Transcript: Mouse XM_006504661.3

PREDICTED: Mus musculus protein kinase, cAMP dependent regulatory, type I beta (Prkar1b), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Prkar1b (19085)
Length:
2601
CDS:
103..1248

Additional Resources:

NCBI RefSeq record:
XM_006504661.3
NBCI Gene record:
Prkar1b (19085)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006504661.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024753 TGCGAGATGTACGTGCAGAAA pLKO.1 154 CDS 100% 4.950 6.930 N Prkar1b n/a
2 TRCN0000285724 TGGTCTAGGCCCAAGTGTAAA pLKO_005 1669 3UTR 100% 13.200 10.560 N Prkar1b n/a
3 TRCN0000274597 TTGGACCCTCTGACTACTTTG pLKO_005 1055 CDS 100% 10.800 8.640 N Prkar1b n/a
4 TRCN0000274647 CAAGGAGAAGTAGATGTATAT pLKO_005 637 CDS 100% 13.200 9.240 N Prkar1b n/a
5 TRCN0000024749 CCAGCGTTACAACAGCTTCAT pLKO.1 1212 CDS 100% 4.950 3.465 N Prkar1b n/a
6 TRCN0000274648 CCAGCGTTACAACAGCTTCAT pLKO_005 1212 CDS 100% 4.950 3.465 N Prkar1b n/a
7 TRCN0000024751 GCCTGGAGATGACTTCTACAT pLKO.1 963 CDS 100% 4.950 3.465 N Prkar1b n/a
8 TRCN0000274649 CCTACGTGAGGAAGGTCATTC pLKO_005 437 CDS 100% 10.800 6.480 N Prkar1b n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006504661.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000472795 CAGCGTCCGAGACTGGTGCCCCTT pLX_317 34.7% 85.5% 28.7% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14785 pDONR223 100% 85.3% 28.7% None (many diffs) n/a
3 ccsbBroad304_14785 pLX_304 0% 85.3% 28.7% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV