Transcript: Human NM_002735.2

Homo sapiens protein kinase cAMP-dependent type I regulatory subunit beta (PRKAR1B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
PRKAR1B (5575)
Length:
2512
CDS:
134..1279

Additional Resources:

NCBI RefSeq record:
NM_002735.2
NBCI Gene record:
PRKAR1B (5575)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146606 GGTGTACGTGAACGGAGAGT pXPR_003 GGG 565 49% 7 0.7442 PRKAR1B PRKAR1B 76838
2 BRDN0001144765 GAGTGCCGAGGTGTACACCG pXPR_003 AGG 316 28% 3 0.6668 PRKAR1B PRKAR1B 76839
3 BRDN0001147089 GAGGAAACGCAAGATGTACG pXPR_003 AGG 736 64% 8 0.4314 PRKAR1B PRKAR1B 76840
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002735.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052563 GACAACTTCTATGTCGTTGAT pLKO.1 647 CDS 100% 4.950 6.930 N PRKAR1B n/a
2 TRCN0000052564 GAGTGACATATTCGATGCCAT pLKO.1 571 CDS 100% 2.640 3.696 N PRKAR1B n/a
3 TRCN0000195302 CAACGAGAGGAGTGACATATT pLKO.1 562 CDS 100% 13.200 9.240 N PRKAR1B n/a
4 TRCN0000195143 CCTTGTGCATTTCCAAATCAA pLKO.1 1448 3UTR 100% 5.625 3.938 N PRKAR1B n/a
5 TRCN0000052565 GAGATCCTCAAGAGGAACATT pLKO.1 1223 CDS 100% 5.625 3.938 N PRKAR1B n/a
6 TRCN0000082635 CCAGTTTGAAGATGGAGAGAA pLKO.1 955 CDS 100% 4.950 3.465 N LOC442370 n/a
7 TRCN0000024750 GCTGAGGAAACGCAAGATGTA pLKO.1 850 CDS 100% 4.950 3.465 N Prkar1b n/a
8 TRCN0000052567 TCAGCGTTACAACAGCTTCAT pLKO.1 1243 CDS 100% 4.950 3.465 N PRKAR1B n/a
9 TRCN0000052566 ACTGTTATACAGCAAGGGAAT pLKO.1 620 CDS 100% 4.050 2.835 N PRKAR1B n/a
10 TRCN0000082637 CATCCTAGAGTCCCTGGAGAA pLKO.1 895 CDS 100% 4.050 2.835 N LOC442370 n/a
11 TRCN0000199097 CCCTCAAGTGTGTGAAGCTGG pLKO.1 1164 CDS 100% 0.720 0.504 N PRKAR1B n/a
12 TRCN0000199098 CTGTGAGCTGTACGTGCAGCT pLKO.1 184 CDS 100% 0.072 0.050 N PRKAR1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002735.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000472795 CAGCGTCCGAGACTGGTGCCCCTT pLX_317 34.7% 99.5% 33.1% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14785 pDONR223 100% 99.3% 33.1% None (many diffs) n/a
3 ccsbBroad304_14785 pLX_304 0% 99.3% 33.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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