Construct: ORF ccsbBroadEn_14951

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018884.1_s300c1, BRDN0000458180
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
GNE (10020)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10020 GNE glucosamine (UDP-N-acetyl)-... NM_005476.7 99.4% 33.7% (many diffs)
2 human 10020 GNE glucosamine (UDP-N-acetyl)-... XM_017014167.1 99.4% 33.7% (many diffs)
3 human 10020 GNE glucosamine (UDP-N-acetyl)-... NM_001128227.3 95.3% 32.4% (many diffs)
4 human 10020 GNE glucosamine (UDP-N-acetyl)-... NM_001190388.1 93.4% 27.1% (many diffs)
5 human 10020 GNE glucosamine (UDP-N-acetyl)-... XM_017014168.1 92.4% 31.4% (many diffs)
6 human 10020 GNE glucosamine (UDP-N-acetyl)-... NM_001190383.3 89.6% 37.6% 733delA;1374A>C;1409_1410ins222
7 human 10020 GNE glucosamine (UDP-N-acetyl)-... XM_005251334.4 88.6% 30% (many diffs)
8 human 10020 GNE glucosamine (UDP-N-acetyl)-... NM_001190384.3 84.2% 22.7% (many diffs)
9 mouse 50798 Gne glucosamine (UDP-N-acetyl)-... NM_001190414.1 87.1% 33.5% (many diffs)
10 mouse 50798 Gne glucosamine (UDP-N-acetyl)-... XM_006538073.3 84.2% 32.3% (many diffs)
11 mouse 50798 Gne glucosamine (UDP-N-acetyl)-... NM_015828.3 83.5% 32.1% (many diffs)
12 mouse 50798 Gne glucosamine (UDP-N-acetyl)-... XM_006538074.3 80.5% 25.4% (many diffs)
13 mouse 50798 Gne glucosamine (UDP-N-acetyl)-... XM_017320310.1 62.7% 5.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
92
ORF end:
833
ORF length:
741
Sequence:
1TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT
61AATGCCAACT TTGTACAAAA AAGTTGGCAC CATGGAGAAG AATGGAAATA ACCGAAAGCT
121GCGGGTTTGT GTTGCTACTT GTAACCGTGC AGATTATTCT AAACTTGCCC CGATCATGTT
181TGGCATTAAA ACCGAACCTG AGTTCTTTGA ACTTGATGTT GTGGTACTTG GCTCTCACCT
241GATAGATGAC TATGGAAATA CATATCGAAT GATTGAACAA GATGACTTTG ACATTAACAC
301CAGGCTACAC ACAATTGTGA GGGGAGAAGA TGAGGCAGCC ATGGTGGAGT CAGTAGGCCT
361GGCCCTAGTG AAGCTGCCAG ATGTCCTTAA TCGCCTGAAG CCTGATATCA TGATTGTTCA
421TGGAGACAGG TTTGATGCCC TGGCTCTGGC CACATCTGCT GCCTTGATGA ACATCCGAAT
481CCTTCACATT GAAGGTGGGG AAGTCAGTGG GACCATTGAT GACTCTATCA GACATGCCAT
541AACAAAACTG GCTCATTATC ATGTGTGCTG CACCCGCAGT GCAGAGCAGC ACCTGATATC
601CATGTGTGAG GACCATGATC GCATCCTTTT GGCAGGCTGC CCTTCCTATG ACAAACTTCT
661CTCAGCCAAG AACAAAGACT ACATGAGCAT CATTCGCATG TGGCTAGGTG ATGATGTAAA
721ATCTAAAGAT TACATTGTTG CACTACAGCA CCCTGTGACC ACTGACATTA AGCATTCCAT
781AAAAATGTTT GAATTAACAT TGGATGCACT TATCTCATTT AACAGCGGAC CCTAGTCCTG
841TTTCCAAATA TTGACGCAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGaa gaagggcatt
901gagcatcatc ccaactttcg tgcagttaaa cacgtcccat ttgaccagtt tatacagttg
961gttgcccatg ctggctgtat gattgggaac agcagctgtg gggttcgaga agttggagct
1021tttggaacac ctgtgatcaa cctgggaaca cgtcagattg gaagagaaac aggggagaat
1081gttcttcatg tccgggatgc tgacacccaa gacaaaatat tgcaagcact gcaccttcag
1141tttggtaaac agtacccttg ttcaaagata tatggggatg gaaatgctgt tccaaggatt
1201ttgaagtttc tcaaatctat cgatcttcaa gagccactgc aaaagaaatt ctgctttcct
1261cctgtgaagg agaatatctc tcaagatatt gaccatattc ttgaaactct aagtgccttg
1321gccgttgatc ttggcgggac gaacctccga gttgcaatag tcagcatgaa gggtgaaata
1381gttaagaagt atactcagtt caatcctaaa acctatgaag agaggattaa tttaatccta
1441cagatgtgtg tggaagctgc agcCGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA
1501GGCATTTCCA CAGGTGNCCG TGTAATCTTC GGAAAAGGAA ATGTGCTGCA TTCAACCCAA
1561CTGATCCAAG AGTGGAACTC TGTGACNTTT AGGACCCCCC TTTCTGACAC TTTGCATCTC
1621CCTGTGTGGG TAGACAATGG TGGCAACTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA
1681GGAAAGGGAC TGGAAAACTT TGTTACACTT ATCACAGGCA CAGGAATCGG TGGTGGAATT
1741ATCCATCAGC ATGAATTGAT CCACGGAAGC TCCTTCTGTG CTGCAGAACT GGGCCACCTT
1801GTTGTGTCTC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ATGGGTGCAT TGAAGCATAC
1861GCCTCTGGAA TGGCCTTGCA GAGGGAGGCA AAAAAGCTCC ATGATGAGGA CCTGCTCTTG
1921GTGGAAGGGA TGTCAGTGCC AAAAGATGAG GCTGTGGGTG CGCTCCATCT CATCCAAGCT
1981GCGAAACTTG GCAATGCGAA GGCCCAGAGC ATCCTAAGAA CAGCTGGAAC AGCTTTGGGT
2041CTTGGGGTTG TGAACATCCT CCATACCATG AATCCCTCCC TTGTGATCCT CTCCGGAGTC
2101CTGGCCAGTC ACTATATCCA CATTGTCAAA GACGTCATTC GCCAGCAGGC CTTGTCCTCC
2161GTGCAGGACG TGGATGTGGT GGTTTCGGAT TTGGTTGACC CCGCCCTGCT GGGTGCTGCC
2221AGCATGGTTC TGGACTACAC AACACGCAGG ATCTACTTGC CAACTTTCTT GTACAAAGTT
2281GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA
2341AATAAAATCA TTATTGCCAT CCCAGCCTGA TT

Download FASTA (ORF) (Full)