Transcript: Mouse XM_006495989.3

PREDICTED: Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Prkag3 (241113)
Length:
3145
CDS:
419..1888

Additional Resources:

NCBI RefSeq record:
XM_006495989.3
NBCI Gene record:
Prkag3 (241113)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006495989.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000025123 CATCGGCACATTCCGAGATTT pLKO.1 1474 CDS 100% 13.200 18.480 N Prkag3 n/a
2 TRCN0000362322 ATCGGCACATTCCGAGATTTG pLKO_005 1475 CDS 100% 10.800 15.120 N Prkag3 n/a
3 TRCN0000362246 ACTCAAGTTCCTGCATATATT pLKO_005 1399 CDS 100% 15.000 12.000 N Prkag3 n/a
4 TRCN0000362321 AGATTGAAGAACATAAGATTG pLKO_005 1206 CDS 100% 10.800 7.560 N Prkag3 n/a
5 TRCN0000362323 AGTGCACCAGGACGATGAAAG pLKO_005 2069 3UTR 100% 10.800 7.560 N Prkag3 n/a
6 TRCN0000025119 GCTACTCAAGTTCCTGCATAT pLKO.1 1396 CDS 100% 10.800 7.560 N Prkag3 n/a
7 TRCN0000025122 GAGATGGACTTCTTAGAACAA pLKO.1 491 CDS 100% 4.950 3.465 N Prkag3 n/a
8 TRCN0000025121 CGTTGGAGATAAAGAAGGCTT pLKO.1 1044 CDS 100% 2.640 1.848 N Prkag3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006495989.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03391 pDONR223 100% 85.4% 84.6% None (many diffs) n/a
2 ccsbBroad304_03391 pLX_304 0% 85.4% 84.6% V5 (many diffs) n/a
3 TRCN0000480375 CCTACCATGGGAACCTAGAGTCGC pLX_317 26.9% 85.4% 84.6% V5 (many diffs) n/a
4 TRCN0000489566 GTAAAACGAGTCGCGCTGTTTTCA pLX_317 26.3% 85.4% 84.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_15077 pDONR223 99.7% 84.9% 35.3% None (many diffs) n/a
6 ccsbBroad304_15077 pLX_304 0% 84.9% 35.3% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000492015 CTTCTATTCAAAGCCTATCACCAC pLX_317 26.1% 84.9% 35.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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