Construct: ORF ccsbBroadEn_14812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018888.1_s300c1, BRDN0000458184
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EIF2AK2 (5610)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135651.3 | 96.3% | 43.1% | (many diffs) |
| 2 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_002759.3 | 96.3% | 43.1% | (many diffs) |
| 3 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_011532987.2 | 96.3% | 43.1% | (many diffs) |
| 4 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135652.2 | 89.1% | 46.5% | 715_717delGCAinsC;785_786ins123;1357_1410del |
| 5 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_017004503.1 | 88.5% | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 837
- ORF length:
- 738
- Sequence:
-
1 ATATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCTGGTGAT CTTTCAGCAG 121 GTTTCTTCAT GGAGGAACTT AATACATACC GTCAGAAGCA GGGAGTAGTA CTTAAATATC 181 AAGAACTGCC TAATTCAGGA CCTCCACATG ATAGGAGGTT TACATTTCAA GTTATAATAG 241 ATGGAAGAGA ATTTCCAGAA GGTGAAGGTA GATCAAAGAA GGAAGCAAAA AATGCCGCAG 301 CCAAATTAGC TGTTGAGATA CTTAATAAGG AAAAGAAGGC AGTTAGTCCT TTATTATTGA 361 CAACAACGAA TTCTTCAGAA GGATTATCCA TGGGGAATTA CATAGGCCTT ATCAATAGAA 421 TTGCCCAGAA GAAAAGACTA ACTGTAAATT ATGAACAGTG TGCATCGGGG GTGCATGGGC 481 CAGAAGGATT TCATTATAAA TGCAAAATGG GACAGAAAGA ATATAGTATT GGTACAGGTT 541 CTACTAAACA GGAAGCAAAA CAATTGGCCG CTAAACTTGC ATATCTTCAG ATATTATCAG 601 AAGAAACCTC AGTGAAATCT GACTACCTGT CCTCTGGTTC TTTTGCTACT ACGTGTGAGT 661 CCCAAAGCAA CTCTTTAGTG ACCAGCACAC TCGCTTCTGA ATCATCATCT GAAGGTGACT 721 TCTCAGCAGA TACATCAGAG ATAAATTCTA ACAGTGACAG TTTAAACAGT TCTTCGTTGC 781 TTATGAATGG TCTCAGAAAT AATCAAAGGA AGCAAAAGAT CTTTGGCACC CAGATTTGAC 841 CTTCCTGACA TGAAAGAAAC AAAGTATACT GTGGAcaaga ggtttggcat ggattttaaa 901 gaaatagaat taattggctc aggtggattt ggccaagttt tcaaagcaaA ACACAGAATT 961 GACCGGAAGA CTTACGGTAT TAAACGTGTT AAATATAATA ACGAGAAGGC GGAGCGTGAA 1021 GTAAAAGCAT TGGCAAAACT TGATCATGTA AATATTGTTC ACTACAATGG CTGTTGGGAT 1081 GGATTTGATT ATGATCCTGA GACCAGTGAT GATTCTCTTG AGAGCAGTGA TTATGATCCT 1141 GAGAACAGCA AAAATAGTTC AAGGTCAAAG ACTAAGTGCC TTTTCATCCA AATGGAATTC 1201 TGTGATAAAG GGACCTTGGA ACAATGGATT GAAAAAAGAA GAGGCGAGAA ACTAGACAAA 1261 GTTTTGGCTT TGGAACTCTT TGAACAAATA ACAAAAGGGG TGGATTATAT ACATTCAAAA 1321 AAATTAATTC ATAGAGATCT TAAGCCAAGT AATATATTCT TAGTAGATAC AAAACAAGTA 1381 AAGATTGGAG ACTTTGGACT TGTAACATCT CTGAAAAATG ATGGAAAGCG AACAAGGAGT 1441 AAGGGAACTT TGCGATACAT GAGCCCAGAA CAGATTTCTT CGCAAGACTA TGGAAAGGAA 1501 GTGGACCTCT ACGCTTTGGG GCTAATTCTT GCTGAACTTC TTCATGTATG TGACACTGCT 1561 TTTGAAACAT CAAAGAAAAC TCTTCTACAG AAATTACTCT CAAAGAAACC TGAGGATCGA 1621 CCTAACACAT CTGAAATACT AAGGACCTTG ACTGTGTGGA AGAAAAGCCC AGAGAAAAAT 1681 GAACGACACA CATGTTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC 1741 TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC 1801 CCAGCCTGAA ATC