Construct: ORF ccsbBroadEn_14945

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006273.1_s300c1, BRDN0000457477
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KNTC1 (9735)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9735 KNTC1 kinetochore associated 1 NM_014708.6 99.8% 43.5% (many diffs)
2 human 9735 KNTC1 kinetochore associated 1 XM_006719706.2 99.8% 43.5% (many diffs)
3 human 9735 KNTC1 kinetochore associated 1 XM_011539027.3 98.7% 44% (many diffs)
4 human 9735 KNTC1 kinetochore associated 1 XM_017020254.2 98.5% 44.1% (many diffs)
5 human 9735 KNTC1 kinetochore associated 1 XM_017020255.2 93% 36.6% (many diffs)
6 human 9735 KNTC1 kinetochore associated 1 XM_017020256.2 73.5% 17.1% (many diffs)
7 human 9735 KNTC1 kinetochore associated 1 XM_011539029.3 70.2% 61.8% (many diffs)
8 human 9735 KNTC1 kinetochore associated 1 XR_944845.3 67.1% (many diffs)
9 human 9735 KNTC1 kinetochore associated 1 XR_944844.3 67% (many diffs)
10 human 9735 KNTC1 kinetochore associated 1 XM_011539030.1 66.1% 65.4% (many diffs)
11 human 9735 KNTC1 kinetochore associated 1 XM_024449285.1 65.7% 66.1% (many diffs)
12 human 9735 KNTC1 kinetochore associated 1 XM_017020257.1 65.4% 65.6% (many diffs)
13 mouse 208628 Kntc1 kinetochore associated 1 XM_006530251.3 51.6% 6.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2958
ORF length:
2889
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GTGGAATGAT ATTGAGCTGC TAACAAATGA TGATACCGGA AGTGGGTACC
121TGAGTGTCGG TTCAAGAAAA GAACATGGAA CTGCTTTATA TCAAGTAGAT TTGCTAGTGA
181AGATCTCTTC TGAAAAGGCC TCATTAAATC CAAAGATACA GGCATGCAGC TTAAGTGATG
241GGTTTATTAT TGTAGCCGAC CAATCAGTGA TATTGCTTGA CAGTATTTGT AGATCACTTC
301AATTGCATCT TGTCTTTGAT ACTGAAGTGG ATGTAGTTGG CCTTTGTCAA GAAGGAAAGT
361TTCTTTTGGT TGGCGAGAGA AGTGGCAACC TACATCTTAT TCATGTAACA TCAAAACAAA
421CACTACTCAC TAATGCATTT GTTCAGAAAG CTAACGATGA AAATCGGCGG ACTTACCAGA
481ATCTTGTCAT TGAGAAGGAT GGTTCAAATG AAGGTACCTA TTATATGCTA CTTCTTACAT
541ACAGTGGATT TTTTTGTATT ACAAACCTTC AGCTTTTAAA AATTCAACAA GCAATTGAGA
601ATGTAGACTT CAGTACAGCA AAAAAGTTAC AAGGACAAAT CAAGTCCAGT TTTATTTCTA
661CTGAAAATTA TCATACTCTT GGTTGTCTCA GTCTTGTGGC TGGAGATTTA GCAAGTGAAG
721TTCCTGTGAT AATTGGGGGA ACCGGTAATT GTGCATTCTC AAAATGGGAA CCAGATTCTT
781CCAAGAAAGG AATGACAGTT AAGAACCTTA TTGATGCAGA GATTATTAAA GGTGCAAAGA
841AGTTCCAGCT GATAGACAAT CTACTTTTTG TTCTTGATAC TGATAACGTG CTGAGTTTAT
901GGGATATTTA CACTCTAACT CCTGTATGGA ACTGGCCCTC TCTTCACGTA GAAGAGTTTC
961TTCTTACTAC AGAAGCAGAC TCTCCTTCAT CAGTCACGTG GCAAGGAATT ACAAATCTCA
1021AATTAATAGC TCTGACAGCT TCAGCTAATA AGAAGATGAA AAACCTCATG GTTTATTCAT
1081TACCTACAAT GGAAATACTA TATTCTTTGG AAGTATCTAG TGTTTCTTCT CTGGTCCAAA
1141CAGGAATTAG CACAGATACC ATATACCTTT TAGAAGGAGT TTGCAAAAAT GATCCAAAAT
1201TGTCTGAAGA CTCAGTCTCT GTGTTAGTAC TCAGATGTCT TACGGAAGCT TTACCAGAAA
1261ACAGATTGAG TCGGTTACTT CACAAACACA GATTTGCTGA AGCTGAGAGT TTTGCCATTC
1321AGTTTGGACT AGATGTTGAG CTTGTTTACA AGGTCAAGTC AAATCATATA TTGGAGAAAC
1381TGGCATTGAG TTCTGTGGAT GCCAGTGAAC AGACCGAATG GCAACAACTT GTAGACGACG
1441CTAAGGAAAA TCTACATAAG ATCCAGGATG ATGAATTTGT GGTGAATTAC TGCCTGAAAG
