Construct: ORF ccsbBroadEn_14906

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018906.1_s300c1, BRDN0000458202
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CASK (8573)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8573 CASK calcium/calmodulin dependen... NM_001126054.2 98.5% 26.1% (many diffs)
2 human 8573 CASK calcium/calmodulin dependen... NM_001126055.2 98.4% 26.1% (many diffs)
3 human 8573 CASK calcium/calmodulin dependen... XM_011543995.2 97.7% 25.9% (many diffs)
4 human 8573 CASK calcium/calmodulin dependen... XM_006724566.3 97% 26.5% (many diffs)
5 human 8573 CASK calcium/calmodulin dependen... NM_001367721.1 96.6% 25.3% (many diffs)
6 human 8573 CASK calcium/calmodulin dependen... XM_011543996.2 96.4% 26.2% (many diffs)
7 human 8573 CASK calcium/calmodulin dependen... XM_011543994.2 96.1% 25.6% (many diffs)
8 human 8573 CASK calcium/calmodulin dependen... NM_003688.3 96.1% 25.5% (many diffs)
9 human 8573 CASK calcium/calmodulin dependen... XM_005272686.4 95.9% 25.5% (many diffs)
10 human 8573 CASK calcium/calmodulin dependen... XM_011543993.2 95.3% 25.2% (many diffs)
11 human 8573 CASK calcium/calmodulin dependen... XM_024452473.1 76.6% 5.6% (many diffs)
12 human 8573 CASK calcium/calmodulin dependen... XM_011543997.3 76% 5.4% (many diffs)
13 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527561.3 93.7% 26.1% (many diffs)
14 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318369.1 93.6% 25.7% (many diffs)
15 mouse 12361 Cask calcium/calmodulin-dependen... NM_009806.3 93.5% 26.1% (many diffs)
16 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318370.1 93.1% 25.8% (many diffs)
17 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247441.2 92.9% 25.9% (many diffs)
18 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318371.1 92.3% 26% (many diffs)
19 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247440.2 92.2% 25.8% (many diffs)
20 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247439.2 92.1% 25.4% (many diffs)
21 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284503.1 91.9% 25.3% (many diffs)
22 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284504.1 91.7% 26.7% (many diffs)
23 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318368.1 91.6% 25.5% (many diffs)
24 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527560.3 91.4% 25.5% (many diffs)
25 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247438.2 91.3% 25.1% (many diffs)
26 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284505.1 91.2% 25.5% (many diffs)
27 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318367.1 90.8% 25.2% (many diffs)
28 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247436.2 89.6% 24.6% (many diffs)
29 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318366.1 89% 24.8% (many diffs)
30 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247437.1 88.4% 24.9% (many diffs)
31 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247435.2 88.2% 24.3% (many diffs)
32 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247433.1 88.1% 24.3% (many diffs)
33 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247431.1 87.5% 24% (many diffs)
34 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247432.1 87.1% 24.1% (many diffs)
35 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247434.1 86.9% 24.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
98
ORF end:
806
ORF length:
708
