Construct: ORF ccsbBroadEn_15094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018943.1_s300c1, BRDN0000458239
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4K2B (55300)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NM_018323.4 | 98.3% | 48.4% | (many diffs) |
| 2 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248174.2 | 97.5% | 48.9% | (many diffs) |
| 3 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248175.4 | 78.6% | 28.9% | (many diffs) |
| 4 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NR_144633.2 | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 837
- ORF length:
- 741
- Sequence:
-
1 GACGTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 61 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGA GGATCCCTCC GAGCCCGACC 121 GGTTGGCGTC CGCGGACGGC GGGAGCCCGG AGGAGGAGGA GGATGGGGAG CGGGAGCCGC 181 TGCTACCGCG GATCGCCTGG GCCCACCCGC GGAGAGGCGC CCCAGGCAGC GCCGTGAGGC 241 TGCTGGACGC TGCCGGGGAG GAGGGCGAGG CCGGCGACGA GGAGCTGCCC CTCCCGCCCG 301 GGGACGTGGG GGTCTCCCGG AGTTCGCCCG CCGAGCTGGA CCGGAGCCGC CCCGCGGTTT 361 CAGTAACTAT TGGTACTTCA GAGATGAATG CATTCTTGGA TGACCCAGAA TTTGCTGATA 421 TTATGCTGAG AGCAGAGCAA GCAATAGAAG TTGGAATTTT TCCAGAAAGA ATCTCTCAAG 481 GTTCAAGTGG AAGTTACTTT GTGAAGGATC CTAAGAGGAA AATTATTGGT GTGTTTAAAC 541 CCAAATCAGA AGAGCCTTAT GGTCAACTCA ATCCAAAATG GACCAAATAT GTCCATAAGG 601 TCTGCTGCCC TTGCTGCTTT GGCCGAGGCT GCCTGATTCC TAATCAGGGG TACCTTTCCG 661 AAGCGGGTGC CTATCTTGTG GACAACAAGC TTCATCTGAG CATTGTACCT AAAACAAAGG 721 TGGTTTGGCT TGTCAGTGAG ACATTTAACT ATAATGCGAT TGACCGTGCA AAATCAAGAG 781 GCAAAAAGTA TGCTTTAGAA AAAANNNNCN AAAGTTGGGT AGAAAGTTTC ATAGGATAGG 841 ACTCCTCTAA GTCTAAAATA TAGATGGTCC TTTCAGTTAT TTGTGAAGGT TACAAGGAGG 901 CTGAATATTG GCTTAGGAAA TTGAAGCTGA CCCTTTGCCT GAGAATATTA GAAAACAATT 961 TCAGTCACAA TTTGAAAGAT TAGTTATTTT GGATTACATC ATCAGAAATA CAGACAGGGG 1021 CAATGATAAT TGGTTAGTCA GATACGAAAA GCAGAAATGT GAAAAGGAAA TTGACCATAA 1081 GGAATCAAAA TGGATTGATG ATGAAGAATT CCTTATTAAA ATAGCTGCAA TTGATAATGG 1141 TCTAGCATTT CCTTTTAAAC ATCCTGATGA ATGGAGAGCA TATCCATTTC ACTGGGCTTG 1201 GCTTCCTCAA GCAAAAGTTC CCTTTTCTGA AGAAATAAGA AATTTGATTC TACCATATAT 1261 TTCTGACATG AACTTTGTGC AAGATTTATG TGAAGATCTC TATGAACTTT TTAAGACTGA 1321 CAAAGGATTT GACAAAGCCA CTTTTGAAAG TCAGATGTCT GTGATGAGGG GTCAGATCTT 1381 AAACCTTACT CAGGCATTGA GAGACGGGAA GAGTCCTTTC CAGCTAGTAC AGATACCTTG 1441 TGTGATTGTG GAACGCAGTC AAGGTGGAAG TCAGGGTCGG ATTGTCCACC TGAGCAATTC 1501 CTTTACCCAG ACTGTCAATT GCAGGAAGCC ATTTTTTTCC TCCTGGTTGC CAACTTTCTT 1561 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 1621 TATCAGTCAA AATAAAATCA TTATTGCCAT CCAGCGTGCT GAT