Transcript: Human NM_006742.3

Homo sapiens protein serine kinase H1 (PSKH1), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
PSKH1 (5681)
Length:
3497
CDS:
189..1463

Additional Resources:

NCBI RefSeq record:
NM_006742.3
NBCI Gene record:
PSKH1 (5681)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147684 CATACTTAGCTGTAACACGT pXPR_003 GGG 278 22% 2 0.7977 PSKH1 PSKH1 76740
2 BRDN0001145277 AGGTGTTCGAGACACAGGAG pXPR_003 CGG 495 39% 2 0.6496 PSKH1 PSKH1 76741
3 BRDN0001149317 CACCGAGCGTGACGCCACGC pXPR_003 GGG 586 46% 2 -0.0688 PSKH1 PSKH1 76739
4 BRDN0001149057 CTGAGTTGGTGTATGGCTTG pXPR_003 CGG 815 64% 2 -0.4991 PSKH1 PSKH1 76738
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006742.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199804 GCACTAAGAGTGACGTGTACA pLKO.1 274 CDS 100% 4.950 6.930 N PSKH1 n/a
2 TRCN0000001711 CCGCTCCACACGCTCCAATAA pLKO.1 1373 CDS 100% 4.400 6.160 N PSKH1 n/a
3 TRCN0000277866 CCGCTCCACACGCTCCAATAA pLKO_005 1373 CDS 100% 4.400 6.160 N PSKH1 n/a
4 TRCN0000001712 GCACCGCTCCATATCCCAGAA pLKO.1 1289 CDS 100% 1.350 1.080 N PSKH1 n/a
5 TRCN0000277865 GCACCGCTCCATATCCCAGAA pLKO_005 1289 CDS 100% 1.350 1.080 N PSKH1 n/a
6 TRCN0000001709 GCTAAGTACAGGGCCAAGTTT pLKO.1 438 CDS 100% 5.625 3.938 N PSKH1 n/a
7 TRCN0000277867 GCTAAGTACAGGGCCAAGTTT pLKO_005 438 CDS 100% 5.625 3.938 N PSKH1 n/a
8 TRCN0000001708 TCCCTTCACAGCATTAACCTT pLKO.1 2777 3UTR 100% 3.000 2.100 N PSKH1 n/a
9 TRCN0000277922 TCCCTTCACAGCATTAACCTT pLKO_005 2777 3UTR 100% 3.000 2.100 N PSKH1 n/a
10 TRCN0000199111 CCGCCCTTGGTGCCTTCTTTG pLKO.1 2201 3UTR 100% 0.000 0.000 N PSKH1 n/a
11 TRCN0000001710 CAAGCCATACACCAACTCAGT pLKO.1 1001 CDS 100% 2.640 1.584 N PSKH1 n/a
12 TRCN0000297118 CAAGCCATACACCAACTCAGT pLKO_005 1001 CDS 100% 2.640 1.584 N PSKH1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006742.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489006 ACCAACCCCAATGGTCGTTTGGTC pLX_317 18.2% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_06794 pDONR223 100% 99.9% 99.7% None 17G>T n/a
3 ccsbBroad304_06794 pLX_304 0% 99.9% 99.7% V5 17G>T n/a
4 TRCN0000491257 TTGAAGTGCGGCCAAAAACCTACG pLX_317 12.1% 99.9% 99.7% V5 17G>T n/a
5 ccsbBroadEn_14817 pDONR223 100% 99.6% 38.7% None (many diffs) n/a
6 ccsbBroad304_14817 pLX_304 0% 99.6% 38.7% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000471655 TTTCTTGAACGCGGACCGTGTCTC pLX_317 30.2% 99.6% 38.7% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_15142 pDONR223 54.5% 33.6% 5.6% None 0_1ins319;1272_1273ins2185 n/a
9 ccsbBroad304_15142 pLX_304 0% 33.6% 5.6% V5 (not translated due to prior stop codon) 0_1ins319;1272_1273ins2185 n/a
10 TRCN0000474897 TCCGATTATTTCCCTTACGATCAA pLX_317 11.3% 33.6% 5.6% V5 (not translated due to prior stop codon) 0_1ins319;1272_1273ins2185 n/a
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