Construct: ORF ccsbBroadEn_15025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017687.1_s300c1, BRDN0000391313
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSTYK (25778)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_015375.3 | 99.7% | 99.5% | 1921T>C;2470_2473delAAGTinsGCTC;2600_2601delGGinsCA |
| 2 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509392.2 | 98.7% | 98.6% | (many diffs) |
| 3 | human | 25778 | DSTYK | dual serine/threonine and t... | NM_199462.3 | 95.1% | 95% | 1921T>C;2463_2464ins135 |
| 4 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509393.2 | 77.4% | 76.4% | (many diffs) |
| 5 | human | 25778 | DSTYK | dual serine/threonine and t... | XM_011509394.2 | 76.7% | 76.1% | (many diffs) |
| 6 | mouse | 213452 | Dstyk | dual serine/threonine and t... | NM_172516.4 | 88.2% | 91% | (many diffs) |
| 7 | mouse | 213452 | Dstyk | dual serine/threonine and t... | XM_006529356.3 | 78.8% | 82.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2856
- ORF length:
- 2787
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGNNNCAT GGAGGGCGAC GGGGTGCCAT GGGGCAGCGA GCCCGTCTCG GGTCCCGGCC 121 CCGGCGGCGG CGGAATGATC CGCGAGCTGT GCCGGGGCTT CGGCCGCTAC CGCCGCTACC 181 TGGGACGGCT GCGACAGAAC CTGCGCGAGA CCCAGAAGTT CTTCCGCGAC ATCAAGTGCT 241 CCCACAACCA CACTTGTCTC TCCTCCCTCA CGGGCGGCGG CGGGGCCGAG CGCGGCCCTG 301 CAGGCGATGT CGCCGAAACC GGGCTGCAGG CGGGCCAACT GAGCTGCATT TCCTTCCCAC 361 CTAAGGAAGA GAAGTACCTC CAGCAGATTG TGGACTGCCT CCCTTGCATA CTGATCCTCG 421 GCCAGGATTG TAACGTCAAG TGCCAGCTGT TGAATCTGCT GTTGGGGGTG CAGGTGCTTC 481 CCACCACCAA GCTGGGCAGT GAGGAGAGCT GTAAGCTTCG GCGCCTCCGC TTCACCTATG 541 GGACTCAGAC TCGGGTCAGC CTGGCGCTCC CTGGACAGTA TGAACTAGTG CACACGCTGG 601 TTGCTCATCA GGGCAACTGG GAGACCATCC CTGAGGAGGA TCTGGAGGTC CAAGAGAACA 661 ATGAGGATGC TGCTCATGTT TTAGCGGAAC TGGAGGTAAC GATGCACCAT GCTCTCTTAC 721 AGGAAGTGGA CGTTGTGGTA GCACCATGCC AAGGCCTCCG GCCCACAGTG GATGTTCTGG 781 GTGACTTGGT GAATGATTTC TTGCCTGTGA TAACCTATGC ACTCCACAAA GATGAACTCT 841 CTGAGAGGGA TGAGCAAGAG CTTCAGGAAA TCCGAAAGTA TTTCTCCTTT CCTGTATTCT 901 TTTTCAAAGT GCCGAAACTG GGCTCGGAGA TAATAGACTC CTCAACCAGG AGAATGGAGA 961 GCGAAAGATC ACCGCTTTAT CGCCAGCTAA TTGACCTGGG CTATCTGAGC AGCAGTCACT 1021 GGAACTGTGG GGCTCCTGGC