Transcript: Human NM_152881.4

Homo sapiens protein tyrosine kinase 7 (inactive) (PTK7), transcript variant PTK7-3, mRNA.

Source:
NCBI, updated 2019-07-23
Taxon:
Homo sapiens (human)
Gene:
PTK7 (5754)
Length:
3832
CDS:
176..2998

Additional Resources:

NCBI RefSeq record:
NM_152881.4
NBCI Gene record:
PTK7 (5754)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001487092 GAGCGTACGACTGTGTACCA pXPR_003 GGG 1421 50% 9 1.217 PTK7 PTK7 78022
2 BRDN0001147716 GGTAGTAGCGAGGTATGAGG pXPR_003 AGG 718 25% 5 0.6767 PTK7 PTK7 78021
3 BRDN0001146728 GCAGCCAGTACACATGTACC pXPR_003 GGG 182 6% 2 0.529 PTK7 PTK7 78024
4 BRDN0001147225 GCTCTGACCATCAGAAAGGG pXPR_003 GGG 505 18% 4 0.1679 PTK7 PTK7 78023
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152881.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195464 CCACTACATGGTGCTGGAATA pLKO.1 2395 CDS 100% 10.800 15.120 N PTK7 n/a
2 TRCN0000199181 CGGTACCCAGACAGCCATTGT pLKO.1 253 CDS 100% 0.000 0.000 N PTK7 n/a
3 TRCN0000197147 GATGAAGTACTGGCAGATTTG pLKO.1 2822 CDS 100% 10.800 7.560 N PTK7 n/a
4 TRCN0000199565 GCCACTCATCTGCCAACTTTG pLKO.1 3327 3UTR 100% 10.800 7.560 N PTK7 n/a
5 TRCN0000006433 CCACAGCACAAGTGATAAGAT pLKO.1 2131 CDS 100% 5.625 3.938 N PTK7 n/a
6 TRCN0000006432 CCTTGAGCATTGCTGATGAAA pLKO.1 822 CDS 100% 5.625 3.938 N PTK7 n/a
7 TRCN0000006431 CACAGGGTTAATGAGTCTCTT pLKO.1 3418 3UTR 100% 4.950 3.465 N PTK7 n/a
8 TRCN0000195324 CCATGTTCCATTGCCAGTTCT pLKO.1 900 CDS 100% 4.950 3.465 N PTK7 n/a
9 TRCN0000006434 CCTCATGTTCTACTGCAAGAA pLKO.1 1948 CDS 100% 4.950 3.465 N PTK7 n/a
10 TRCN0000006435 CCTGAGGATTTCCAAGAGCAA pLKO.1 2443 CDS 100% 2.640 1.848 N PTK7 n/a
11 TRCN0000023609 GCCATGTTCCATTGCCAGTTT pLKO.1 899 CDS 100% 4.950 3.465 N Ptk7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152881.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14820 pDONR223 0% 87.8% 87.7% None 1228_1229ins390 n/a
2 ccsbBroad304_14820 pLX_304 0% 87.8% 87.7% V5 1228_1229ins390 n/a
3 TRCN0000487743 TTATCTCACTGTGTCGAGGCGGCT pLX_317 7.7% 87.8% 87.7% V5 (not translated due to prior stop codon) 1228_1229ins390 n/a
4 TRCN0000488811 CATGTACGTATCCATCCGGAACCC pLX_317 7.6% 87.8% 87.6% V5 1228_1229ins390;2820_2821insG n/a
5 TRCN0000487971 CCAGCCTACCTCCGCCCTACGATC pLX_317 7.7% 87.8% 87.7% V5 (not translated due to prior stop codon) 1228_1229ins390;1461G>A n/a
6 TRCN0000488980 CTCGTATACCCCACCTCATTGTGC pLX_317 7.6% 87.7% 87.6% V5 1228_1229ins390;1461G>A;2820_2821insG n/a
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