Construct: ORF ccsbBroadEn_15081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018912.1_s300c1, BRDN0000458208
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TRPM7 (54822)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54822 | TRPM7 | transient receptor potentia... | NM_001301212.2 | 96.6% | 12.1% | (many diffs) |
| 2 | human | 54822 | TRPM7 | transient receptor potentia... | NM_017672.6 | 96.6% | 12.1% | (many diffs) |
| 3 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022353.2 | 96.1% | 12% | (many diffs) |
| 4 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022352.1 | 96.1% | 12% | (many diffs) |
| 5 | human | 54822 | TRPM7 | transient receptor potentia... | XM_005254486.4 | 95.5% | 12% | (many diffs) |
| 6 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022351.1 | 95.1% | 11.9% | (many diffs) |
| 7 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022350.1 | 95.1% | 11.9% | (many diffs) |
| 8 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022354.1 | 92.5% | 8.9% | (many diffs) |
| 9 | human | 54822 | TRPM7 | transient receptor potentia... | XM_017022355.1 | 92.5% | 8.9% | (many diffs) |
| 10 | human | 54822 | TRPM7 | transient receptor potentia... | XR_001751327.1 | 81.4% | (many diffs) | |
| 11 | human | 54822 | TRPM7 | transient receptor potentia... | XR_002957654.1 | 76.7% | (many diffs) | |
| 12 | human | 54822 | TRPM7 | transient receptor potentia... | XR_001751325.1 | 75.6% | (many diffs) | |
| 13 | human | 54822 | TRPM7 | transient receptor potentia... | XR_001751328.2 | 75.3% | (many diffs) | |
| 14 | human | 54822 | TRPM7 | transient receptor potentia... | XR_001751326.2 | 75.3% | (many diffs) | |
| 15 | human | 54822 | TRPM7 | transient receptor potentia... | XR_002957653.1 | 61.8% | (many diffs) | |
| 16 | human | 54822 | TRPM7 | transient receptor potentia... | NR_149152.2 | 51.9% | (many diffs) | |
| 17 | human | 54822 | TRPM7 | transient receptor potentia... | NR_149153.2 | 51.2% | (many diffs) | |
| 18 | human | 54822 | TRPM7 | transient receptor potentia... | NR_149154.2 | 50.5% | (many diffs) | |
| 19 | mouse | 58800 | Trpm7 | transient receptor potentia... | NM_001164325.1 | 87.9% | 12% | (many diffs) |
| 20 | mouse | 58800 | Trpm7 | transient receptor potentia... | NM_021450.2 | 87.9% | 12% | (many diffs) |
| 21 | mouse | 58800 | Trpm7 | transient receptor potentia... | XM_017319154.1 | 73% | 14.4% | (many diffs) |
| 22 | mouse | 58800 | Trpm7 | transient receptor potentia... | XR_001783160.1 | 68.8% | (many diffs) | |
| 23 | mouse | 58800 | Trpm7 | transient receptor potentia... | XR_001783159.1 | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 846
- ORF length:
- 747
- Sequence:
-
