Transcript: Human NM_020126.5

Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
SPHK2 (56848)
Length:
2748
CDS:
152..2116

Additional Resources:

NCBI RefSeq record:
NM_020126.5
NBCI Gene record:
SPHK2 (56848)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146882 TCAGACACAGAACGATGCAG pXPR_003 GGG 1248 64% 7 0.573 SPHK2 SPHK2 77096
2 BRDN0001145867 AGGAGCCAAACTCGCCATGG pXPR_003 AGG 155 8% 3 0.3651 SPHK2 SPHK2 77094
3 BRDN0001148465 TTGACCAATAGAAGCAACCG pXPR_003 GGG 546 28% 4 0.3119 SPHK2 SPHK2 77093
4 BRDN0001146242 CTACGAAGAGAACCGTGCCG pXPR_003 AGG 442 22% 3 -0.2253 SPHK2 SPHK2 77095
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020126.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359275 CAGGATTGCGCTCGCTTTCAT pLKO_005 2235 3UTR 100% 5.625 7.875 N SPHK2 n/a
2 TRCN0000359274 GAGGGTAGTGCCTGATCAATG pLKO_005 2318 3UTR 100% 10.800 8.640 N SPHK2 n/a
3 TRCN0000036969 GCTTCGTGTCAGATGTGGATA pLKO.1 1164 CDS 100% 4.950 3.960 N SPHK2 n/a
4 TRCN0000359273 CTTCGTGTCAGATGTGGATAT pLKO_005 1165 CDS 100% 10.800 7.560 N SPHK2 n/a
5 TRCN0000359272 GGTTGCTTCTATTGGTCAATC pLKO_005 696 CDS 100% 10.800 7.560 N SPHK2 n/a
6 TRCN0000195359 CATCCAGACAGAACGACAGAA pLKO.1 802 CDS 100% 4.950 3.465 N SPHK2 n/a
7 TRCN0000036970 GTTGCTCAACTGCTCACTGTT pLKO.1 1051 CDS 100% 4.950 3.465 N SPHK2 n/a
8 TRCN0000036971 CTTCTATTGGTCAATCCCTTT pLKO.1 701 CDS 100% 4.050 2.835 N SPHK2 n/a
9 TRCN0000036973 CTACTTCTGCATCTACACCTA pLKO.1 490 CDS 100% 2.640 1.848 N SPHK2 n/a
10 TRCN0000036972 CTTTGTGCTCATGTTGGCCAT pLKO.1 1762 CDS 100% 2.160 1.512 N SPHK2 n/a
11 TRCN0000199038 CCAAGGCAGCTCTACACTCAC pLKO.1 1584 CDS 100% 1.350 0.945 N SPHK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020126.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15113 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15113 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468571 ACGACCGTCCCTTAAAGTGCGCCC pLX_317 16.5% 100% 100% V5 n/a
4 TRCN0000487788 GCGCACTGCGGATGATCGCCATGT pLX_317 11.5% 100% 100% V5 (not translated due to prior stop codon) n/a
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