Sequence:
1AACGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT
61TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GCCGACGACG ACGTGCTGTT
121CGAGGATGTG TACGAGCTGT GCGAGGTGAT CGGAAAGGGT CCCTTCAGTG TTGTACGACG
181ATGTATCAAC AGAGAAACTG GGCAACAATT TGCTGTAAAA ATTGTTGATG TAGCCAAGTT
241CACATCAAGT CCAGGGTTAA GTACAGAAGA TCTAAAGCGG GAAGCCAGTA TCTGTCATAT
301GCTGAAACAT CCACACATTG TAGAGTTATT GGAGACATAT AGCTCAGATG GAATGCTTTA
361CATGGTTTTC GAATTTATGG ATGGAGCAGA TCTGTGTTTT GAAATCGTAA AGCGAGCTGA
421CGCTGGTTTT GTGTACAGTG AAGCTGTAGC CAGCCATTAT ATGAGACAGA TACTGGAAGC
481TCTACGCTAC TGCCATGATA ATAACATAAT TCACAGGGAT GTGAAGCCCC ACTGTGTTCT
541CCTTGCCTCA AAAGAAAACT CGGCACCTGT TAAACTTGGA GGCTTTGGGG TAGCTATTCA
601ATTAGGGGAG TCTGGACTTG TAGCTGGAGG ACGTGTTGGA ACACCTCATT TTATGGCACC
661AGAAGTGGTC AAAAGAGAGC CTTACGGAAA GCCTGTAGAC GTCTGGGGGT GCGGTGTGAT
721CCTTTTTATC CTGCTCAGTG GTTGTTTGCC TTTTTACGGA ACCAAGGAAA GATTGTTTGA
781AGGCATTATT AAAGGAAAAT ATAGATGAAT CCAAGGCAGT GGAGCCATAT CTCTGAAAGT
841GCCAAGACCT AGTACGTCGC ATGCTGATGC TGGATCCAGC TGAAAGGATC CACTGTTTTA
901TGAAGCCACT GAATCACCCA TGgcttaagg agcgggatcg ttacgcctac aagattcatc
961ttccagaaac agtagagcag ctgaggaaat tcaatgcaag gaggaaacta aagggtgcag
1021tactagccgc tgtgtcaagt cacaaattca actcattcta tggggatccc cctgaagagt
1081taccagattt ctccgaagac cctacctcct caggacttct agcagcagaa agagcagtct
1141cacaggtgct ggacagcctg gaagagattc atgcgcttac agactgcagt gaaaaggacc
1201tagattttct acacagtgtt ttccaggatc agcatcttca cacactacta gatctgtatg
1261acaaaattaa cacaaagtct tcaccacaaa tcaggaatcc tccaagcgat gcagtacaga
1321gagccaaaga ggtattggaa gaaatttcat gttaccctga gaataacgac gcaaaggaac
1381taaagcgtat tttaacacaa cctcatttca tggccttact tcagactcac gacgtagtgg
1441cacatgaagt ttacagtgat gaagcattga gggtcacacc tcctcccacc tctccctatt
1501taaacggcga ttctccagaa agtgctaacg gagacatgga tatggagaat gtgaccagag
1561ttcggctggt acagtttcaa aagaacacag atgaaccaat gggaatcact ttaaaaatga
1621atgaactaaa tcattgtatt gttgcaagaa ttatgcatgg gggcatgatt cacaggcaag
1681gtacacttca tgttggtgat gaaattcgag aaatcaatgg catcagtgtg gctaaccaaa
1741cagtggaaca actgcaaaaa atgcttaggg aaatgcgggg gagtattacc ttcaagattg
1801tgccaagtta ccgcactcag tcttcgtcct gtgaggactt gccatcaact acccaaccaa
1861aaggacgaca gatctatgta agagcacaat ttgaatatga tccagccaag gatgacctca
1921tcccctgtaa agaagctggc attcgattca gagttggtga catcatccag attattagta
1981aggatgatca taattggtgg caggGTAACC TGAAAAACTC CCAAAAATTG GACTGCAGTC
2041TCATTCCTTC TTCCTGACTT TCAGGAATTG CGAGTAGCTT GCATGNCCAT GGAGAGACCC
2101AAACAGGAGC AGCAGCCCAG CTGTACTTGT TTGCAAGAAA AAAGAAGCAG TACAAAGATA
2161AATATTTGGC AAAGCACAAT GCAGTGTTNG ATCAATTAGA TCTTGTCACA TATGAAGAAG
2221TAGTAAAACT GCCAGCATTC AAGAGGAAAA CACTAGTCTT ATTAGGCGCA CATGGTGTTG
2281GGAGAAGACA CATAAAAAAC ACTCTCATCA CAAAGCACCC AGACCGGTTT GCGTACCCTA
2341TTCCACATAC AACCAGACCT CCAAAGAAAG ACGAAGAAAA TGGAAAGAAT TATTACTTTG
2401TATCTCATGA CCAAATGATG CAAGACATCT CTAATAACGA GTACTTGGAG TACGGCAGCC
2461ACGAGGATGC GATGTATGGG ACAAAACTGG AGACCATCCG GAAGATCCAC GAGCAGGGGC
2521TGATTGCAAT ACTGGACGTG GAGCCTCAGG CACTGAAGGT CCTGAGAACT GCAGAGTTTG
2581CTCCTTTTGT TGTTTTCATT GCTGCACCAA CTATTACTCC AGGTTTAAAT GAGGATGAAT
2641CTCTTCAGCG TCTGCAGAAG GAGTCTGACA TCTTACAGAG AACATATGCA CACTACTTCG
2701ATCTCACAAT TATCAACAAT GAAATTGATG AGACAATCAG ACATCTGGAG GAAGCTGTTG
2761AGCTCGTGTG CACAGCCCCA CAGTGGGTCC CTGTCTCCTG GGTCTATTTG CCAACTTTCT
2821TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA
2881CTATCAGTCA AAATAAAATC ATTATTGCCA TCCCAGCTTG ATTT

Download FASTA (ORF) (Full)