Construct: ORF ccsbBroadEn_14933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018959.1_s300c1, BRDN0000458255
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAGI1 (9223)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001033057.2 | 99.8% | 15.3% | (many diffs) |
| 2 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_004742.3 | 81.7% | 17.9% | (many diffs) |
| 3 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365905.1 | 81.6% | 17.8% | (many diffs) |
| 4 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_015520.2 | 80.9% | 17.4% | (many diffs) |
| 5 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365904.2 | 80.8% | 17.4% | (many diffs) |
| 6 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365903.2 | 80.7% | 17.4% | (many diffs) |
| 7 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505600.1 | 89.2% | 15.1% | (many diffs) |
| 8 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505588.1 | 89.1% | 15.1% | (many diffs) |
| 9 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505587.1 | 89.1% | 15.1% | (many diffs) |
| 10 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505581.1 | 87.4% | 14.8% | (many diffs) |
| 11 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505582.1 | 87.4% | 14.8% | (many diffs) |
| 12 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505584.1 | 87.4% | 14.8% | (many diffs) |
| 13 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505580.1 | 87.4% | 14.8% | (many diffs) |
| 14 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505583.1 | 87.3% | 14.8% | (many diffs) |
| 15 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001029850.4 | 86.8% | 14.9% | (many diffs) |
| 16 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286784.1 | 86.7% | 14.9% | (many diffs) |
| 17 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505585.1 | 86.7% | 14.9% | (many diffs) |
| 18 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505586.1 | 86.7% | 14.9% | (many diffs) |
| 19 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505590.1 | 84.7% | 15.8% | (many diffs) |
| 20 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505589.1 | 83.1% | 15.5% | (many diffs) |
| 21 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_011241222.2 | 74.9% | 18.1% | (many diffs) |
| 22 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505591.1 | 74.5% | 16.6% | (many diffs) |
| 23 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286786.1 | 73.5% | 17.8% | (many diffs) |
| 24 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505601.2 | 73.4% | 17.2% | (many diffs) |
| 25 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286785.1 | 73.4% | 17.1% | (many diffs) |
| 26 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505593.2 | 73.4% | 17.1% | (many diffs) |
| 27 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505592.1 | 73.3% | 17.1% | (many diffs) |
| 28 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505598.3 | 73.2% | 3.9% | (many diffs) |
| 29 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505599.3 | 73.2% | 3.9% | (many diffs) |
| 30 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001083320.2 | 73.2% | 4.4% | (many diffs) |
| 31 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505594.3 | 73.2% | 3.9% | (many diffs) |
