Construct: ORF ccsbBroadEn_15077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018985.1_s300c1, BRDN0000458281
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKAG3 (53632)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | NM_017431.3 | 99.2% | 48.6% | (many diffs) |
| 2 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | XM_017004343.2 | 99.2% | 48.6% | (many diffs) |
| 3 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | NM_153744.3 | 84.9% | 35.3% | (many diffs) |
| 4 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495988.3 | 84.9% | 35.3% | (many diffs) |
| 5 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495989.3 | 84.9% | 35.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 129
- ORF end:
- 861
- ORF length:
- 732
- Sequence:
-
1 GGGGGGNGGG GGGCGTGAAT AGCTGGGCCC AATATGATTT TATTTTGACT GATAGTGACC 61 TGTTCGTTGC ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG 121 TTGGCACCAT GGAGCCCGGG CTGGAGCACG CACTGCGCAG GACCCCTTCC TGGAGCAGCC 181 TTGGGGGTTC TGAGCATCAA GAGATGAGCT TCCTAGAGCA AGAAAACAGC AGCTCATGGC 241 CATCACCAGC TGTGACCAGC AGCTCAGAAA GAATCCGTGG GAAACGGAGG GCCAAAGCCT 301 TGAGATGGAC AAGGCAGAAG TCGGTGGAGG AAGGGGAGCC ACCAGGTCAG GGGGAAGGTC 361 CCCGGTCCAG GCCAGCTGCT GAGTCCACCG GGCTGGAGGC CACATTCCCC AAGACCACAC 421 CCTTGGCTCA AGCTGATCCT GCCGGGGTGG GCACTCCACC AACAGGGTGG GACTGCCTCC 481 CCTCTGACTG TACAGCCTCA GCTGCAGGCT CCAGCACAGA TGATGTGGAG CTGGCCACGG 541 AGTTCCCAGC CACAGAGGCC TGGGAGTGTG AGCTAGAAGG CCTGCTGGAA GAGAGGCCTG 601 CCCTGTGCCT GTCCCCGCAG GCCCCATTTC CCAAGCTGGG CTGGGATGAC GAACTGCGGA 661 AACCCGGCGC CCAGATCTAC ATGCGCTTCA TGCAGGAGCA CACCTGCTAC GATGCCATGG 721 CAACTAGCTC CAAGCTAGTC ATCTTCGACA CCATGCTGGA GATCAAGAAG GCCTTCTTTG 781 CTCTGGTGGC CAACGGGGTG CGGGCAGCCC CTCTATGGGA CAGCAAGAAG CAGAGCTTTT 841 GTGGGGATGC TGACCATCAC TGACTTTTTC CTGgtgcGGC ATCGTTATTC CAGGTCCCCC 901 CTTGTCCCAG ATCTATGAGA TTGAACAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT 961 GCAAGGCTGC TTCAAGCTTC TGGTCTCCAT CTCTCCTAAT GATAGCCTGT TTGAAGCTGT 1021 CTACACCCTC ATCAAGAACC GGATCCATCG CCTGCCTGTT CTTGACCCGG TGTCAGGCAA 1081 CGTACTCCAC ATCCTCACAC ACAAACGCCT GCTCAAGTTC CTGCACATCT TTGGTTCCCT 1141 GCTGCCCCGG CCCTCCTTCC TCTACCGCAC TATCCAAGAT TTGGGCATCG GCACATTCCG 1201 AGACTTGGCT GTGGTGCTGG AGACAGCACC CATCCTGACT GCACTGGACA TCTTTGTGGA 1261 CCGGCGTGTG TCTGCACTGC CTGTGGTCAA CGAATGTGGT CAGGTCGTGG GCCTCTATTC 1321 CCGCTTTGAT GTGATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTGG ACATGAGTGT 1381 GGGAGAAGCC CTGAGGCAGA GGACACTATG TCTGGAGGGA GTCCTTTCCT GCCAGCCCCA 1441 CGAGAGCTTG GGGGAAGTGA TCGACAGGAT TGCTCGGGAG CAGGTACACA GGCTGGTGCT 1501 AGTGGACGAG ACCCAGCATC TCTTGGGCGT GGTCTCCCTC TCCGACATCC TTCAGGCACT 1561 GGTGCTCAGC CCTGCTGGCA TCGATGCCCT CGGGGCCTTG CCAACTTTCT TGTACAAAGT 1621 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 1681 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 1741 CATAGCTGTT TCCC