Construct: ORF ccsbBroadEn_14745

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001714.2_s300c1, BRDN0000458461
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKL (5211)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5211 PFKL phosphofructokinase, liver ... NM_001002021.3 99.5% 99.6% 1_9delATGAGAAGC;516C>T
2 human 5211 PFKL phosphofructokinase, liver ... XM_024452085.1 99.5% 99.6% 1_9delATGAGAAGC;516C>T
3 human 5211 PFKL phosphofructokinase, liver ... XM_006724011.1 96.9% 96.9% 1_9delATGAGAAGC;309_377del;585C>T
4 human 5211 PFKL phosphofructokinase, liver ... XM_017028368.1 96.9% 96.9% 1_9delATGAGAAGC;309_377del;585C>T
5 human 5211 PFKL phosphofructokinase, liver ... NM_002626.6 92.5% 91.7% (many diffs)
6 human 5211 PFKL phosphofructokinase, liver ... XM_017028369.1 91.4% 91.5% 0_1ins210;297C>T
7 human 5211 PFKL phosphofructokinase, liver ... XM_005261137.4 90.7% 90.8% 0_1ins228;279C>T
8 human 5211 PFKL phosphofructokinase, liver ... XM_017028370.1 90.7% 90.8% 0_1ins228;279C>T
9 human 5211 PFKL phosphofructokinase, liver ... XM_017028372.1 90.7% 90.8% 0_1ins228;279C>T
10 human 5211 PFKL phosphofructokinase, liver ... XM_005261135.3 90% 89.2% (many diffs)
11 human 5211 PFKL phosphofructokinase, liver ... XM_011529603.2 66.3% 64.1% (many diffs)
12 mouse 18641 Pfkl phosphofructokinase, liver,... NM_008826.4 81.5% 85.8% (many diffs)
13 mouse 18641 Pfkl phosphofructokinase, liver,... XM_017313846.1 49.2% 52.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2547
ORF length:
2481
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgtg taaccagggt agaggtcgag agtcctctcg tgggggtctc catgttcaag
121ggagctgccg aggcttgagc aggagccccc agcaggaaac tggctttgcc aaggcccccg
181ctgggacaga ctgtttcttt cactgcagtc ctgggagccg agggcaaggg gacaggaaag
241aggaagtgac ctcagagcct ggtggcacca gcatcatgtc caggctgggg ggcatgaacg
301ctgctgtccg ggctgtgacg cgcatgggca tttatgtggg tgccaaagtc ttcctcatct
361acgagggcta tgagggcctc gtggagggag gtgagaacat caagcaggcc aactggctga
421gcgtctccaa catcatccag ctgggcggca ctatcattgg cagcgctcgc tgcaaggcct
481ttaccaccag ggaggggcgc cgggcagcgg cctacaacct ggtccagcac ggcatcacca
541acctgtgcgt catcggcggg gatggcagcc ttacaggtgc caacatcttc cgcagcgagt
601ggggcagcct gctggaggag ctggtggcgg aaggtaagat ctcagagact acagcccgga
661cctactcgca cctgaacatc gcgggcctag tgggctccat cgataacgac ttctgcggca
721ccgacatgac catcggcacg gactcggccc tccaccgcat catggaggtc atcgatgcca
781tcaccaccac tgcccagagc caccagagga ccttcgtgct ggaagtgatg ggccggcact
841gcgggtacct ggcgctggta tctgcactgg cctcaggggc cgactggctg ttcatccccg
901aggctccacc cgaggacggc tgggagaact tcatgtgtga gaggctgggt gagactcgga
961gccgtgggtc ccgactgaac atcatcatca tcgctgaggg tgccattgac cgcaacggga
1021agcccatctc gtccagctac gtgaaggacc tggtggttca gaggctgggc ttcgacaccc
1081gtgtaactgt gctgggccac gtgcagcggg gagggacgcc ctctgccttc gaccggatcc
1141tgagcagcaa gatgggcatg gaggcggtga tggcgctgct ggaagccacg cctgacacgc
1201cggcctgcgt ggtcaccctc tcggggaacc agtcagtgcg gctgcccctc atggagtgcg
1261tgcagatgac caaggaagtg cagaaagcca tggatgacaa gaggtttgac gaggccaccc
1321agctccgtgg tgggagcttc gagaacaact ggaacattta caagctcctc gcccaccaga
1381agccccccaa ggagaagtct aacttctccc tggccatcct gaatgtgggg gccccggcgg
1441ctggcatgaa tgcggccgtg cgctcggcgg tgcggaccgg catctcccat ggacacacag
1501tatacgtggt gcacgatggc ttcgaaggcc tagccaaggg tcaggtgcaa gaagtaggct
1561ggcacgacgt ggccggctgg ttggggcgtg gtggctccat gctggggacc aagaggaccc
1621tgcccaaggg ccagctggag tccattgtgg agaacatccg catctatggt attcacgccc
1681tgctggtggt cggtgggttt gaggcctatg aaggggtgct gcagctggtg gaggctcgcg
1741ggcgctacga ggagctctgc atcgtcatgt gtgtcatccc agccaccatc agcaacaacg
1801tccctggcac cgacttcagc ctgggctccg acactgctgt aaatgccgcc atggagagct
1861gtgaccgcat caaacagtct gcctcgggga ccaagcgccg tgtgttcatc gtggagacca
1921tggggggtta ctgtggctac ctggccaccg tgactggcat tgctgtgggg gccgacgccg
1981cctacgtctt cgaggaccct ttcaacatcc acgacttaaa ggtcaacgtg gagcacatga
2041cggagaagat gaagacagac attcagaggg gcctggtgct gcggaacgag aagtgccatg
2101actactacac cacggagttc ctgtacaacc tgtactcatc agagggcaag ggcgtcttcg
2161actgcaggac caatgtcctg ggccacctgc agcagggtgg cgctccaacc ccctttgacc
2221ggaactatgg gaccaagctg ggggtgaagg ccatgctgtg gttgtcggag aagctgcgcg
2281aggtttaccg caagggacgg gtgttcgcca atgccccaga ctcggcctgc gtgatcggcc
2341tgaagaagaa ggcggtggcc ttcagccccg tcactgagct caagaaagac actgatttcg
2401agcaccgcat gccacgggag cagtggtggc tgagcctgcg gctcatgctg aagatgctgg
2461cacaataccg catcagtatg gccgcctacg tgtcagggga gctggagcac gtgacccgcc
2521gcaccctgag catggacaag ggcttctgcc caactttctt gtacaaagtt ggcattataa
2581gaaagcattg cttatcaatt tgttgcaacg aac

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