Construct: ORF ccsbBroadEn_14817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018950.1_s300c1, BRDN0000458246
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PSKH1 (5681)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5681 | PSKH1 | protein serine kinase H1 | NM_006742.3 | 99.6% | 38.7% | (many diffs) |
2 | mouse | 244631 | Pskh1 | protein serine kinase H1 | NM_173432.2 | 88.3% | 36.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 640
- ORF length:
- 543
- Sequence:
-
1 AGCGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG GCTGTGGGAC AAGCAAGGTC 121 CTTCCCGAGC CACCCAAGGA TGTCCAGCTG GATCTGGTCA AGAAGGTGGA GCCCTTCAGT 181 GGCACTAAGA GTGACGTGTA CAAGCACTTC ATCACAGAGG TGGACAGTGT TGGCCCTGTC 241 AAAGCCGGGT TCCCAGCAGC AAGTCAGTAT GCACACCCCT GCCCCGGTCC CCCGACTGCT 301 GGCCACACGG AGCCTCCCTC AGAACCACCA CGCAGGGCCA GGGTAGCTAA GTACAGGGCC 361 AAGTTTGACC CACGTGTTAC AGCTAAGTAT GACATCAAGG CCCTAATTGG CCGAGGCAGC 421 TTCAGCCGAG TGGTACGTGT AGAGCACCGG GCAACCCGGC AGCCGTATGC CATCAAGATG 481 ATTGAGACCA AGTACCGGGA GGGGCGGGAG GTGTGTGAGT CGGAGCTGCG TGTGCTGCGT 541 CGGGTGCGTC ATGCCAACAT CATCCAGCTG GTGGAGGTGT TCGAGACACA GGAGCGGGTG 601 TTACATGGTG ATGGAGCTGG CCACTGGTGG AGAGCTCTTT GACCGCATCA TTGCCAAGGG 661 CTCCTTCACC GAGCGTGACG CCACGCGGGT GCTGCAGATG GTGCTGGATG GCGTCCGGTA 721 TCTGCATGCA CTGGGCATCA CACACCGAGA CCTCAAACNT GAGAATCTGC TCTACTACCA 781 TCCCGGGCAC TGACTCCAAG ATCATCATCA CCGACTTCGG CCTGGCCAGT GCTCGCAAGA 841 AGGGTGGATG ACTGCNTGAT GAAGACCACC TGTGGCACGC CTGAGTACAT TGCCCCAGAA 901 GTCCTGGTCC GCAAGCCATA CACCAACTCA GTGGACATGT GGGCGCTGGG CGTCATTGCC 961 TACATCCTAC TCAGTGGCAC CATGCCGTTT GAGGATGACA ACCGTACCCG GCTGTACCGG 1021 CAGATCCTCA GGGGCAAGTA CAGTTACTCT GGGGAGCCCT GGCCTAGTGT GTCCAACCTG 1081 GCCAAGGACT TCATTGACCG CCTGCTGACA GTGGACCCTG GAGCCCGTAT GACTGCACTG 1141 CAGGCCCTGA GGCACCCGTG GGTGGTGAGC ATGGCTGCCT CTTCATCCAT GAAGAACCTG 1201 CACCGCTCCA TATCCCAGAA CCTCCTTAAA CGTGCCTCCT CGCGCTGCCA GAGCACCAAA 1261 TCTGCCCAGT CCACGCGTTC CAGCCGCTCC ACACGCTCCA ATAAGTCACG CCGTGTGCGG 1321 GAACGGGAGC TGCGGGAGCT CAACCTGCGC TACCAGCAGC AATACAATGG CTTGCCAACT 1381 TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG 1441 GTCACTATCA GTCAAAATAA AATCATTATT GCCATCCAGC TATCTAAG