| 32 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505595.3 | 73.2% | 3.9% | (many diffs) |
| 33 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505596.2 | 73.2% | 3.9% | (many diffs) |
| 34 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505597.3 | 73.2% | 3.9% | (many diffs) |
| 35 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_011241223.2 | 73.2% | 3.9% | (many diffs) |
| 36 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_017321399.1 | 72.6% | 17.3% | (many diffs) |
| 37 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_010367.3 | 69.7% | 18.7% | (many diffs) |
| 38 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_017321400.1 | 69.1% | 18.1% | (many diffs) |
| 39 | mouse | 14924 | Magi1 | membrane associated guanyla... | NR_104594.1 | 63.7% | (many diffs) | |
| 40 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286788.1 | 60.1% | 5.5% | (many diffs) |
| 41 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001083321.2 | 60% | 4.8% | (many diffs) |
| 42 | mouse | 14924 | Magi1 | membrane associated guanyla... | XR_377424.2 | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 795
- ORF length:
- 696
- Sequence:
-
1 TAGGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GTCCAAAGTG ATCCAGAAGA 121 AGAACCACTG GACTAGCAGG GTTCACGAAT GCACCGTGAA GCGGGGACCC CAGGGCGAGC 181 TGGGGGTGAC GGTGCTGGGA GGCGCGGAGC ACGGGGAGTT TCCGTACGTC GGAGCGGTGG 241 CGGCGGTCGA GGCAGCGGGG CTTCCCGGCG GCGGCGAGGG CCCGAGGCTG GGCGAAGGGG 301 AGCTGCTTCT GGAGGTGCAG GGGGTCCGGG TGTCCGGCTT GCCCCGCTAT GACGTGCTGG 361 GGGTCATCGA CAGCTGCAAG GAGGCCGTCA CCTTCAAGGC CGTCAGACAA GGAGGAAGGC 421 TGAACAAGGA CCTGCGACAT TTTCTCAATC AGCGATTCCA GAAGGGGTCT CCTGATCATG 481 AGCTCCAGCA GACCATAAGG GATAACCTTT ACCGCCATGC TGTGCCTTGC ACAACCCGAT 541 CTCCCAGAGA AGGAGAAGTG CCTGGCGTGG ACTATAACTT TCTGACTGTG AAGGAGTTCT 601 TGGACCTCGA GCAGAGTGGG ACTCTTCTGG AAGTCGGCAC CTATGAAGGA AACTATTATG 661 GGACACCCAA GCCTCCTAGC CAGCCAGTCA GTGGGAAAGT GATCACGACG GATGCCTTGC 721 ACAGCCTTCA GTCTGGCTCT AAGCAGTCGA CCCCGAAGCG AACCAAGTCC TACATGATAT 781 GCAAAATGCT GGCATAGTCC ACGCGGAGAA TGAGGAGGAG GATGACGTTC CTGaaatgaa 841 cagcagcttt acagccgatt ctggtgaaca agaggagcac actctccaag aaacagcatt 901 accacctgtg aatagtagca tcatcgctgc tcccatcacg gacccttctc agaagttccc 961 tcaataccta cctctttctg cagaggataa tttaggtcct ctacctgaaa actgggagat 1021 ggcctatact gaaaatggag aagtctattt tatagaccat aacacgaaaa caacatcttg 1081 gttagaccct cggtgcctaa acaagcagca gaagccactg gaagagtgtg aagatgatga 1141 aggggtacac accgaggagc tggacagtga actagaactg cctgctggtt gggaaaagat 1201 tgaagaccct gtctatggta tctactatgt agaccacatc aacaggaaga cacaatatga 1261 gaacccggtt ctagaagcca aacggaagaa gcagcttgag cagcagcagc agcagcaaca 1321 gcagcagcaa cagcagcagc agcagcagca gcagcagcag acagaagaat ggacagaaga 1381 tcactcagcc cttgtgcctc ctgttattcc aaaccaccct ccaagcaatc cagagccagc 1441 cagagaagtt ccacttcagg gcaaaccctt ttttacacga aacccttctg agttgaaagg 1501 caagttcatt cacacaaagc tgcggaaaag cagtcgtggc tttggcttca cggtggttgg 1561 aggggatgaa cctgatgagt ttctccagat caagagcttg gtcctagatg gtcctgctgc 1621 attggatggc aagatggaaa caggggatgt gattgtaagt gtgaatgaca cctgtgtttt 1681 gggacacaca catgctcaag ttgtgaagat cttccagtcc atccccattg gtgccagcgt 1741 ggaccttgaa ctctgccgag gttatccatt gccttttgat ccagatgacc ccaatacaag 1801 tttagtgacc tcggtagcca ttttggataa