Construct: ORF ccsbBroadEn_14752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018962.1_s300c1, BRDN0000458258
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHKA2 (5256)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5256 | PHKA2 | phosphorylase kinase regula... | NM_000292.3 | 99.4% | 19.7% | (many diffs) |
| 2 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_006724496.4 | 98.7% | 19.6% | (many diffs) |
| 3 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_005274548.5 | 97.9% | 20% | (many diffs) |
| 4 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_005274550.5 | 97.1% | 20.2% | (many diffs) |
| 5 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_011545537.3 | 96.1% | 17.4% | (many diffs) |
| 6 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_006724498.4 | 84.1% | 4.9% | (many diffs) |
| 7 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_002958777.1 | 83.5% | (many diffs) | |
| 8 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_001755697.2 | 81.6% | (many diffs) | |
| 9 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_017029580.2 | 74.5% | 5.6% | (many diffs) |
| 10 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_950461.3 | 72.1% | (many diffs) | |
| 11 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_011545538.3 | 71.3% | 5.9% | (many diffs) |
| 12 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_001755698.2 | 52.4% | (many diffs) | |
| 13 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_001177879.1 | 86.8% | 19.4% | (many diffs) |
| 14 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_172783.3 | 86.8% | 19.4% | (many diffs) |
| 15 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528679.3 | 86.4% | 19.3% | (many diffs) |
| 16 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528680.3 | 84.8% | 19.7% | (many diffs) |
| 17 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_001177878.1 | 83.4% | 20.1% | (many diffs) |
| 18 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528681.1 | 82.6% | 14.8% | (many diffs) |
| 19 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_387078.3 | 75.9% | (many diffs) | |
| 20 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528682.1 | 73.5% | 4.9% | (many diffs) |
| 21 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_001782730.1 | 70.5% | (many diffs) | |
| 22 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_001782731.1 | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 100
- ORF end:
- 859
- ORF length:
- 759
- Sequence:
-
1 GAAGGGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TGAGCAATGC 61 TTTTTTATAA TGCCAACTTT GTACAAAAAA GTTGGCACCA TGCGGAGCAG GAGCAATTCC 121 GGGGTCCGCT TGGACGGGTA CGCGCGGCTG GTGCAGCAAA CCATCCTGTG TTACCAGAAT 181 CCCGTCACGG GGCTGCTGTC AGCCAGCCAT GAGCAGAAGG ATGCCTGGGT GCGGGATAAC 241 ATCTACAGTA TCCTGGCCGT GTGGGGCCTG GGCATGGCCT ACCGTAAGAA TGCAGACCGC 301 GATGAGGACA AGGCCAAGGC CTACGAGCTG GAGCAGAACG TGGTGAAGCT GATGCGAGGT 361 CTTCTCCAGT GCATGATGAG ACAGGTGGCC AAAGTGGAGA AGTTCAAACA CACTCAGAGC 421 ACCAAGGACA GCCTGCACGC CAAGTACAAC ACCGCCACCT GTGGCACGGT GGTGGGCGAC 481 GACCAGTGGG GCCACCTCCA GGTGGATGCC ACCTCTCTCT TCCTCCTGTT CCTGGCCCAG 541 ATGACCGCCT CAGGCTTACG TATCATTTTC ACTCTCGATG AGGTGGCCTT CATACAGAAT 601 CTTGTCTTTT ACATAGAAGC TGCATATAAA GTCGCTGATT ATGGAATGTG GGAGCGTGGA 661 GATAAGACTA ATCAGGGCAT CCCGGAATTG AATGCAAGCT CCGTAGGAAT GGCCAAGGCA 721 GCTCTTGAGG CAATTGATGA ACTGGACCTT TTTGGAGCCC ATGGAGGACG CAAGTCAGTG 781 ATTCATGTTC TGCCAGATGA GGTCGAGCAC TGCCAGTCTT ATTCTTGTTC TCCATGCTGC 841 CCAAGAAGCG TCgacatcta aagaaattga tgctggactt ctttccatta tttccttccc 901 