Construct: ORF ccsbBroadEn_14901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018980.1_s300c1, BRDN0000458276
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CAMK1 (8536)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | NM_003656.5 | 99.4% | 62.9% | (many diffs) |
| 2 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_005265516.2 | 89.1% | 56.4% | (many diffs) |
| 3 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_017007354.1 | 87.5% | 51% | (many diffs) |
| 4 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_024453796.1 | 82.7% | 46.2% | (many diffs) |
| 5 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_005265517.3 | 78.4% | 45.7% | (many diffs) |
| 6 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XR_940505.2 | 68.6% | (many diffs) | |
| 7 | mouse | 52163 | Camk1 | calcium/calmodulin-dependen... | NM_133926.2 | 89.3% | 61.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 129
- ORF end:
- 840
- ORF length:
- 711
- Sequence:
-
1 GGGGGGTGGG GGCCGTGAAA AGCCGGGCCC AATATGATTT TATTTTGACT GATAGTGACC 61 TGTTCGTTGC ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG 121 TTGGCACCAT GCTGGGGGCA GTGGAAGGCC CCAGGTGGAA GCAGGCGGAG GACATTAGAG 181 ACATCTACGA CTTCCGAGAT GTTCTGGGCA CGGGGGCCTT CTCGGAGGTG ATCCTGGCAG 241 AAGATAAGAG GACGCAGAAG CTGGTGGCCA TCAAATGCAT TGCCAAGGAG GCCCTGGAGG 301 GCAAGGAAGG CAGCATGGAG AATGAGATTG CTGTCCTGCA CAAGATCAAG CACCCCAACA 361 TTGTAGCCCT GGATGACATC TATGAGAGTG GGGGCCACCT CTACCTCATC ATGCAGCTGG 421 TGTCGGGTGG GGAGCTCTTT GACCGTATTG TGGAAAAAGG CTTCTACACG GAGCGGGACG 481 CCAGCCGCCT CATCTTCCAG GTGCTGGATG CTGTGAAATA CCTGCNTGAC NTGGGCATTG 541 TACACCGGGA TCTCAAGCCA GAGAATCTGC TGTACTACAG CCNGGATGAA GACTCCAAAA 601 TCATGATCTC CGACTTTGGC CTCTCCAAGA TGGAGGACCC GGGCAGTGTG CTCTCCACCG 661 CCTGTGGAAC TCCGGGATAC GTGGCCCCTG AAGTCCTGGC CCAGAAGCCC TACAGCAAGG 721 CTGTGGATTG CTGGTCCATA GGTGTCATCG CCTACATCTT GCTCTGCGGT TACCCTCCCT 781 TCTATGACGA GAATGATGCC AAACTCTTTN NACAGATTTT GAAGGCCGAG TACGAGTTTT 841 GACTCTCCTT ACTGGGACGA CATCTCTGAC TCTGCCAAAG ATTTCATCCG GCACTTGATG 901 GAGAAGGACC CAGAGAAAAG ATTCACCTGT GAGCAGGCCT TGCAGCACCC ATGGATTGCA 961 GGAGATACAG CTCTAGATAA GAATATCCAC CAGTCGGTGA GTGAGCAGAT CAAGAAGAAC 1021 TTTGCCAAGA GCAAGTGGAA GCAAGCCTTC AATGCCACGG CTGTGGTGCG GCACATGAGG 1081 AAACTGCAGC TGGGCACCAG CCAGGAGGGG CAGGGGCAGA CGGCGAGCCA TGGGGAGCTG 1141 CTGACACCAG TGGCTGGGGG GCCGGCAGCT GGCTGTTGCT GTCGAGACTG CTGCGTGGAG 1201 CCGGGCACAG AACTGTCCCC CACACTGCCC CACCAGCTCT TGCCAACTTT CTTGTACAAA 1261 GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT 1321 CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG 1381 GTCATAGCTG TTTCCC