1 CTATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GTCCCAGAAA TCCTGGATAG 121 AAAGCACTTT GACCAAGAGG GAATGTGTAT ATATTATACC AAGTTCCAAG GACCCTCACA 181 GATGCCTTCC AGGATGTCAA ATTTGTCAGC AACTCGTCAG GTGTTTTTGT GGTCGCTTGG 241 TCAAGCAACA TGCTTGTTTT ACTGCAAGTC TTGCCATGAA ATACTCAGAT GTGAAATTGG 301 GTGACCATTT TAATCAGGCA ATAGAAGAAT GGTCTGTGGA AAAGCATACA GAACAGAGCC 361 CAACGGATGC TTATGGAGTC ATAAATTTTC AAGGGGGTTC TCATTCCTAC AGAGCTAAGT 421 ATGTGAGGCT ATCATATGAC ACCAAACCTG AAGTCATTCT GCAACTTCTG CTTAAAGAAT 481 GGCAAATGGA GTTACCCAAA CTTGTTATCT CTGTACATGG GGGCATGCAG AAATTTGAGC 541 TTCACCCACG AATCAAGCAG TTGCTTGGAA AAGGTCTTAT TAAAGCTGCA GTTACAACTG 601 GAGCCTGGAT TTTAACTGGA GGAGTAAACA CAGGTGTGGC AAAACATGTT GGAGATGCCN 661 NNAAAGAACA TGCTTCNAGA TCATCTCGAA NNNTTTGCAC TATCGGAATA GCTCCATGGG 721 GAGTGATTGA AAACAGAAAT GATCTTGTTG GGAGAGATGT GGTTGCTCCT TATCAANCNC 781 TTATNNAACC CCCTGAGCAA ATTGAATGTT TTGAATNATC TGCATTCCCA TTTCATATTG 841 GTGGATGATG GCACTGTTGG AAAGTATGGG GCGGAAGTCA GACTGAGAAG AAGAACTTGA 901 AAAAACTTAT TAATCAGCAA AGAATTCATG CTAGGATTGG CCAGGGTGTC CCTGTGGTGG 961 CACTTATATT TGAGGGTGGG CCAAATGTTA TCCTCACAGT TCTTGAATAC CTTCAGGAAA 1021 GCCCCCCTGT TCCAGTAGTT GTGTGTGAAG GAACAGGCAG AGCTGCAGAT CTGCTAGCGT 1081 ATATTCATAA ACAAACAGAA GAAGGAGGGA ATCTTCCTGA TGCAGCAGAG CCCGATATTA 1141 TTTCCACTAT CAAAAAAACA TTTAACTTTG GCCAGAATGA AGCACTTCAT TTATTTCAAA 1201 CACTGATGGA GTGCATGAAA AGAAAGGAGC TTATCACTGT TTTCCATATT GGGTCAGATG 1261 AACATCAAGA TATAGATGTA GCAATACTTA CTGCACTGCT AAAAGATCTG AAGAGGAATG 1321 ATTATACGCC TGATAAAATT ATATTTCCTC AGGATGAGCC TTCAGATTTG AATCTTCAGC 1381 CTGGAAATTC CACCAAAGAA TCAGAATCAA CTAATTCTGT TCGTCTGATG TTATTGCCAA 1441 CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC 1501 AGGTCACTAT CAGTCAAAAT AAAATCATTA TTGCCATCCC AGCCTAAAAC tggaagaact 1561 ttacaacact aaacaaggtc caactaatcc aatgctgttt catcttgttc gagacgtcaa 1621 acagggaaat cttcctccag gatataagat cactctgatt gatataggac ttgttattga 1681 atatctcatg ggaggaacct acagatgcac ctatactagg aaacgttttc gattaatata 1741 taatagtctt ggtggaaata atcggaggtc tggccgaaat acctccagca gcactcctca 1801 gttgcgaaag agtcatgaat cttttggcaa tagggcagat aaaaaggaaa aaatgaggca 1861 taaccatttc attaagacag cacagcccta ccgaccaaag attgatacag ttatggaaga 1921 aggaaagaag aaaagaacca aagatgaaat tgtagacatt gatgatccag aaaccaagcg 1981 ctttccttat ccacttaatg aacttttaat ttgggcttgc cttatgaaga ggcaggtcat 2041 ggcccgtttt ttatggcaac atggtgaaga atcaatggct aaagcattag ttgcctgtaa 2101 gatctatcgt tcaatggcat atgaagcaaa gcagagtgac ctggtagatg atacttcaga 2161 agaactaaaa cagtattcca atgattttgg tcagttggcc gttgaattat tagaacagtc 2221 cttcagacaa gatgaaacca tggctatgaa attgctcact tatgaactga agaactggag 2281 taattcaacc tgccttaagt tagcagtttc ttcaagactt agaccttttg tagctcacac 2341 ctgtacacaa atgttgttat ctgatatgtg gatgggaagg ctgaatatga ggaaaaattc 2401 ctggtacaag gtcatactaa gcattttagt tccacctgcc atattgctgt tagagtataa 2461 aactaaggct gaaatgtccc atatcccaca atctcaagat gctcatcaga tgacaatgga 2521 tgacagcgaa aacaactttc agaacataac agaagagatc cccatggaag tgtttaaaga 2581 agtacggatt ttggatagta atgaaggaaa gaatgagatg gagatacaaa tgaaatcaaa 2641 aaagcttcca attacgcgaa agttttatgc cttttatcat gcaccaattg taaaattctg 2701 gtttaacacg ttggcatatt taggatttct gatgctttat acatttgtgg ttcttgtaca 2761 aatggaacag ttaccttcag ttcaagaatg gattgttatt gcttatattt ttacttatgc 2821 cattgagaaa gtccgtgaga tctttatgtc tgaagctggg aaagtaaacc agaagattaa 2881 agtatggttt agtgattact tcaacatcag tgatacaatt gccataattt ctttcttcat 2941 tggatttgga ctaagatttg gagcaaaatg gaactttgca aatgcatatg ataatcatgt 3001 ttttgtggct ggaagattaa tttactgtct taacataata ttttggtatg tgcgtttgct 3061 agattttcta gctgtaaatc aacaggcagg accttatgta atgatgattg gaaaaatggt 3121 