agaaccaatt attgtgaatg ggcaagagac 1861 ctatgattca ccagctagcc acagtagtaa aacaggcaaa gtcaatggca tgaaggatgc 1921 caggccaagc agcccagcgg acgtggcttc aaatagttct catggttatc ctaatgacac 1981 tgtttctttg gcttcctcca tagccactca gccagaactc ataactgttc atattgtcaa 2041 agggccaatg ggctttggtt ttactatcgc agacagtcct ggtgggggtg gccaaagagt 2101 gaaacagatt gttgacagcc caaggtgccg aggcctgaaa gaaggggatc tcatagtgga 2161 agttaataag aagaacgtgc aggccctaac tcacaaccaa gtggtggata tgctggttga 2221 gtgtcctaag ggaagtgagg tcacattgtt ggtgcaacga ggagggctgc cagttcccaa 2281 gaagagccca aagtcgcaac cactggaaag gaaagacagc cagaatagtt ctcagcacag 2341 tgtttccagc caccgaagcc tgcacacagc atccccaagc cacagcacac aggtgctccc 2401 cgagttccca cctgcagagg cccaagctcc agatcaaact gacagctctg gccagaaaaa 2461 accagatcct tttaaaatct gggcccagtc caggagcatg tatgaaaacc gacttccaga 2521 ttaccaggaa caggacatct tcctctggag aaaagagact ggatttggat ttaggattct 2581 gggtggaaat gaaccagggg aacctattta tattggtcac atcgtaccac tgggtgctgc 2641 tgatactgac ggccgcctga ggtctggaga tgaattaatc tgtgtggatg ggacgccagt 2701 aattggaaaa tcacaccagc ttgtggtcca gcttatgcaa caagctgcca agcaaggcca 2761 cgtcaatctc acggtgcggc gtaaagtggt ttttgcggtg cccaaaaccg agaacgaggt 2821 gccctcgcca gcctcctctc atcacagtag caaccagccg gcctcgctga cagaagagaa 2881 gcgcactccg cagggcagcc agaactcgct gaacacggtg agctcgggca gcggcagcac 2941 cagcggcatc ggcagtggcg gcggcggggg cagcggcgtg gtcagcaccg tggtgcagcc 3001 ctacgacgtg gagatccggc gcggggagaa cgagggcttc ggcttcgtca tcgtgtcctc 3061 ggtgagcagg cccgaagcag gcacgacctt tggcaatgca tgtgtggcta tgcctcacaa 3121 aataggtcgg attattgagg ggagccctgc tgaccgctgt ggcaagctga aagtaggaga 3181 ccggatcttg gcagtaaatg gatgttccat caccaacaaa tcccattcag acattgtgaa 3241 cctaatcaag gaagcgggaa acacagttac cctccgcatc attcctgggg atgagtcctc 3301 gaatgccacc ttgctgacca atgcagagaa gattgccacc atcaccacca cacacacccc 3361 ttctcagcaa gggacccagg agacaaggaa taccaccaaa ccaaagcagg aatctcaatt 3421 tgagttcaaa gcaccccaag caacacagga gcaagatttt tacactgtgg aactggaaag 3481 aggagccaag ggatttggct ttagccttcg aggaggccga gagtataaca tggacctcta 3541 tgttctgcgc ttagcagagg acggtcctgc ggagaggtgt ggaaagatga ggattggtga 3601 tgaaatttta gagatcaatg gtgagaccac caaaaacatg aagcattctc gagctataga 3661 actgattaag aatggtggcc gcagagttcg tctgtttctg aagcggggag acggctcagt 3721 accagaTTAT GACCCCAGCA GCGACCGCCA CGGCCCCGCC ACCGGTCCAC AAGGTGTTCC 3781 GAAGTGAGGC CGGGCCGACC GCGGCAGCAT CGTCATTGGA GTCCAGTTAC CCACCCGATC 3841 TTCACAAATC ATCACCACAT GGGGAAAAGC GGGCACACGC AAGGGATCCG AAAGGCAGCA 3901 GAGAGTATAG CAGACAACCC AATGAACACC ACACCTGGAA TGGGACTTCG AGGAAACCCG 3961 ACAGCGGGGC ATGCCGACCC AAGGACCGGG CGCCGGAGGG ACGGAGGGAC GCGCAGGCCG 4021 AGCGCGCGGC AGCCGCCAAC GGCCCCAAGA GGCGGTCCCC AGAGAAGCGG AGGGAGGGCA 4081 CCCGCAGCGC CGACAACACT TTGGAGAGGA GGGAGAAGCA CGAGAAGAGA CGAGACGTCT 4141 CTCCGGAGCG GAGGCGAGAG CGGTCACCCA CCCGCAGGAG AGACGGCTCC CCCAGCCGGC 4201 GGAGACGGTC TCTGGAGAGA CTCCTGGAGC AGAGGAGGTC CCCCGAGCGC AGGAGAGGGG 4261 GCTCGCCCGA GCGCAGGGCC AAGTCCACCG ACCGGAGGCG CGCACGCTCC CCCGAGCGCA 4321 GGAGAGAGCG GTCCCTGGAC AAAAGGAACA GAGAGGACAG AGCCAGCCAC CGAGAAAGGG 4381 AAGAGGCGAA TCTGAAACAG GATGCCGGCA GAAGTTCCAG ACATCCCCCG GAGCAGAGAA 4441 GGCGACCTTA CAAAGAATGT AGCACCGACC TCAGTATCTT GCCAACTTTC TTGTACAAAG 4501 TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACGATCAGTC 4561 AAAATAAAAT CATATAGCCA TCCAGCGATC CTCGC