Construct: ORF ccsbBroadEn_14970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018858.1_s300c1, BRDN0000458154
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMKK2 (10645)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_006384.4 | 100% | 100% | |
| 2 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_001277764.1 | 82.6% | 82.6% | 87_206del |
| 3 | human | 10519 | CIB1 | calcium and integrin binding 1 | XM_006720375.2 | 71.8% | 70.4% | (many diffs) |
| 4 | human | 10519 | CIB1 | calcium and integrin binding 1 | NR_102428.1 | 63% | (many diffs) | |
| 5 | human | 10519 | CIB1 | calcium and integrin binding 1 | NR_102427.1 | 55.2% | (many diffs) | |
| 6 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_011870.5 | 88.9% | 93.7% | (many diffs) |
| 7 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_001291275.1 | 86.6% | 91% | (many diffs) |
| 8 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_001291276.1 | 67.7% | 72.2% | (many diffs) |
| 9 | mouse | 23991 | Cib1 | calcium and integrin bindin... | XM_011250861.2 | 54.9% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 199
- ORF end:
- 772
- ORF length:
- 573
- Sequence:
-
1 NNNNNANTNT NNNTNNANTN NNNNNNNNNT TTAAAATTCA GGAAACATCC CAGTCCCCGA 61 CGTGCAAATC GACTGTCTAG TCTTACGCTC GGGCCCCAAA TAATGATTTT ATTTTGACTG 121 ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG 181 TACAAAAAAG TTGGCACCAT GGGGGGCTCG GGCAGTCGCC TGTCCAAGGA GCTGCTGGCC 241 GAGTACCAGG ACTTGACGTT CCTGACGAAG CAGGAGATCC TCCTAGCCCA CAGGCGGTTT 301 TGTGAGCTGC TTCCCCAGGA GCAGCGGAGC GTGGAGTCGT CACTTCGGGC ACAAGTGCCC 361 TTCGAGCAGA TTCTCAGCCT TCCAGAGCTC AAGGCCAACC CCTTCAAGGA GCGAATCTGC 421 AGGGTCTTCT CCACATCCCC AGCCAAAGAC AGCCTTAGCT TTGAGGACTT CCTGGATCTC 481 CTCAGTGTGT TCAGTGACAC AGCCACGCCA GACATCAAGT CCCATTATGC CTTCCGCATC 541 TTTGACTTTG ATGATGACGG AACCTTGAAC AGAGAAGACC TGAGCCGGCT GGTGAACTGC 601 CTCACGGGAG AGGGCGAGGA CACACGGCTT AGTGCGTCTG AGATGAAGCA GCTCATCGAC 661 AACATCCTGG AGGAGTCTGA CATTGACAGG GATGGAACCA TCAACCTCTC TGAGTTCCAG 721 CACGTCATCT CCCGTTCTCC AGACTTTGCC AGCTCCTTTA AGATTGTCCT GTTGCCAACT 781 TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG 841 GTCACTATCA GTCAAAATAA AATCATTATT NNCNNNCNNN NNNNNANNNC CGATAGGTTG 901 AGTCGTATTA CATGGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCTACTTTTT 961 CTTTGAGGTA TGACAAAAGT GTGACTCAGT CAGGCTGTCT GTA