CAGGATACTA AAGCTCAGAG CATGTTGGTG GAACAGAGTG 1081 AAAAGCTGAG ACACTTGAGC ACATTTTCTC ACCAGGTGTT ACAGACTCGC CTGGTGGATG 1141 CAGCCAAGGC CCTGAACCTG GTGCACTGCC ACTGCCTTGA CATCTTTATT AACCAGGCAT 1201 TTGACATGCA GCGGGACCTG CAGATCACTC CCAAACGTCT GGAATATACT CGAAAAAAGG 1261 AGAATGAGTT GTATGAATCA TTGATGAATA TTGCCAACCG AAAGCAGGAG GAAATGAAGG 1321 ATATGATTGT TGAGACACTT AATACCATGA AGGAGGAACT TCTGGATGAT GCTACTAACA 1381 TGGAGTTTAA AGACGTCATT GTCCCTGAGA ATGGAGAACC AGTAGGCACC AGAGAGATCA 1441 AATGCTGCAT CCGACAGATC CAGGAACTCA TCATCTCCCG ACTTAATCAG GCAGTGGCTA 1501 ATAAGCTGAT CAGCTCAGTG GATTACCTGA GGGAAAGCTT CGTCGGAACC CTGGAACGAT 1561 GTCTGCAGAG CCTGGAGAAG TCTCAGGATG TCTCAGTTCA CATCACCAGT AATTATCTCA 1621 AACAGATCTT AAATGCTGCC TATCATGTTG AAGTCACGTT TCACTCAGGG TCGTCAGTTA 1681 CAAGGATGCT ATGGGAGCAA ATCAAACAGA TCATCCAGCG CATCACATGG GTGAGCCCAC 1741 CTGCCATCAC TCTGGAATGG AAGAGGAAGG TGGCCCAGGA AGCCATTGAG AGCCTCAGCG 1801 CCTCCAAATT GGCTAAGAGC ATTTGCAGCC AATTCCGGAC TCGGCTCAAT AGTTCCCACG 1861 AGGCTTTTGC AGCCTCCTTG CGGCAGCTGG AAGCTGGCCA CTCAGGCCGG TTAGAGAAAA 1921 CGGAAGATCT ATGGCTGAGG GTTCGGAAAG ATCATGCTCC CCGCCTGGCC CGCCTTTCTC 1981 TGGAAAGCCG TTCTTTACAG GATGTCTTGC TTCATCGTAA ACCTAAACTG GGACAGGAAC 2041 TGGGCCGGGG CCAGTATGGT GTGGTATACC TGTGTGACAA CTGGGGAGGA CACTTCCCTT 2101 GTGCCCTCAA ATCAGTTGTC CCTCCAGATG AGAAGCACTG GAATGATCTG GCTTTGGAAT 2161 TTCACTATAT GAGGTCTCTG CCGAAGCATG AGCGATTGGT GGATCTCCAT GGTTCAGTCA 2221 TTGACTACAA CTATGGTGGT GGCTCCAGCA TTGCTGTGCT CCTCATTATG GAGCGGCTAC 2281 ACCGGGATCT CTACACAGGG CTGAAGGCTG GGCTGACCCT GGAGACACGT TTGCAGATAG 2341 CACTAGATGT GGTGGAGGGA ATCCGCTTCC TGCACAGCCA GGGACTTGTC CATCGTGATA 2401 TCAAACTGAA AAATGTGCTG CTGGATAAGC AGAACCGTGC CAAGATCACT GACTTAGGAT 2461 TCTGCAAGCC AGAGGCCATG ATGTCAGGCA GCATTGTGGG GACACCAATC CATATGGCCC 2521 CTGAACTTTT CACAGGGGCT Cacgataatt ccgtggatgt ctacgctttt ggaattcttt 2581 tctggtatat ctgctcaggc tctgtcaagc tccctgaggc atttgagagg tgtgctagca 2641 aagaccatct ctggaacaat gtgcggACAG GGGCTCGCCC AGAACGTCTT CCTGTGTTTG 2701 ATGAGGAGTG CTGGCAGTTG ATGGAAGCCT GTTGGGATGG CGACCCCTTG AAGAGGCCTC 2761 TCTTGGGCAT TGTCCAGCCC ATGCTCCAGG GCATCATGAA TCGGCTCTGC AAGTCCAATT 2821 CTGAGCAGCC AAACAGAGGA CTAGATGATT CTACTTGCCC AACTTTCTTG TACAAAGTtg 2881 gcattataag aaagcattgc ttatcaattt gttgcaacga ac