Construct: ORF ccsbBroadEn_14823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011884.2_s300c1, BRDN0000458376
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K2 (5871)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_001307990.2 | 99.9% | 100% | 1794C>T |
| 2 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_004579.5 | 98.9% | 99% | 1274_1297del;1818C>T |
| 3 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018093.2 | 94.5% | 94.5% | 529_530ins132;1662C>T |
| 4 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448629.1 | 94.3% | 94.4% | 1167_1286del;1394_1417del;1938C>T |
| 5 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_011545204.3 | 93.6% | 93.6% | 529_530ins132;1142_1165del;1686C>T |
| 6 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448630.1 | 91.7% | 91.6% | (many diffs) |
| 7 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448631.1 | 89.2% | 89.3% | (many diffs) |
| 8 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018095.2 | 74.5% | 74.5% | (many diffs) |
| 9 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448633.1 | 73.7% | 73.8% | (many diffs) |
| 10 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448634.1 | 66.9% | 66.9% | 1167_1286del;1394_1417del;1872_1872delAins709 |
| 11 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XR_002957155.1 | 45.8% | (many diffs) | |
| 12 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_009006.3 | 87.1% | 93.4% | (many diffs) |
| 13 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531755.2 | 77.5% | 82.3% | (many diffs) |
| 14 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531756.2 | 77.5% | 82.3% | (many diffs) |
| 15 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531757.2 | 77.5% | 82.3% | (many diffs) |
| 16 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318211.1 | 77.4% | 83.1% | (many diffs) |
| 17 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318212.1 | 77.4% | 83.1% | (many diffs) |
| 18 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_001291787.1 | 77% | 82.5% | (many diffs) |
| 19 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531760.3 | 77% | 82.5% | (many diffs) |
| 20 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531758.3 | 76.3% | 82.2% | (many diffs) |
| 21 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XR_001782591.1 | 45.7% | (many diffs) | |
| 22 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NR_117093.1 | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2502
- ORF length:
- 2436
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggc gctgctgcgg gatgtgtcgc tgcaggaccc gcgggaccgc ttcgagctgc 121 tgcagcgcgt gggggccggg acctatggcg acgtctacaa ggcccgcgac acggtcacgt 181 ccgaactggc cgccgtgaag atagtcaagc tagacccagg ggacgacatc agctccctcc 241 agcaggaaat caccatcctg cgtgagtgcc gccaccccaa tgtggtggcc tacattggca 301 gctacctcag gaatgaccgc ttgtggatct gcatggagtt ctgcggaggg ggctccctgc 361 aggagattta ccatgccact gggcccctgg aggagcggca gattgcctac gtctgccgag 421 aggcactgaa ggggctccac cacctgcatt ctcaggggaa gatccacaga gacatcaagg 481 gagccaacct tctcctcact ctccagggag atgtcaaact ggctgacttt ggggtgtcag 541 gcgagctgac agcgtctgtg gccaagagga ggtctttcat tgggactccc tactggatgg 601 ctcccgaggt ggctgctgtg gagcgcaaag gtggctacaa tgagctatgt gacgtctggg 661 ccctgggcat cactgccatt gagctgggcg agctgcagcc ccctctgttc cacctgcacc 721 ccatgagggc cctgatgctc atgtcgaaga gcagcttcca gccgcccaaa ctgagagata 781 agactcgctg gacccagaat ttccaccact ttctcaaact ggccctgacc aagaatccta 841 agaagaggcc gacagcagag aagctcctgc agcacccgtt cacgactcag cagctccctc 901 gggccctcct cacacagctg ctggacaaag ccagtgaccc tcatctgggg accccctccc 961 ctgaggactg tgagctggag acctatgaca tgtttccaga caccattcac tcccgggggc 1021 agcacggccc agccgagagg accccctcgg agatccagtt tcaccaggtg aaatttggcg 1081 ccccacgcag gaaggaaact gacccactga atgagccgtg ggaggaagag tggacactac 1141 tgggaaagga agagttgagt gggagcctgc tgcagtcggt ccaggaggcc ctggaggaaa 1201 ggagtctgac tattcggtca gcctcagaat tccaggagct ggactcccca gacgatacca 1261 tgggaaccat caagcgggcc ccgttcctag ggccactccc cactgaccct ccagcagagg 1321 agcctctgtc cagtccccca ggccccaaca gctccccact gctgcccacg gcctgggcca 1381 ccatgaagca gcgggaggat cctgagaggt catcctgcca cgggctcccc ccaactccca 1441 aggtgcatat gggcgcctgc ttctccaagg tcttcaatgg ctgccccctg cggatccacg 1501 ctgctgtcac ctggattcac cctgttactc gggaccagtt cctggtggta ggggccgagg 1561 aaggcatcta cacactcaac ctgcatgaac tgcatgagga tacgctggag aagctgattt 1621 cacatcgctg ctcctggctc tactgcgtga acaacgtgct gctgtcactc tcagggaaat 1681 ccacgcacat ctgggcccat gacctcccag gcctgtttga gcagcggagg ctacagcaac 1741 aggttcccct ctccatcccc accaaccgcc tcacccagcg catcatcccc aggcgctttg 1801 ctctgtccac caagattcct gacaccaaag gctgcttgca gtgtcgtgtg gtgcggaatc 1861 cctacacggg tgccaccttc ctgctggccg ccctgcccac cagcctgctc ctgctgcagt 1921 ggtatgagcc gctgcagaag tttctgctgc tgaagaactt ctccagccct ctgcccagcc 1981 cagctgggat gctggagccg ctggtgctgg atgggaagga gctgccgcag gtgtgtgttg 2041 gggccgaggg gcctgagggg cccggctgcc gcgtcctgtt ccatgtcctg cccctggagg 2101 ctggcctgac gcccgacatc ctcatcccac ctgaggggat cccaggctcg gcccagcagg 2161 tgatccaggt ggacagggac acaatcctag tcagctttga acgctgtgtg aggattgtca 2221 acatgcaggg cgagcccacg gccacactgg cacctgagct gacctttgat ttccccatcg 2281 agactgtggt gtgcctgcag gacagtgtgc tggccttctg gagccatggg atgcaaggcc 2341 gaagcctgga taccaatgag gtgacccagg agatcacaga tgaaacaagg atcttccgag 2401 tgcttggggc ccacagagac atcatcctgg agagcattcc cactgacaac ccagaggcgc 2461 acagcaacct ctacatcctc acgggccacc agagcaccta ctacccaact ttcttgtaca 2521 aagttggcat tataagaaag cattgcttat caatttgttg caacgaac