ggccaatatg ttctacattg tagtgattat ggctcttgta ttacttagtt ttggtgttcc 3181 cagaaaggca atactttatc ctcatgaagc accatcttgg actcttgcta aagatatagt 3241 ttttcaccca tactggatga tttttggtga agtttatgca tacgaaattg atgtgtgtgc 3301 aaatgattct gttatccctc aaatctgtgg tcctgggacg tggttgactc catttcttca 3361 agcagtctac ctctttgtac agtatatcat tatggttaat cttcttattg catttttcaa 3421 caatgtgtat ttacaagtga aggcaatttc caatattgta tggaagtacc agcgttatca 3481 ttttattatg gcttatcatg agaaaccagt tctgcctcct ccacttatca ttcttagcca 3541 tatagtttct ctgttttgct gcatatgtaa gagaagaaag aaagataaga cttccgatgg 3601 accaaaactt ttcttaacag aagaagatca aaagaaactt catgattttg aagagcagtg 3661 tgttgaaatg tatttcaatg aaaaagatga caaatttcat tctgggagtg aagagagaat 3721 tcgtgtcact tttgaaagag tggaacagat gtgcattcag attaaagaag ttggagatcg 3781 tgtcaactac ataaaaagat cattacaatc attagattct caaattggcc atttgcaaga 3841 tctttcagcc ctgacggtag atacattaaa aacactcact gcccagaaag cgtcggaagc 3901 tagcaaagtt cataatgaaa tcacacgaga actgagcatt tccaaacact tggctcaaaa 3961 ccttattgat gatggtcctg taagaccttc tgtatggaaa aagcatggtg ttgtaaatac 4021 acttagctcc tctcttcctc aaggtgatct tgaaagtaat aatccttttc attgtaatat 4081 tttaatgaaa gatgacaaag atccccagtg taatatattt ggtcaagact tacctgcagt 4141 accccagaga aaagaattta attttccaga ggctggttcc tcttctggtg ccttattccc 4201 aagtgctgtt tcccctccag aactgcgaca gagactacat ggggtagaac tcttaaaaat 4261 atttaataaa aatcaaaaat taggcagttc atctactagc ataccacatc tgtcatcccc 4321 accaaccaaa ttttttgtta gtacaccatc tcagccaagt tgcaaaagcc acttggaaac 4381 tggaaccaaa gatcaagaaa ctgtttgctc taaagctaca gaaggagata atacagaatt 4441 tggagcattt gtaggacaca gagatagcat ggatttacag aggtttaaag aaacatcaaa 4501 caagataaaa atactatcca ataacaatac ttctgaaaac actttgaaac gagtgagttc 4561 tcttgctgga tttactgact gtcacagaac ttccattcct gttcattcaa aacaagaaaa 4621 aatcagtaga aggccatcta ccgaagacac tcatgaagta gattccaaag cagctttaat 4681 accggtttgg ttacaagata gaccatcaaa cagagaaatg ccatctgaag aaggaacatt 4741 aaatggtctc acttctccat ttaagccagc tatggataca aattactatt attcagctgt 4801 ggaaagaaat aacttgatga ggttatcaca gagcattcca tttacacctg tgcctccaag 4861 aggggagcct gtcacagtgt atcgtttgga agagagttca cccaacatac taaataacag 4921 catgtcttct tggtcacaac taggcctctg tgccaaaata gagtttttaa gcaaagagga 4981 gatgggagga ggtttacgaa gagctgtcaa agtacagtgt acctggtcag aacatgatat 5041 cctcaaatca gggcatcttt atattatcaa atcttttctt ccagaggtgg ttaatacatg 5101 gtcaagtatt tacaaagaag atacagttct gcatctctgt ctgagagaaa ttcaacaaca 5161 gagagcagca caaaagctta cgtttgcctt taatcaaatg aaacccaaat ccataccata 5221 ttctccaagg ttccttgaag ttttcctgct gtattgccat tcagcaggac agtggtttgc 5281 tgtggaagaa tgtatgactg gagaatttag aaaatacaac aataataatg gagatgagat 5341 tattccaact aatactctgg aagagatcat gctagccttt agccactgga cttacgaata 5401 tacaagaggg gagttactgg tacttgattt gcaaggtgtt ggtgaaaatt tgactgaccc 5461 atctgtgata aaagcagaag aaaagagatc ctgtgatatg gtttttggcc cagcaaatct 5521 aggagaagat gcaattaaaa acttcagagc aaaacatcac tgtaattctt gctgtagaaa 5581 gcttaaactt ccagatctga agaggaatga ttatacgcct gataaaatta tatttcctca 5641 ggatgagcct tcagatttga atcttcagcc tggaaattcc accaaagaat cagaatcaac 5701 taattctgtt cgtctgatgt tattgccaac tttcttgtac aaagttggca ttataagaaa 5761 gcattgctta tcaatttgtt gcaacgaac