Construct: ORF ccsbBroadEn_14820

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF019057.1_s300c1, BRDN0000458675
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: PTK7 (5754)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	5754	PTK7	protein tyrosine kinase 7 (...	NM_002821.5	100%	100%
2	human	5754	PTK7	protein tyrosine kinase 7 (...	NM_001270398.1	96.9%	96.5%	(many diffs)
3	human	5754	PTK7	protein tyrosine kinase 7 (...	NM_152880.4	96.2%	96.2%	1497_1498ins120
4	human	5754	PTK7	protein tyrosine kinase 7 (...	NM_152882.4	94.7%	94.7%	1878_1879ins168
5	human	5754	PTK7	protein tyrosine kinase 7 (...	XM_011514765.2	93.2%	92.9%	(many diffs)
6	human	5754	PTK7	protein tyrosine kinase 7 (...	NM_152881.4	87.8%	87.7%	1228_1229ins390
7	human	5754	PTK7	protein tyrosine kinase 7 (...	XM_011514766.2	85%	84.5%	(many diffs)
8	human	5754	PTK7	protein tyrosine kinase 7 (...	NR_072997.2	71.3%		(many diffs)
9	human	5754	PTK7	protein tyrosine kinase 7 (...	NR_072998.2	70.5%		1_175del;2581_2582ins233;3153_3989del
10	human	5754	PTK7	protein tyrosine kinase 7 (...	XR_926286.2	70.3%		(many diffs)
11	mouse	71461	Ptk7	PTK7 protein tyrosine kinase 7	NM_175168.4	87.5%	91.8%	(many diffs)
12	mouse	71461	Ptk7	PTK7 protein tyrosine kinase 7	XM_006524922.2	86.6%	90.2%	(many diffs)
13	mouse	71461	Ptk7	PTK7 protein tyrosine kinase 7	XM_006524924.2	86.2%	90.7%	(many diffs)
14	mouse	71461	Ptk7	PTK7 protein tyrosine kinase 7	XM_006524923.1	85.7%	89.3%	(many diffs)
15	mouse	71461	Ptk7	PTK7 protein tyrosine kinase 7	XM_017317638.1	68.2%	72%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

3279

ORF length:

