Transcript: Human NM_017431.3

Homo sapiens protein kinase AMP-activated non-catalytic subunit gamma 3 (PRKAG3), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
PRKAG3 (53632)
Length:
2315
CDS:
34..1503

Additional Resources:

NCBI RefSeq record:
NM_017431.3
NBCI Gene record:
PRKAG3 (53632)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147132 TTAGGAGAGATGGAGACCAG pXPR_003 AGG 852 58% 8 1.1267 PRKAG3 PRKAG3 77296
2 BRDN0001146704 GCAGCTCAGAAAGAATCCGT pXPR_003 GGG 147 10% 3 0.4775 PRKAG3 PRKAG3 77294
3 BRDN0001145578 GGGTGGGCACTCCACCAACA pXPR_003 GGG 333 23% 4 0.3535 PRKAG3 PRKAG3 77295
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_017431.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006390 CTACCGCACTATCCAAGATTT pLKO.1 1065 CDS 100% 13.200 18.480 N PRKAG3 n/a
2 TRCN0000011014 CCAGATCTACATGCGCTTCAT pLKO.1 576 CDS 100% 4.950 6.930 N PRKAG3 n/a
3 TRCN0000006389 GCTAGTCATCTTCGACACCAT pLKO.1 639 CDS 100% 2.640 3.696 N PRKAG3 n/a
4 TRCN0000199400 CATCGGCACATTCCGAGACTT pLKO.1 1089 CDS 100% 4.950 3.465 N PRKAG3 n/a
5 TRCN0000195600 CCAAAGCCTTGAGATGGACAA pLKO.1 197 CDS 100% 4.050 2.835 N PRKAG3 n/a
6 TRCN0000196964 GAAGTGATCGACAGGATTGCT pLKO.1 1357 CDS 100% 3.000 2.100 N PRKAG3 n/a
7 TRCN0000197046 GCTGCTGAAATGCTGCATTTC pLKO.1 1968 3UTR 100% 1.080 0.756 N PRKAG3 n/a
8 TRCN0000006388 CCCTCCATTCTTGTCCAGAAA pLKO.1 2048 3UTR 100% 4.950 2.970 N PRKAG3 n/a
9 TRCN0000006391 CTCAGAAAGAATCCGTGGGAA pLKO.1 168 CDS 100% 2.640 1.584 N PRKAG3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017431.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03391 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03391 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480375 CCTACCATGGGAACCTAGAGTCGC pLX_317 26.9% 100% 100% V5 n/a
4 TRCN0000489566 GTAAAACGAGTCGCGCTGTTTTCA pLX_317 26.3% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_15077 pDONR223 99.7% 99.2% 48.6% None (many diffs) n/a
6 ccsbBroad304_15077 pLX_304 0% 99.2% 48.6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000492015 CTTCTATTCAAAGCCTATCACCAC pLX_317 26.1% 99.2% 48.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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