcagg
1081gcagctcccc gcgcatggct ttcacccacc acccgctgcc tgtgcttgct ggagtcagac
1141cagggagccc ccgggccaca gcatcagccc tgcacttccc ctccacgtcc atcatccagc
1201agtcgagccc gtatttcacg cacccgacca tccgctacca ccaccaccac gggcaggact
1261cactgaagga gtttgtgcag tttgtgtgct cggatggctc gggccaggcc accggacagc
1321attcgcaacg acaggcgcct cctctgccaa ccggtttgtc agcAGGTGAC CCCGGGACGG
1381CAACTTTCTT GCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
1441tttgttgcaa cgaac

Download FASTA (ORF) (Full)