Construct: ORF ccsbBroadEn_14885

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017677.1_s300c1, BRDN0000385030
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NPHS2 (7827)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7827 NPHS2 NPHS2 stomatin family membe... NM_001297575.2 99.6% 100% 102A>G;288C>T;834A>G
2 human 7827 NPHS2 NPHS2 stomatin family membe... XM_017002298.1 86% 80% (many diffs)
3 human 7827 NPHS2 NPHS2 stomatin family membe... NM_014625.4 81.9% 82.2% (many diffs)
4 human 7827 NPHS2 NPHS2 stomatin family membe... XM_005245483.3 78.5% 66.8% (many diffs)
5 human 7827 NPHS2 NPHS2 stomatin family membe... XM_017002299.1 56.2% 56.5% 102A>G;288C>T;534_535ins411
6 mouse 170484 Nphs2 nephrosis 2, podocin NM_130456.4 69.8% 69.3% (many diffs)
7 mouse 170484 Nphs2 nephrosis 2, podocin XM_006496684.3 49.4% 40.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1011
ORF length:
945
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA GAGGAGGGCG CGGAGCTCCT CCAGGGAGTC CCGCGGGCGA GGCGGCAGGA
121CTCCGCACAA GGAGAACAAG AGGGCAAAGG CCGAGAGGAG CGGCGGGGGC CGCGGGCGCC
181AGGAggctgg gcccgagccg tcgggctccg gacgggcggg gaccccgggg gagccccgag
241cgcccgccgc cacggtggtg gacGTGGATG AGGTCCGAGG CTCCGGCGAG GAGGGCACCG
301AGGTGGTGGC GCTGTTGGAG AGCGAGCGGC CCGAGGAAGG TACCAAATCC TCTGGCTTAG
361GGGCCTGTGA GTGGCTTCTT GTCCTCATTT CCCTGCTCTT CATCATCATG ACCTTCCCTT
421TTTCCATCTG GTTCTGCGTA AAGGTTGTAC AAGAGTATGA AAGAGTAATT ATATTCCGAC
481TGGGACATCT GCTTCCTGGA AGAGCCAAAG GCCCTGGTCT TTTCTTTTTT TTGCCCTGCC
541TGGATACCTA CCACAAGGTT GACCTTCGTC TCCAAACTCT GGAGATACCT TTTCATGAGG
601TTGCCTTGGA TTCAGTGACC TGTATTTGGG GAATCAAAGT GGAGAGAATA GAAATTAAAG
661ATGTGAGGTT GCCAGCTGGG CTTCAGCACT CACTGGCTGT GGAGGCTGAA GCGCAAAGAC
721AAGCCAAAGT GCGGATGATT GCTGCAGAAG CGGAAAAGGC TGCTTCTGAG TCCCTGAGGA
781TGGCAGCTGA GATTCTGTCA GGCACCCCTG CTGCCGTTCA GCTTCGATAC CTCCACACCC
841TTCAGTCTCT GTCCACAGAG AAGCCTTCCA CTGTGGTTTT ACCTTTGCCA TTTGACCTGC
901TGAATTGCCT GTCTTCTCCC AGCAACAGAA CTCAGGGAAG CCTCCCCTTC CCAAGTCCTT
961CCAAACCTGT TGAGCCACTA AATCCTAAAA AGAAAGACTC TCCCATGTTA TACCCAACTT
1021TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)

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