Transcript: Mouse NM_173432.2

Mus musculus protein serine kinase H1 (Pskh1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Pskh1 (244631)
Length:
3317
CDS:
138..1412

Additional Resources:

NCBI RefSeq record:
NM_173432.2
NBCI Gene record:
Pskh1 (244631)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_173432.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024460 GCGCTATCAACAGCAGTATAA pLKO.1 1385 CDS 100% 13.200 18.480 N Pskh1 n/a
2 TRCN0000412612 TGAGACACAGGAGCGTGTATA pLKO_005 623 CDS 100% 13.200 18.480 N Pskh1 n/a
3 TRCN0000360682 GAGCTCTTTGACCGGATTATT pLKO_005 672 CDS 100% 15.000 10.500 N Pskh1 n/a
4 TRCN0000432597 GCAAAGTACAGGGCCAAATTT pLKO_005 387 CDS 100% 15.000 10.500 N Pskh1 n/a
5 TRCN0000360613 TATGATATAAAGGCCCTAATT pLKO_005 429 CDS 100% 13.200 9.240 N Pskh1 n/a
6 TRCN0000024462 GTATGATATAAAGGCCCTAAT pLKO.1 428 CDS 100% 10.800 7.560 N Pskh1 n/a
7 TRCN0000024459 CCTACACCAATTCCGTGGATA pLKO.1 955 CDS 100% 4.950 3.465 N Pskh1 n/a
8 TRCN0000024463 GCCAAGGGTTCTTTCACTGAA pLKO.1 693 CDS 100% 4.950 3.465 N Pskh1 n/a
9 TRCN0000024461 CCATGAAGAATCTGCACCGAT pLKO.1 1225 CDS 100% 2.640 1.848 N Pskh1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173432.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489006 ACCAACCCCAATGGTCGTTTGGTC pLX_317 18.2% 88.5% 97.6% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_06794 pDONR223 100% 88.4% 97.4% None (many diffs) n/a
3 ccsbBroad304_06794 pLX_304 0% 88.4% 97.4% V5 (many diffs) n/a
4 TRCN0000491257 TTGAAGTGCGGCCAAAAACCTACG pLX_317 12.1% 88.4% 97.4% V5 (many diffs) n/a
5 ccsbBroadEn_14817 pDONR223 100% 88.3% 36.9% None (many diffs) n/a
6 ccsbBroad304_14817 pLX_304 0% 88.3% 36.9% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000471655 TTTCTTGAACGCGGACCGTGTCTC pLX_317 30.2% 88.3% 36.9% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_15142 pDONR223 54.5% 29.8% 3.9% None (many diffs) n/a
9 ccsbBroad304_15142 pLX_304 0% 29.8% 3.9% V5 (not translated due to prior stop codon) (many diffs) n/a
10 TRCN0000474897 TCCGATTATTTCCCTTACGATCAA pLX_317 11.3% 29.8% 3.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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