Construct: ORF ccsbBroadEn_14776

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001629.2_s300c1, BRDN0000458603
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAA1 (5562)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5562 PRKAA1 protein kinase AMP-activate... NM_006251.5 98.3% 98.3% 1_27del
2 human 5562 PRKAA1 protein kinase AMP-activate... NM_206907.3 95.8% 95.8% 1_27del;363_407del
3 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355028.2 95.7% 93.3% (many diffs)
4 human 5562 PRKAA1 protein kinase AMP-activate... XM_017009625.2 95.7% 93.3% (many diffs)
5 human 5562 PRKAA1 protein kinase AMP-activate... XM_017009624.1 93.2% 90.8% (many diffs)
6 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355029.2 75.4% 75.4% 0_1ins405
7 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355035.2 54.7% 54.7% 0_1ins747
8 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355036.2 54.7% 54.7% 0_1ins747
9 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355037.2 54.7% 54.7% 0_1ins747
10 human 5562 PRKAA1 protein kinase AMP-activate... NM_001355034.1 35.8% 33.3% (many diffs)
11 mouse 105787 Prkaa1 protein kinase, AMP-activat... NM_001013367.3 88.6% 97.1% (many diffs)
12 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_011245321.2 74.9% 82% (many diffs)
13 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_017316356.1 74.9% 82% (many diffs)
14 mouse 105787 Prkaa1 protein kinase, AMP-activat... XM_006519953.2 67.6% 74.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1716
ORF length:
1650
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggc gacagccgag aagcagaaac acgacgggcg ggtgaagatc ggccactaca
121ttctgggtga cacgctgggg gtcggcacct tcggcaaagt gaaggttggc aaacatgaat
181tgactgggca taaagtagct gtgaagatac tcaatcgaca gaagattcgg agccttgatg
241tggtaggaaa aatccgcaga gaaattcaga acctcaagct tttcaggcat cctcatataa
301ttaaactgta ccaggtcatc agtacaccat ctgatatttt catggtgatg gaatatgtct
361caggaggaga gctatttgat tatatctgta agaatggaag gctggatgaa aaagaaagtc
421ggcgtctgtt ccaacagatc ctttctggtg tggattattg tcacaggcat atggtggtcc
481atagagattt gaaacctgaa aatgtcctgc ttgatgcaca catgaatgca aagatagctg
541attttggtct ttcaaacatg atgtcagatg gtgaattttt aagaacaagt tgtggctcac
601ccaactatgc tgcaccagaa gtaatttcag gaagattgta tgcaggccca gaggtagata
661tatggagcag tggggttatt ctctatgctt tattatgtgg aacccttcca tttgatgatg
721accatgtgcc aactcttttt aagaagatat gtgatgggat cttctatacc cctcaatatt
781taaatccttc tgtgattagc cttttgaaac atatgctgca ggtggatccc atgaagaggg
841ccacaatcaa agatatcagg gaacatgaat ggtttaaaca ggaccttcca aaatatctct
901ttcctgagga tccatcatat agttcaacca tgattgatga tgaagcctta aaagaagtat
961gtgaaaagtt tgagtgctca gaagaggaag ttctcagctg tctttacaac agaaatcacc
1021aggatccttt ggcagttgcc taccatctca taatagataa caggagaata atgaatgaag
1081ccaaagattt ctatttggcg acaagcccac ctgattcttt tcttgatgat catcacctga
1141ctcggcccca tcctgaaaga gtaccattct tggttgctga aacaccaagg gcacgccata
1201cccttgatga attaaatcca cagaaatcca aacaccaagg tgtaaggaaa gcaaaatggc
1261atttaggaat tagaagtcaa agtcgaccaa atgatattat ggcagaagta tgtagagcaa
1321tcaaacaatt ggattatgaa tggaaggttg taaacccata ttatttgcgt gtacgaagga
1381agaatcctgt gacaagcact tactccaaaa tgagtctaca gttataccaa gtggatagta
1441gaacttatct actggatttc cgtagtattg atgatgaaat tacagaagcc aaatcaggga
1501ctgctactcc acagagatcg ggatcagtta gcaactatcg atcttgccaa aggagtgatt
1561cagatgctga ggctcaagga aaatcctcag aagtttctct tacctcatct gtgacctcac
1621ttgactcttc tcctgttgac ctaactccaa gacctggaag tcacacaata gaattttttg
1681agatgtgtgc aaatctaatt aaaattcttg cacaatnncc aactttcttg tacaaagttg
1741gcattataag aaagcattgc ttatcaattt gttgcaacga ac

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