ggcctttgca gtggaagatg taaaccttgt aaatgtgacc aaaaatgaaa ttatttctaa 961 gctccagggg cgttatggat gctgtcgctt ccttcgagat ggttataaaa ctccaagaga 1021 ggaccctaat cgactgcatt atgaccctgc tgaactcaag ctcttcgaaa acattgaatg 1081 tgagtggcct gtgttttgga catattttat aatagatgga gtcttcagtg gtgatgctgt 1141 tcaggtccaa gaataccgag aggccctgga gggaatactc atcagaggca agaatgggat 1201 ccgcctggtg cctgaactct acgctgtccc gcctaacaag gtagatgaag agtacaagaa 1261 tcctcacaca gtagaccgag ttcctatggg gaaggtgcct catctgtggg gccaatcctt 1321 gtacatcctc agctcgctgt tggcagaggg attccttgcc gctggtgaaa tcgatccctt 1381 aaatagaaga ttttccactt cagtcaaacc tgatgttgta gtacaagtta ctgttttggc 1441 agaaaacaat cacattaagg acttattgag gaaacacggg gtgaacgtcc agagtatcgc 1501 ggacattcat ccaattcaag tccagccggg ccggattctt agtcacatat atgccaagct 1561 tggacggaat aagaatatga atttgagtgg gcgaccgtat cgacatattg gtgtccttgg 1621 aacctctaaa ctatatgtga ttaggaacca aatctttact tttacacccc agttcaccga 1681 cgagcatcac ttctacctgg ccctcgacaa tgagatgatc gtggagatgc taaggatcga 1741 gctggcctac ctgtgcacct gctggaggat gacgggcaga cccacactca ccttccccat 1801 cagtcgcacc atgctcacaa atgatggctc agacattcat tctgctgtgc tctccacaat 1861 tagaaaacta gaggatggat attttggagg agccagagta aaattaggga acctttcgga 1921 atttctcacc acatcgttct acacatatct gacttttctg gatccagact gtgatgagaa 1981 gttgtttgac aatgccagcg aagggacttt cagtcctgat agtgattcag atttggtagg 2041 atatctggaa gacacctgta atcaagaaag ccaagacgaa cttgaccatt atatcaacca 2101 ccttctgcaa agcacatcgt tgaggtccta tctgcctcct ctttgtaaga acacagaaga 2161 ccgccatgtc ttcagtgcta tccactccac gcgggacata ctttctgtga tggcaaaagc 2221 aaagggtttg gaagttccat ttgttcccat gactttgccg actaaagttc taagtgccca 2281 ccgtaaatca ctgaatcttg ttgattctcc tcagccactc ctagaaaagg ttcctgaaag 2341 tgactttcag tggcccagag atgaccatag tgacgtggac tgtgagaagc tggttgagca 2401 gctaaaagat tgttcgaacc tacaggacca agcagacatt ctgtacattc tttatgtcat 2461 aaagggtccc agctgggaca caaatctctc tggacagcac ggggtcaccg ttcaaaacct 2521 tcttggtgag ctctatggga aagccggctt gaaccaggag tggggtctga ttcgctacat 2581 ctcaggcctt ctcaggaaga aagtggaggt cctggctgag gcctgcacag acctgctttc 2641 gcaccagaag cagctcaccg tgggcctgcc gcccgagccc cgggagaaga tcatctctgc 2701 gccccttccc ccagaggagc tcacaaaact catctacgag gccagtgggc aggacatcag 2761 cattgccgtc ctcacgcagg agattgtggt ttacctggcc atgtatgtca gggcgcagcc 2821 cagcctcttt gtggagatgc tgagactccg gattggactg atcattcagg tgatggccac 2881 ggagctggca cggagcctga actgctcagg agaagaggct tctgaaagtt tgatgaacct 2941 cagccctttc gatatgaaaa atctcctgca ccatattcta agtgggaaag agtttggcgt 3001 tgaaagaagt gtgcgcccta tCCACTCTCA CAATCAGCCC TACATCTCAT CACGAGGTGT 3061 CATACGGAGT CACAAAACTG AGAGGAGTGG CATTACAGAC TGAGAGTGAA ATGAAACAGA 3121 TGACTAGGCG GTTTAGTGCT GATGAACAGT TCTTTTCTGT GGGCCAGGCC GCGTCCAGCA 3181 GTGCGCATTC CTCCAAGTCT GCGAGGTCCA GCACCCCATC CTCGCCCACT GGCACGTCAT 3241 CCTCAGACTC GGGAGGACAT CACATCGGCT GGGGTGAGCG GCAGGGCCAG TGGCTGCGCA 3301 GGAGAAGGCT GGATGGGGCC ATCAACAGGG TCCCCGTGGG ATTCTACCAG AGGGTGTGGA 3361 AGATCCTCCA GAAGTGCCAC GGTCTCTCCA TCGATGGTTA TGTCCTCCCA TCCTCGACGA 3421 CCCGAGAGAT GACCCCGCAT GAGATCAAGT TTGCTGTCCA TGTCGAATCG GTGCTGAACC 3481 GCGTGCCGCA GCCCGAGTAC CGGCAGCTGC TGGTGGAAGC CATCATGGTG CTGACGCTGC 3541 TCTCGGACAC GGAGATGACC AGCATCGGGG GCATCATCCA CGTGGACCAG ATCGTGCAGA 3601 TGGCCAGTCA GCTGTTCTTG CAGGACCAGG TGTCAATTGG TGCCATGGAC ACCCTGGAGA 3661 AAGACCAAGC CACAGGAATC TGCCACTTCT TTTATGACAG CGCTCCGAGT GGGGCTTATG 3721 GGACGATGAC CTACCTAACA AGAGCAGTGG CTTCTTATTT GCAGGAATTG TTGCCCAATT 3781 CGGGCTGCCA GATGCAATTG CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT 3841 GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA 3901 TCCAGCTGTT CAGAGG