Construct: ORF ccsbBroadEn_15113

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011269.2_s300c1, BRDN0000458446
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPHK2 (56848)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56848 SPHK2 sphingosine kinase 2 NM_001204159.3 100% 100%
2 human 56848 SPHK2 sphingosine kinase 2 NM_020126.5 100% 100%
3 human 56848 SPHK2 sphingosine kinase 2 XM_011527133.1 100% 100%
4 human 56848 SPHK2 sphingosine kinase 2 NM_001204160.3 94.4% 94.4% 0_1ins108
5 human 56848 SPHK2 sphingosine kinase 2 XM_006723292.1 94.4% 94.4% 0_1ins108
6 human 56848 SPHK2 sphingosine kinase 2 XM_011527134.1 94.4% 94.4% 0_1ins108
7 human 56848 SPHK2 sphingosine kinase 2 NM_001204158.3 90.9% 90.9% 36_37ins177
8 human 56848 SPHK2 sphingosine kinase 2 XM_017027008.1 89.3% 89.8% (many diffs)
9 human 56848 SPHK2 sphingosine kinase 2 NM_001243876.1 68.5% 68.5% 0_1ins618
10 human 56848 SPHK2 sphingosine kinase 2 XM_017027010.2 54.3% 46.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2028
ORF length:
1962
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgaa tggacacctt gaagcagagg agcagcagga ccagaggcca gaccaggagc
121tgaccgggag ctggggccac gggcctagga gcaccctggt cagggctaag gccatggccc
181cgcccccacc gccactggct gccagcaccc cgctcctcca tggcgagttt ggctcctacc
241cagcccgagg cccacgcttt gccctcaccc ttacatcgca ggccctgcac atacagcggc
301tgcgccccaa acctgaagcc aggccccggg gtggcctggt cccgttggcc gaggtctcag
361gctgctgcac cctgcgaagc cgcagcccct cagactcagc ggcctacttc tgcatctaca
421cctaccctcg gggccggcgc ggggcccggc gcagagccac tcgcaccttc cgggcagatg
481gggccgccac ctacgaagag aaccgtgccg aggcccagcg ctgggccact gccctcacct
541gtctgctccg aggactgcca ctgcccgggg atggggagat cacccctgac ctgctacctc
601ggccgccccg gttgcttcta ttggtcaatc cctttggggg tcggggcctg gcctggcagt
661ggtgtaagaa ccacgtgctt cccatgatct ctgaagctgg gctgtccttc aacctcatcc
721agacagaacg acagaaccac gcccgggagc tggtccaggg gctgagcctg agtgagtggg
781atggcatcgt cacggtctcg ggagacgggc tgctccatga ggtgctgaac gggctcctag
841atcgccctga ctgggaggaa gctgtgaaga tgcctgtggg catcctcccc tgcggctcgg
901gcaacgcgct ggccggagca gtgaaccagc acgggggatt tgagccagcc ctgggcctcg
961acctgttgct caactgctca ctgttgctgt gccggggtgg tggccaccca ctggacctgc
1021tctccgtgac gctggcctcg ggctcccgct gtttctcctt cctgtctgtg gcctggggct
1081tcgtgtcaga tgtggatatc cagagcgagc gcttcagggc cttgggcagt gcccgcttca
1141cactgggcac ggtgctgggc ctcgccacac tgcacaccta ccgcggacgc ctctcctacc
1201tccccgccac tgtggaacct gcctcgccca cccctgccca tagcctgcct cgtgccaagt
1261cggagctgac cctaacccca gacccagccc cgcccatggc ccactcaccc ctgcatcgtt
1321ctgtgtctga cctgcctctt cccctgcccc agcctgccct ggcctctcct ggctcgccag
1381aacccctgcc catcctgtcc ctcaacggtg ggggcccaga gctggctggg gactggggtg
1441gggctgggga tgctccgctg tccccggacc cactgctgtc ttcacctcct ggctctccca
1501aggcagctct acactcaccc gtctccgaag gggcccccgt aattccccca tcctctgggc
1561tcccacttcc cacccctgat gcccgggtag gggcctccac ctgcggcccg cccgaccacc
1621tgctgcctcc gctgggcacc ccgctgcccc cagactgggt gacgctggag ggggactttg
1681tgctcatgtt ggccatctcg cccagccacc taggcgctga cctggtggca gctccgcatg
1741cgcgcttcga cgacggcctg gtgcacctgt gctgggtgcg tagcggcatc tcgcgggctg
1801cgctgctgcg ccttttcttg gccatggagc gtggtagcca cttcagcctg ggctgtccgc
1861agctgggcta cgccgcggcc cgtgccttcc gcctagagcc gctcacacca cgcggcgtgc
1921tcacagtgga cggggagcag gtggagtatg ggccgctaca ggcacagatg caccctggca
1981tcggtacact gctcactggg cctcctggct gcccggggcg ggagccctgc ccaactttct
2041tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

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