3210

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61	ttggcaccat gggagctgcg cggggatccc cggccagacc ccgccggttg cctctgctca
121	gcgtcctgct gctgccgctg ctgggcggta cccagacagc cattgtcttc atcaagcagc
181	cgtcctccca ggatgcactg caggggcgcc gggcgctgct tcgctgtgag gttgaggctc
241	cgggcccggt acatgtgtac tggctgctcg atggggcccc tgtccaggac acggagcggc
301	gtttcgccca gggcagcagc ctgagctttg cagctgtgga ccggctgcag gactctggca
361	ccttccagtg tgtggctcgg gatgatgtca ctggagaaga agcccgcagt gccaacgcct
421	ccttcaacat caaatggatt gaggcaggtc ctgtggtcct gaagcatcca gcctcggaag
481	ctgagatcca gccacagacc caggtcacac ttcgttgcca cattgatggg caccctcggc
541	ccacctacca atggttccga gatgggaccc ccctttctga tggtcagagc aaccacacag
601	tcagcagcaa ggagcggaac ctgacgctcc ggccagctgg tcctgagcat agtgggctgt
661	attcctgctg cgcccacagt gcttttggcc aggcttgcag cagccagaac ttcaccttga
721	gcattgctga tgaaagcttt gccagggtgg tgctggcacc ccaggacgtg gtagtagcga
781	ggtatgagga ggccatgttc cattgccagt tctcagccca gccacccccg agcctgcagt
841	ggctctttga ggatgagact cccatcacta accgcagtcg ccccccacac ctccgcagag
901	ccacagtgtt tgccaacggg tctctgctgc tgacccaggt ccggccacgc aatgcaggga
961	tctaccgctg cattggccag gggcagaggg gcccacccat catcctggaa gccacacttc
1021	acctagcaga gattgaagac atgccgctat ttgagccacg ggtgtttaca gctggcagcg
1081	aggagcgtgt gacctgcctt ccccccaagg gtctgccaga gcccagcgtg tggtgggagc
1141	acgcgggagt ccggctgccc acccatggca gggtctacca gaagggccac gagctggtgt
1201	tggccaatat tgctgaaagt gatgctggtg tctacacctg ccacgcggcc aacctggctg
1261	gtcagcggag acaggatgtc aacatcactg tggccactgt gccctcctgg ctgaagaagc
1321	cccaagacag ccagctggag gagggcaaac ccggctactt ggattgcctg acccaggcca
1381	caccaaaacc tacagttgtc tggtacagaa accagatgct catctcagag gactcacggt
1441	tcgaggtctt caagaatggg accttgcgca tcaacagcgt ggaggtgtat gatgggacat
1501	ggtaccgttg tatgagcagc accccagccg gcagcatcga ggcgcaagcc cgtgtccaag
1561	tgctggaaaa gctcaagttc acaccaccac cccagccaca gcagtgcatg gagtttgaca
1621	aggaggccac ggtgccctgt tcagccacag gccgagagaa gcccactatt aagtgggaac
1681	gggcagatgg gagcagcctc ccagagtggg tgacagacaa cgctgggacc ctgcattttg
1741	cccgggtgac tcgagatgac gctggcaact acacttgcat tgcctccaac gggccgcagg
1801	gccagattcg tgcccatgtc cagctcactg tggcagtttt tatcaccttc aaagtggaac
1861	cagagcgtac gactgtgtac cagggccaca cagccctact gcagtgcgag gcccaggggg
1921	accccaagcc gctgattcag tggaaaggca aggaccgcat cctggacccc accaagctgg
1981	gacccaggat gcacatcttc cagaatggct ccctggtgat ccatgacgtg gcccctgagg
2041	actcaggccg ctacacctgc attgcaggca acagctgcaa catcaagcac acggaggccc
2101	ccctctatgt cgtggacaag cctgtgccgg aggagtcgga gggccctggc agccctcccc
2161	cctacaagat gatccagacc attgggttgt cggtgggtgc cgctgtggcc tacatcattg
2221	ccgtgctggg cctcatgttc tactgcaaga agcgctgcaa agccaagcgg ctgcagaagc
2281	agcccgaggg cgaggagcca gagatggaat gcctcaacgg tgggcctttg cagaacgggc
2341	agccctcagc agagatccaa gaagaagtgg ccttgaccag cttgggctcc ggccccgcgg
2401	ccaccaacaa acgccacagc acaagtgata agatgcactt cccacggtct agcctgcagc
2461	ccatcaccac gctggggaag agtgagtttg gggaggtgtt cctggcaaag gctcagggct
2521	tggaggaggg agtggcagag accctggtac ttgtgaagag cctgcagagc aaggatgagc
2581	agcagcagct ggacttccgg agggagttgg agatgtttgg gaagctgaac cacgccaacg
2641	tggtgcggct cctggggctg tgccgggagg ctgagcccca ctacatggtg ctggaatatg
2701	tggatctggg agacctcaag cagttcctga ggatttccaa gagcaaggat gaaaaattga
2761	agtcacagcc cctcagcacc aagcagaagg tggccctatg cacccaggta gccctgggca
2821	tggagcacct gtccaacaac cgctttgtgc ataaggactt ggctgcgcgt aactgcctgg
2881	tcagtgccca gagacaagtg aaggtgtctg ccctgggcct cagcaaggat gtgtacaaca
2941	gtgagtacta ccacttccgc caggcctggg tgccgctgcg ctggatgtcc cccgaggcca
3001	tcctggaggg tgacttctct accaagtctg atgtctgggc cttcggtgtg ctgatgtggg
3061	aagtgtttac acatggagag atgccccatg gtgggcaggc agatgatgaa gtactggcag
3121	atttgcaggc tgggaaggct agacttcctc agcccgaggg ctgcccttcc aaactctatc
3181	ggctgatgca gcgctgctgg gccctcagcc ccaaggaccg gccctccttc agtgagattg
3241	ccagcgccct gggagacagc accgtggaca gcaagccgtt gccaactttc ttgtacaaag
3301	ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

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