Construct: ORF ccsbBroadEn_14785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018812.1_s300c1, BRDN0000458108
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKAR1B (5575)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164758.1 | 99.3% | 33.1% | (many diffs) |
| 2 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164759.1 | 99.3% | 33.1% | (many diffs) |
| 3 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164760.2 | 99.3% | 33.1% | (many diffs) |
| 4 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164761.1 | 99.3% | 33.1% | (many diffs) |
| 5 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164762.1 | 99.3% | 33.1% | (many diffs) |
| 6 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_002735.2 | 99.3% | 33.1% | (many diffs) |
| 7 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_011515446.2 | 84.9% | 18% | (many diffs) |
| 8 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_017012433.1 | 61.8% | 4.2% | (many diffs) |
| 9 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_001253890.1 | 85.3% | 28.7% | (many diffs) |
| 10 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_008923.3 | 85.3% | 28.7% | (many diffs) |
| 11 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504660.3 | 85.3% | 28.7% | (many diffs) |
| 12 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504661.3 | 85.3% | 28.7% | (many diffs) |
| 13 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504663.2 | 85.3% | 28.7% | (many diffs) |
| 14 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_017320719.1 | 85.3% | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 510
- ORF length:
- 429
- Sequence:
-
1 TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT 61 TGTACAAAAA AGTTGGCACC ATGGCCTCCC CGCCCGCCTG CCCCTCGGAG GAGGACGAGA 121 GCCTGAAGGG CTGTGAGCTG TACGTGCAGC TGCACGGGAT CCAGCAGGTC CTCAAAGACT 181 GTATCGTCCA CCTCTGCATC TCCAAGCCCG AACGCCCCAT GAAGTTCCTC CGGGAGCACT 241 TCGAGAAGCT GGAGAAGGAA GAAAACAGGC AGATTTTGGC GCGGCAAAAG TCAAACTCAC 301 AGTCGGACTC CCATGATGAG GAGGTGTCGC CCACCCCCCC GAACCCTGTG GTGAAGGCCC 361 GCCGCCGGCG AGGAGGCGTG AGTGCCGAGG TGTACACCGA GGAGGACGCC GNGTCCTACG 421 TCAGGAAAGG TGATTCCCCA AGGACTACAA AAACCATGAC TGCGCTGGCC CAAGGCCATC 481 TCCAAGAACG TGCTCTTCGC TCACCTGGAT GACAACGAGA GGAGTGACAT ATTCGATGCC 541 ATGTTCCCTG TCACTCACAT CGCTGGGGAG ACTGTTATAC AGCAAGGGAA TGAAGGAGAC 601 AACTTCTATG TCGTTGATCA AGGGGAAGTG GATGTGTACG TGAACGGAGA GTGGGTGACC 661 AACATCAGCG AGGGAGGCAG CTTCGGGGAG CTGGCGCTCA TCTACGGCAC CCCCAGGGCT 721 GCGACCGTGA AAGCCAAGAC GGACCTCAAG CTCTGGGGGA TCGACCGGGA CAGCTACCGG 781 CGCATCCTTA TGGGCAGCAC GCTGAGGAAA CGCAAGATGT ACGAGGAGTT CCTCAGCAAG 841 GTCTCCATCC TAGAGTCCCT GGGAGAAGTG GGAGCGTCTG ACCGTGGCGG ATGCGCTGGG 901 AGCCCGTCCA GTTTGAAGAT GGAGAGAAAA TTGTGGTCCA GGGAGAGCCT GGGGACGACT 961 TTTACATCAT CACGGAGGGC ACCGCGTCCG TGCTGCAGCG CCGGTCCCCC AATGAGGAGT 1021 ACGTGGAGGT GGGGCGCCTG GGACCCTCTG ACTACTTCGG GGAGATTGCA CTGCTGCTGA 1081 ACCGGCCCCG GGCGGCCACT GTCGTGGCCC GGGGGCCCCT CAAGTGTGTG AAGCTGGACC 1141 GGCCCCGCTT CGAGCGTGTG CTGGGGCCCT GCTCTGAGAT CCTCAAGAGG AACATTCAGC 1201 GTTACAACAG CTTCATCTCC CTCACCGTCT TGCCAACTTT CTTGTACAAA GTTGGCATTA 1261 TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA 1321 TCATTATTGC CATCCAGCGA TCAGAG