1501CTCAGTGGAT AACCTATGAA ACCACTCAAG AGATGCTGAA TTATGCCAAA ACCAGGCTTT
1561TGAAGAAAGA AGATAAAACT GCTCTCATTT ATTCTGATGG CTTGAAAGAG GTGCTAAGAG
1621CTCATGCAAA ATTGACTACT TTTTATGGAG CATTTGGACC AGAAAAATTC AGTGGCAGTT
1681CTTGGATTGA ATTTCTAAAT AATGAAGATG ATCTTAAAGA TATTTTTTTA CAGCTAAAAG
1741AAGGAAACCT TGTTTGTGCA CAGTATCTTT GGCTTCGACA TCGGGCAAAC TTTGAAAGCA
1801GATTTGATGT GAAAATGCTG GAGAGCTTGC TCAACTCAAT GTCTGCATCA GTCTCTTTGC
1861AAAAGCTGTG TCCATGGTTT AAAAATGATG TGATTCCATT TGTAAGAAGG ACTGTGCCTG
1921AAGGACAGAT AATTCTTGCA AAATGGTTGG AACAAGCAGC CAGGAACCTT GAATTAACTG
1981ATAAGGCAAA TTGGCCAGAA AATGGACTTC AATTGGCAGA GATATTTTTT ACAGCAGAAA
2041AAACAGACGA GTTGGGATTG GCATCTTCCT GGCATTGGAT TTCCTTGAAA GATTATCAGA
2101ACACAGAGGA AGTATGTCAG CTAAGGACTT TGGTAAATAA CTTGCGAGAG TTGATCACGT
2161TGCATAGGAA GTACAACTGC AAATTAGCCC TCTCTGATTT TGAGAAGGAA AATACAACCA
2221CCATAGTGTT CCGAATGTTT GATAAAGTGC TGGCCCCAGA GCTTATTCCC TCCATCTTAG
2281AGAAGTTTAT AAGAGTTTAC ATGAGAGAAC ATGACTTGCA AGAGGAGGAA CTTCTCTTGC
2341TGTACATAGA GGATTTACTG AATAGATGCA GCTCAAAGTC CACATCACTC TTTGAAACAG
2401CATGGGAAGC AAAGGCCATG GCAGTAATAG CGTGTTTATC TGACACGGAC CTCATATTTG
2461ATGCCGTGCT CAAGATCATG TATGCGGCAG TGGTTCCTTG GAGTGCAGCT GTGGAGCAAC
2521TGGTGAAACA GCACCTGGAA ATGGACCATC CCAAAGTCAA GTTATTACAG GAAAGTTACA
2581AACTAATGGA GATGAAAAAA CTTTTACGAG GCTATGGAAT AAGAGAGGTA AATCTCTTAA
2641ACAAGGAAAT AATGAGAGTG GTTAGATACA TTCTCAAACA AGATGTCCCA TCTTCTTTAG
2701AAGATGCTTT AAAGGTAGCC CAAGCGTTTA TGTTATCTGA TGATGAGATC TACAGTCTAA
2761GAATTATTGA CCTGATTGAT AGAGAACAGG GTGAAGACTG TCTCCTTCTG TTGAAGTCTT
2821TGCCTCCTGC TGAAGCTGAG AAAACTGCAG AAAGAGTCAT CATATGGGCA CGACTGGCAT
2881TACAAGAAGA GCCAGATCAT TCTAAAGAGG GCAAGGCCTG GAGAATGTCT GTAGCGAAGA
2941CATCCGTGGA TCATTCTTAA GATACTATGT GACATTCAGA AAGACAATCT GCAGAAGAAG
3001GACGAAACGT GAAAAAatgt tgaaactatt taaagaggtt gctagcttac aggagaactt
3061tgaggtcttt ctttcatttg aagattatag caatagttcc ctggtagcag atctccgtga
3121gcagcacatt aaagctcacg aagttgcaca ggcgaaacac aaacctggga gcaccccaga
3181gcccatagct gctgaggtga ggagcccaag catggaatca aagctgcaca gacaggcact
3241ggccctgcag atgtccaaac aagagctgga ggcagagctg accttgagag ccttaaaaga
3301tgggaacatc aaaacagcac tgaaaaaatg cagcgacttg tttaagtatc actgcaatgc
3361tgacactggg aaattgctat ttctgacatg tcagaagctt tgtcagatgt tggctgataa
3421tgtcccagtg acagtgcctg tgggactgaa tcttccttcc atgatacatg atctagcaag
3481ccaagctgcc accatttgca gtccagattt tttactagat gctttagaac tatgtaaaca
3541tactttaatg gctgtagagc tttccagaca atgccaaatg gatgactgtg gaatcctcat
3601gaaagcttct tttgggacac ataaagatcc atatgaagag tggtcttaca gtgacttctt
3661cagtgaagat ggaattgttc ttgagtcaca gatggtgctt ccagtgattt atgaactgat
3721ttcatctctt gtgcctctag ctgaaagcaa gagatatccc ttggagtcta ccagtttgcc
3781atactgctcc cttaatgaag gagatggcct tgttttacct gttataaatt ccatctctgc
3841cctgcttcag aatcttcagg aatctagcca gtgggagcta gccctaagat ttgtggttgg
3901ttcatttggt acctgtcttc agcactctgt gtcaaacttc atgaatgcca ctttgagtga
3961aaagttattt ggagagacta cattagttaa atcaaggcat gttgttatgg aattgaaaga
4021aaaagctgtt atatttatca gggaaaatgc tacaacacta ctgcacaaag tatttaattg
4081tcgcttggta gatcttgacc tggcgttggg ttactgcact ctcttacctc aaaaagatgt
