Transcript: Human NM_005476.7

Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
GNE (10020)
Length:
5263
CDS:
171..2339

Additional Resources:

NCBI RefSeq record:
NM_005476.7
NBCI Gene record:
GNE (10020)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145858 ACATCAAGTTCAAAGAACTC pXPR_003 AGG 111 5% 2 0.5113 GNE GNE 77830
2 BRDN0001149214 CCAGGCTACACACAATTGTG pXPR_003 AGG 225 10% 3 0.2759 GNE GNE 77831
3 BRDN0001145959 ACATGAGCATCATTCGCATG pXPR_003 TGG 606 28% 3 -0.0534 GNE GNE 77832
4 BRDN0001149237 TGCTGACTATTGCAACTCGG pXPR_003 AGG 1259 58% 7 -0.1248 GNE GNE 77833
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005476.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000350446 TTGTAACCGTGCAGATTATTC pLKO_005 218 CDS 100% 13.200 18.480 N GNE n/a
2 TRCN0000356582 ACGAACCTCCGAGTTGCAATA pLKO_005 1419 CDS 100% 10.800 15.120 N GNE n/a
3 TRCN0000195274 CTACCAAGTTTCATACCAAAT pLKO.1 5051 3UTR 100% 10.800 15.120 N GNE n/a
4 TRCN0000006169 GCAGTTAAACACGTCCCATTT pLKO.1 1002 CDS 100% 10.800 15.120 N GNE n/a
5 TRCN0000356583 CCGATCATGTTTGGCATTAAA pLKO_005 249 CDS 100% 15.000 12.000 N GNE n/a
6 TRCN0000196714 GTACCCTTGTTCAAAGATATA pLKO.1 1232 CDS 100% 0.000 0.000 N GNE n/a
7 TRCN0000315398 GTACCCTTGTTCAAAGATATA pLKO_005 1232 CDS 100% 0.000 0.000 N GNE n/a
8 TRCN0000350521 TGCCAAAGCAGCTACAATAAT pLKO_005 2600 3UTR 100% 15.000 10.500 N GNE n/a
9 TRCN0000195334 CCCAACTTTCGTGCAGTTAAA pLKO.1 990 CDS 100% 13.200 9.240 N GNE n/a
10 TRCN0000006171 CCCGATCATGTTTGGCATTAA pLKO.1 248 CDS 100% 13.200 9.240 N GNE n/a
11 TRCN0000195218 CTGGTTATTGTTCAGACATTC pLKO.1 4893 3UTR 100% 10.800 7.560 N GNE n/a
12 TRCN0000196749 GATGACCGTTTCTTAACAATC pLKO.1 2406 3UTR 100% 10.800 7.560 N GNE n/a
13 TRCN0000006170 GCCAGTCACTATATCCACATT pLKO.1 2184 CDS 100% 4.950 3.465 N GNE n/a
14 TRCN0000006168 GCCTTCTTAGAACTCTGCTTA pLKO.1 2630 3UTR 100% 4.950 3.465 N GNE n/a
15 TRCN0000350523 ACCTATGAAGAGAGGATTAAT pLKO_005 1491 CDS 100% 15.000 9.000 N GNE n/a
16 TRCN0000006172 GCTGCATTCAACCAAACTGAT pLKO.1 1625 CDS 100% 4.950 2.970 N GNE n/a
17 TRCN0000156198 CCTCTTGAGTAGCTGGGATTA pLKO.1 4480 3UTR 100% 10.800 5.400 Y MRPL49 n/a
18 TRCN0000276507 CCTCTTGAGTAGCTGGGATTA pLKO_005 4480 3UTR 100% 10.800 5.400 Y MRPL49 n/a
19 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2752 3UTR 100% 4.950 2.475 Y ERAP2 n/a
20 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2753 3UTR 100% 13.200 6.600 Y LIAS n/a
21 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 3254 3UTR 100% 10.800 5.400 Y SMIM11A n/a
22 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4619 3UTR 100% 5.625 2.813 Y KLHL30 n/a
23 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3351 3UTR 100% 4.950 2.475 Y KAAG1 n/a
24 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4619 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005476.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02293 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02293 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478834 GGGAACACCACAAGTGTTGCTTTT pLX_317 18.4% 100% 100% V5 n/a
4 ccsbBroadEn_14951 pDONR223 73.7% 99.4% 33.7% None (many diffs) n/a
5 ccsbBroad304_14951 pLX_304 0% 99.4% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000474395 GCCAGAGTCTAAGCCCTCGGCCAA pLX_317 17.9% 99.4% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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