Construct: ORF ccsbBroadEn_14727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012649.1_s300c1, BRDN0000458387
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK2 (5062)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | NM_002577.4 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | XM_011512870.2 | 99.7% | 99.8% | (many diffs) |
| 3 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | XM_017006501.1 | 99.7% | 99.8% | (many diffs) |
| 4 | human | 646214 | LOC646214 | p21 protein (Cdc42/Rac)-act... | NR_027053.2 | 18.2% | (many diffs) | |
| 5 | mouse | 224105 | Pak2 | p21 protein (Cdc42/Rac)-act... | NM_177326.3 | 89.5% | 96.9% | (many diffs) |
| 6 | mouse | 224105 | Pak2 | p21 protein (Cdc42/Rac)-act... | XM_006522072.3 | 89.5% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1638
- ORF length:
- 1572
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgtc tgataacgga gaactggaag ataagcctcc agcacctcct gtgcgaatga 121 gcagcaccat ctttagcact ggaggcaaag accctttgtc agccaatcac agtttgaaac 181 ctttgccctc tgttccagaa gagaaaaagc ccaggcataa aatcatctcc atattctcag 241 gcacagagaa aggaagtaaa aagaaggaaa aggaacggcc agaaatttct cctccatctg 301 attttgagca caccatccat gttggttttg atgctgttac tggagaattc actggcatgc 361 cagaacagtg ggctcgatta ctacagacct ccaatatcac caaactagag caaaagaaga 421 accctcaggc tgtgctggat gtcctaaagt tctacgactc caacacagtg aagcagaaat 481 atctgagctt tactcctcct gagaaagatg gctttccttc tggaacacca gcactgaatg 541 ccaagggaac agaagcaccc gcagtagtga cagaggagga ggatgatgat gaagagactg 601 ctcctcccgt tattgccccg cgaccggatc atacgaaatc aatttacaca cggtctgtaa 661 ttgaccctgt tcctgcacca gttggtgatt cacatgttga tggtgctgcc aagtctttag 721 acaaacagaa aaagaagact aagatgacag atgaagagat tatggagaaa ttaagaacta 781 tcgtgagcat aggtgaccct aagaaaaaat atacaagata tgaaaaaatt ggacaaaggg 841 cttctggtac agttttcact gctactgacg ttgcactggg acaggaggtt gctatcaaac 901 aaattaattt acagaaacag ccaaagaagg aactgatcat taacgagatt ctggtgatga 961 aagaattgaa aaatcccaac atcgttaact ttttggacag ttacctggta ggagatgaat 1021 tgtttgtggt catggaatac cttgctgggg ggtcactcac tgatgtggta acagaaacgt 1081 gcatggatga agcacagatt gctgctgtat gcagagagtg tttacaggca ttggagtttt 1141 tacatgctaa tcaagtgatc cacagagaca tcaaaagtga caatgtactt ttgggaatgg 1201 aaggatctgt taagctcact gactttggtt tctgtgccca gatcacccct gagcagagca 1261 aacgcagtac catggtcgga acgccatact ggatggcacc agaggtggtt acacggaaag 1321 cttatggccc taaagtcgac atatggtctc tgggtatcat ggctattgag atggtagaag 1381 gagagcctcc atacctcaat gaaaatccct tgagggcctt gtacctaata gcaactaatg 1441 gaaccccaga acttcagaat ccagagaaac tttccccaat atttcgggat ttcttaaatc 1501 gatgtttgga aatggatgtg gaaaaaaggg gttcagccaa agaattatta cagcatcctt 1561 tcctgaaact ggccaaaccg ttatctagct tgacaccact gatcatggca gctaaagaag 1621 caatgaagag taaccgttac ccaactttct tgtacaaagt tggcattata agaaagcatt 1681 gcttatcaat ttgttgcaac gaac