4141gtttgaaaat ctctggaagc tcatagataa agcatggcag aattacgaca aaatcttggc
4201aatatctctg gtgggctctg agctggcaag tctctatcag gaaatagaaa tggggcttaa
4261gttccgtgaa ctcagtactg atgcccagtg gggcattcgt cttggtaaac ttggtatttc
4321ttttcaacca gttttcaggc aacattttct caccaagaaa gacctcatta aagctcttgt
4381ggagaatata gatatggaca caagcctcat tttggaatat tgcagcacat ttcagttgga
4441ctgcgatgca gttcttcagc tcttcattga aacgctgctc cacaacacaa atgccggcca
4501aggccaggga gatgcaagca tggactctgc aaagcggcgg catcccaaac tcctggccaa
4561agcccttgag atggttcctt tactgacgag cacaaaagat ttggtcatca gtcttagtgg
4621aatactacat aagctggatc cttatgacta tgaaatgatt gaagttgtct tgaaagttat
4681agaacgagct gatgaaaaga taaccaatat taatattaat caggcattga gtattctgaa
4741acatttgaag tcatacagaa gaatttctcc tcccgtggat ctagaatatc agtatatgtt
4801ggaacatgtc ataactttgc catcagctgc ccaaactaga ctgccttttc acctgatatt
4861ctttggcaca gcacagaact tctggaaaat tctctctaca gaactcagtg aagaatcttt
4921cccaacattg ctcttaattt cgaaattaat gaagttctct ctggacactc tgtacgtgtc
4981tacagcaaaa cacgttttcg aaaaaaaact gaagccaaag ctcctgaagt taacacaagc
5041taaatcctca acactgatta acaaggaaat aactaagatc acgcagacca tcgaatcctg
5101cttactctct atagtcaacc cagagtgggc tgtagctatt gccatcagcc ttgcccagga
5161tatccctgaa ggttccttca agatatctgc tttgaaattc tgcctttatt tagctgagag
5221atggctacag aatatcccat cgcAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA
5281GAAGCTTCNN ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT
5341GAACACTGAG GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA
5401CGAACATCCT AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT
5461TCATGCAGCA GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA
5521CATGTTGTTG GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT
5581ATTTGAACTT CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC
5641AATTGATTAT AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG
5701TATGCATCAG TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC
5761TGACAAGGAA ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT
5821GAGATGTATA ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT
5881ATTTTGCAGC AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA
5941GTCCATGGCA GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA
6001GCTTTGGAAT GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA
6061AGTTTTAAAA GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC
6121GTGGCAGCGT GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA
6181GCTGTCAGAT TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT
6241TGACCTGATC GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC
6301TTGTCTGATG CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG
6361TTCCTGTGAC CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT
6421AGCAGGATTT TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA
6481GTTTGGGATT TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC
6541CAACAATATC ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC
6601AGTCTTGATA ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC
6661CTGTGAAATT CTGAAATGTT TCTTAGTGGA TTATCGTTGC CAACTTTCTT GTACAAAGTT
6721GGcattataa gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)