Construct: ORF ccsbBroadEn_14795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018860.1_s300c1, BRDN0000458156
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCQ (5588)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323265.1 | 99.8% | 31.5% | (many diffs) |
| 2 | human | 5588 | PRKCQ | protein kinase C theta | NM_006257.5 | 99.8% | 31.5% | (many diffs) |
| 3 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448076.1 | 99.8% | 31.5% | (many diffs) |
| 4 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252496.4 | 95.2% | 30.1% | (many diffs) |
| 5 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282644.2 | 94.7% | 25.9% | (many diffs) |
| 6 | human | 5588 | PRKCQ | protein kinase C theta | NM_001242413.2 | 90.8% | 34.6% | (many diffs) |
| 7 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252497.4 | 89% | 31.9% | (many diffs) |
| 8 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323267.1 | 88.2% | 27.6% | (many diffs) |
| 9 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282645.1 | 82.1% | 13.7% | (many diffs) |
| 10 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323266.2 | 82.1% | 13.7% | (many diffs) |
| 11 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448077.1 | 82.1% | 13.7% | (many diffs) |
| 12 | mouse | 18761 | Prkcq | protein kinase C, theta | NM_008859.2 | 87% | 30.8% | (many diffs) |
| 13 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497398.3 | 81.9% | 25% | (many diffs) |
| 14 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_011238965.2 | 72.6% | 13.4% | (many diffs) |
| 15 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497399.2 | 55.9% | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 773
- ORF length:
- 684
- Sequence:
-
1 TTTGGACTGG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT TTTTTATAAT 61 GCCAACTTTG TACAAAAAAG TTGGCACCAT GTCGCCATTT CTTCGGATTG GCTTGTCCAA 121 CTTTGACTGC GGGTCCTGCC AGTCTTGTCA GGGCGAGGCT GTTAACCCTT ACTGTGCTGT 181 GCTCGTCAAA GAGTATGTCG AATCAGAGAA CGGGCAGATG TATATCCAGA AAAAGCCTAC 241 CATGTACCCA CCCTGGGACA GCACTTTTGA TGCCCATATC AACAAGGGAA GAGTCATGCA 301 GATCATTGTG AAAGGCAAAA ACGTGGACCT CATCTCTGAA ACCACCGTGG AGCTCTACTC 361 GCTGGCTGAG AGGTGCAGGA AGAACAACGG GAAGACAGAA ATATGGTTAG AGCTGAAACC 421 TCAAGGCCGA ATGCTAATGA ATGCAAGATA CTTTCTGGAA ATGAGTGACA CAAAGGACAT 481 GAATGAATTT GAGACGGAAG GCTTCTTTGC TTTGCATCAG CGCCGGGGTG CCATCAAGCA 541 GGCAAAGGTC CACCACGTCA AGTGCCACGA GTTCACTGCC ACCTTCTTCC CACAGCCCAC 601 ATTTTGCTCT GTCTGCCACG AGTTTGTCTG GGGCCTGAAC AAACAGGGCT ACCAGTGCCG 661 ACAATGCAAT GCAGCAATTC ACAAGAAGTG TATTGATAAA GTTATAGCAA AGTGCACAGG 721 ATCAGCTATC AATAGCCGAG AAACCATGTT CCACAAAGGA GAGATTCAAA ATTGACATGC 781 CACACAGATT TAAAGTCTAC AATTACAAGA GCCCGACCTT CTGTGAACAC TGTGGGACCC 841 tgctgtgggg actggcacgg caaggactca agtgtgatgc atgtggcatg aatgtgcatc 901 atagatgcca gacaaaggtg gccaaccttt gtggcataaa ccagaagcta atggctgaag 961 cgctggccat gattgagagc actcaacagg ctcgctgctt aagagatact gaacagatct 1021 tcagagaagg tccggttgaa attggtctcc catgctccat caaaaatgaa gcaaggccgc 1081 catgtttacc gacaccggga aaaagagagc ctcagggcat ttcctgggag tctccgttgg 1141 atgaggtgga taaaatgtgc catcttccag aacctgaact gaacaaagaa agaccatctc 1201 tgcagattaa actaaaaatt gaggatttta tcttgcacaa aatgttgggg aaaggaagtt 1261 ttggcaaggt cttcctggca gaattcaaga aaaccaatca atttttcgca ataaaggcct 1321 taaagaaaga tgtggtcttg atggacgatg atgttgagtg cacgatggta gagaagagag 1381 ttctttcctt ggcctgggag catccgtttc tgacgcacat gttttgtaca ttccaGACCA 1441 AGGAAAACCT CTTTTTTTGT GATGGAGTAC CTCAACGGAG GGGACTTAAT GTACCACATC 1501 CAAAGCTGCC ACAAGTTCGA CCTTTCCAGA GCGACGTTTT ATGCTGCTGA AATCATTCTT 1561 GGTCTGCAGT TCCTTCATTC CAAAGGAATA GTCTACAGGG ACCTGAAGCT AGATAACATC 1621 CTGTTAGACA AAGATGGACA TATCAAGATT GCGGATTTTG GAATGTGCAA GGAGAACATG 1681 TTAGGAGATG CCAAGACGAA TACCTTCTGT GGGACACCTG ACTACATCGC CCCAGAGATC 1741 TTGCTGGGTC AGAAATACAA CCACTCTGTG GACTGGTGGT CCTTCGGGGT TCTCCTTTAT 1801 GAAATGCTGA TTGGTCAGTC GCCTTTCCAC GGGCAGGATG AGGAGGAGCT CTTCCACTCC 1861 ATCCGCATGG ACAATCCCTT TTACCCACGG TGGCTGGAGA AGGAAGCAAA GGACCTTCTG 1921 GTGAAGCTCT TCGTGAGAGA ACCTGAGAAG AGGCTGGGCG TGAGGGGAGA CATCCGCCAG 1981 CACCCTTTGT TTCGGGAGAT CAACTGGGAG GAACTTGAAC GGAAGGAGAT TGACCCACCG 2041 TTCCGGCCGA AAGTGAAATC ACCATTTGAC TGCAGCAATT TCGACAAAGA ATTCTTAAAC 2101 GAGAAGCCCC GGCTGTCATT TGCCGACAGA GCACTGATCA ACAGCATGGA CCAGAATATG 2161 TTCAGGAACT TTTCCTTCAT GAACCCCGGG ATGGAGCGGC TGATATCCTT GCCAACTTTC 2221 TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC 2281 ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATTTCCCGA